Subtilisin
- CAS No.
- 9014-01-1
- Chemical Name:
- Subtilisin
- Synonyms
- maxatase;EC 3.4.21.14;PROTEASE ALKALINE;EC 3.4.21.14;EC 3.4.21.14;EC 3.4.21.14;EC 3.4.21.14;EC 3.4.21.14;EC 3.4.21.14;protease from bacillus licheniformis
- CBNumber:
- CB1670037
- Molecular Formula:
- Unspecified
- Molecular Weight:
- 0
- MDL Number:
- MFCD00165548
- MOL File:
- Mol file
- MSDS File:
- SDS
| Density | 1.3 |
|---|---|
| vapor pressure | 0Pa at 25℃ |
| storage temp. | 2-8°C |
| form | powder |
| color | white |
| biological source | Bacillus sp. |
| Water Solubility | 100g/L at 25℃ |
| Specific Activity | 7-15units/mg solid |
| Stability | store cold |
| Major Application | food and beverages |
| Cosmetics Ingredients Functions |
SKIN CONDITIONING KERATOLYTIC |
| LogP | -3.1 at 25℃ |
| CAS DataBase Reference | 9014-01-1 |
| FDA UNII | 0ZP13EBC5Y |
| EPA Substance Registry System | Subtilisin (9014-01-1) |
| UNSPSC Code | 12352204 |
| NACRES | NA.84 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |     GHS05,GHS07,GHS08,GHS09 |
|---|---|
| Signal word | Danger |
| Hazard statements | H302-H315-H318-H334-H335-H410 |
| Precautionary statements | P261-P273-P280-P301+P312-P302+P352-P305+P351+P338 |
| target organs | Respiratory system |
| PPE | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn,N |
| Risk Statements | 37/38-41-42-36/37/38-50-22 |
| Safety Statements | 23-24-26-36/37/39-22-36/37-61-45-39 |
| RIDADR | 3082 |
| OEL | STEL: 0.00006 mg/m3 [60-minute] |
| WGK Germany | 2 |
| RTECS | UK9540000 |
| F | 3-10 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Acute Tox. 4 Oral Aquatic Acute 1 Aquatic Chronic 2 Eye Dam. 1 Resp. Sens. 1 Skin Irrit. 2 STOT SE 3 |
| Hazardous Substances Data | 9014-01-1(Hazardous Substances Data) |
| Toxicity | LD50 orl-rat: 3700 mg/kg FCTXAV 7,581,69 |
| REACH Registrations | Active |
Subtilisin price More Price(60)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | P3910 | Protease from Bacillus licheniformis lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A | 9014-01-1 | 500mg | $638 | 2026-04-30 | Buy |
| Sigma-Aldrich | P3910 | Protease from Bacillus licheniformis lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A | 9014-01-1 | 5x500mg | $2400 | 2026-04-30 | Buy |
| Sigma-Aldrich | P5985 | Protease from Bacillus sp. liquid, ≥16 U/g | 50 mL | $133 | 2025-07-31 | Buy | |
| Sigma-Aldrich | P5985 | Protease from Bacillus sp. liquid, ≥16 U/g | 250 mL | $258.3 | 2025-07-31 | Buy | |
| Sigma-Aldrich | P5860 | Protease from Bacillus sp. liquid, ≥8 U/g | 50 mL | $133 | 2025-07-31 | Buy |
Subtilisin Chemical Properties, Uses, Production
Defination
Proteases (EC 3.4.21.62) belong to the class of enzymes known as hydrolases, which catalyze hydrolysis of various bonds in presence of water. Proteases are also referred to as Peptidases or Proteinases. Proteases catalyze proteolysis of peptide bonds in polypeptides, proteins and selective hydrolysis of carboxylic esters and amino esters. There are different classes of Proteases, i.e. serine, threonine, cysteine, aspartate, glutamic acid and metallo – proteases.
Chemical Properties
These are proteolytic enzymes which take the form of light-colored, free-flowing powders. A protein containing numerous amino acids
Uses
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).
Uses
Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Uses
Protease from Bacillus sp. has been used to determine its proteolytic activity by spectrophotometric method.
