Description
Proadrenomedullin (N-20) (PAMP-20) is an endogenous peptide with hypotensive activity.1 It consists of the 20 N-terminal amino acids of proadrenomedullin and is found primarily in human adrenal medulla and pheochromocytoma tissue, with small amounts present in the heart, lung, liver, spleen, pancreas, kidney, small intestine, and cerebral cortex.2 PAMP-20 is an agonist of the G protein-coupled orphan receptor MRGPRX2 that inhibits forskolin-induced cAMP accumulation in CHO cells expressing the human receptor with an EC50 value of 251 nM.3 It inhibits catecholamine secretion and sodium and calcium influx induced by the acetylcholine receptor (AChR) agonist carbachol (Item No. 14486) in bovine adrenal medullary cells in vitro (IC50s = 1.6, 2.5, and 1 μM, respectively).4 PAMP-20 induces a hypotensive response in anesthetized rats when administered at doses of 10, 20, and 50 nmol/kg by intravenous bolus injection.1 It also dose-dependently reduces systemic arterial and hindlimb perfusion pressure in anesthetized cats when injected intravenously or into the hindlimb perfusion circuit, respectively, indicating vasodilator activity.5WARNING This product is not for human or veterinary use.
References
[1] KAZUO KITAMURA . Identification and hypotensive activity of proadrenomedullin N-terminal 20 peptide (PAMP)[J]. FEBS Letters, 1994, 351 1: Pages 35-37. DOI: 10.1016/0014-5793(94)00810-8
[2] WASHIMINE H. Immunoreactive Proadrenomedullin N-Terminal 20 Peptide in Human Tissue, Plasma and Urine[J]. Biochemical and biophysical research communications, 1994, 202 2: Pages 1081-1087. DOI: 10.1006/bbrc.1994.2039
[3] MASAZUMI KAMOHARA . Identification of MrgX2 as a human G-protein-coupled receptor for proadrenomedullin N-terminal peptides[J]. Biochemical and biophysical research communications, 2005, 330 4: Pages 1146-1152. DOI: 10.1016/j.bbrc.2005.03.088
[4] FUMI KATOH. Proadrenomedullin N-Terminal 20 Peptide (PAMP), an Endogenous Anticholinergic Peptide: Its Exocytotic Secretion and Inhibition of Catecholamine Secretion in Adrenal Medulla[J]. Journal of Neurochemistry, 1995, 64 1: 459-461. DOI: 10.1046/j.1471-4159.1995.64010459.x
[5] H C CHAMPION. Proadrenomedullin NH2-terminal 20 peptide has direct vasodilator activity in the cat.[J]. American Journal of Physiology, 1997, 272 4 Pt 2: R1047-54. DOI: 10.1152/ajpregu.1997.272.4.r1047