Biosynthesis
Biosynthetically, subtilin is produced from a ribosomally derived pre-pro-peptide by a series of post-translational modifications. [1]
General Description
Proteolytic enzymes are known to possess catalytic, non-catalytic and ancillary domains. Proteases are broadly classified as endopeptidases and exopeptidases. Functionally they are divided as aspartic, glutamic, cysteine, threonine, serine and metalloproteases.
Biochem/physiol Actions
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typcially stabilized by Ca2+ and have high isoelectric points.
Safety Profile
Moderately toxic by ingestion. Aneye irritant. When heated to decomposition it emits toxicfumes of NOx.
Potential Exposure
These commercial proteolytic enzymes are used in laundry detergent formulations
Purification Methods
This alkaline protease is purified 211-fold by affinity chromatography using 4-(4-aminophenylazo)phenylarsonic acid complex to activated CH-Sepharose 4B. It is inhibited by 2-phenylethane boronic acid, PMSF, 3,4-dichloroisocoumarin, acetone and benzamide. [Chandraskaren & Dhar Anal Biochem 150 141 1985, Schomburg & Schomburg Springer Handbook of Enzymes 2nd Edn vol 7 p 286 2002.] Synexin (from bovine liver) M 47,000 Da. This Ca binding protein is purified by (NH4)2SO4 precipitation, then by a specific pH step elution from a chromatofocusing medium in the absence of ampholytes. The pI is 7.5. [Scott et al. Anal Biochem 149 163 1985.]
Incompatibilities
Incompatible with oxidizers (chlorates, nitrates, peroxides, permanganates, perchlorates, chlorine, bromine, fluorine, etc.); contact may cause fires or explosions. Keep away from alkaline materials, strong bases, strong acids, oxoacids, epoxides.
Toxics Screening Level
The initial threshold screening level (ITSL) for subtilisins is 0.0006 μg/m 3 (1-hour averaging time).
References
[1]Burrage, Sarah Anne. Biomimetic synthesis of subtilin. Diss. University of Southampton, 1998
Subtilisin Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai JONLN Reagent Co., Ltd. | 400-0066400 13621662912 | 422131432@qq.com | China | 9970 | 55 |
| Shanghai Yingxin laboratory equipment Co., Ltd | 021-021-59178156 15300768757 | 359382369@qq.com | China | 10667 | 58 |
| Amano Enzyme Manufacturing(China),Ltd. Shanghai Branch | 021-6249-0810 18931126866 | yuxian_guo@amano-enzyme.com.cn | China | 44 | 58 |
| Beijing OKA biological technology co., LTD | 010-62971590 18548936886 | 3462612863@qq.com | China | 10061 | 58 |
| Nanjing Dulai Biotechnology Co., Ltd. | 025-84699383-8003;025-846993838003-8003 18013301590 | njduly@126.com | China | 3293 | 55 |
| future industrial shanghai co., ltd | 400-0066400 13621662912 | sales@jonln.com | China | 1989 | 65 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Adamas Reagent, Ltd. | 400-6009262 15618770481 | yhx@titansci.com | China | 14098 | 59 |
| XiaoGan ShenYuan ChemPharm co,ltd | 15527621225; 15527621225 | 1791901229@qq.com | China | 6740 | 52 |
| Shandong Xiya Chemical Co., Ltd | 13355009207 13355009207 | 3007715519@qq.com | China | 18722 | 57 |
View Latest Price from Subtilisin manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-18 | Alkaline proteinase
9014-01-1
|
1g | 98.0% | 1kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-09-18 | EC 3.4.21.14
9014-01-1
|
0.99 | RongNa Biotechnology Co.,Ltd | ||||
 |
2025-06-27 | PROTEASE ALKALINE
9014-01-1
|
1KG | 99% | 500000kg | Hebei Chuanghai Biotechnology Co., Ltd |
-
- Alkaline proteinase
9014-01-1
- 98.0%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- EC 3.4.21.14
9014-01-1
- 0.99
- RongNa Biotechnology Co.,Ltd
-
- PROTEASE ALKALINE
9014-01-1
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
