Triptolide
- CAS No.
- 38748-32-2
- Chemical Name:
- Triptolide
- Synonyms
- (3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)-trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one;PG490;Triptolide 38748-32-2;Thunder God Vine Extract;8b,9,10-decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)-,(3bs,4as,5as,6r,6ar;triptolid;riptolide;TRIPTOLIDE;NSC 163062;TRIPTOLIDE95%
- CBNumber:
- CB2167404
- Molecular Formula:
- C20H24O6
- Molecular Weight:
- 360.4
- MDL Number:
- MFCD00210565
- MOL File:
- 38748-32-2.mol
- MSDS File:
- SDS
| Product description | Number | Pack Size | Price |
| Triptolide A novel diterpene triepoxide isolated from the Chinese herb | 645900 | 1mg | $153 |
| Triptolide phyproof? Reference Substance | PHL89811 | 10MG | $624 |
| Triptolide A novel diterpene triepoxide isolated from the Chinese herb | 645900 | 5mg | $576 |
| Triptolide >98.0%(HPLC) | T2899 | 10mg | $341 |
| Triptolide ≥98% | 11973 | 1mg | $32 |
| More product size | |||
| Melting point | 226-227°C |
|---|---|
| alpha | D25 -154° |
| Boiling point | 412.43°C (rough estimate) |
| Density | 1.1656 (rough estimate) |
| refractive index | 1.4450 (estimate) |
| storage temp. | 2-8°C |
| solubility | DMSO: soluble |
| form | solid |
| pka | 14.25±0.60(Predicted) |
| color | white to off-white |
| biological source | Tripterygium wilfordii |
| Merck | 14,9747 |
| Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 3 months. |
| InChIKey | DFBIRQPKNDILPW-CIVMWXNOSA-N |
| SMILES | O1[C@@]32[C@]5(O[C@H]5C[C@@H]6[C@@]3(CCC7=C6COC7=O)C)[C@@H]([C@]4(O[C@H]4[C@@H]21)C(C)C)O |
| CAS DataBase Reference | 38748-32-2(CAS DataBase Reference) |
| FDA UNII | 19ALD1S53J |
| EPA Substance Registry System | Trisoxireno[6,7:8a,9:4b,5]phenanthro[1,2-c]furan-1(3H)-one, 3b,4,4a,6,6a,7a,7b,8b,9,10-decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)-, (3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)- (38748-32-2) |
| UNSPSC Code | 41116107 |
| NACRES | NA.77 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS06,GHS08 |
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|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Danger | |||||||||
| Hazard statements | H300+H330-H361f | |||||||||
| Precautionary statements | P201-P202-P260-P264-P270-P304+P340+P310 | |||||||||
| Safety Statements | 22-24/25 | |||||||||
| RIDADR | 2811 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | YK7751000 | |||||||||
| HazardClass | 6.1 | |||||||||
| PackingGroup | I | |||||||||
| HS Code | 29321900 | |||||||||
| Storage Class | 6.1A - Combustible acute toxic Cat. 1 and 2 very toxic hazardous materials |
|||||||||
| Hazard Classifications | Acute Tox. 1 Inhalation Acute Tox. 1 Oral Repr. 2 |
|||||||||
| Toxicity | LD50 i.p. in mice: 1.93 mg/kg (Chen) | |||||||||
| NFPA 704 |
|
Triptolide price More Price(55)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 645900 | Triptolide A novel diterpene triepoxide isolated from the Chinese herb | 38748-32-2 | 1mg | $153 | 2025-07-31 | Buy |
| Sigma-Aldrich | PHL89811 | Triptolide phyproof? Reference Substance | 38748-32-2 | 10MG | $624 | 2025-07-31 | Buy |
| Sigma-Aldrich | 645900 | Triptolide A novel diterpene triepoxide isolated from the Chinese herb | 38748-32-2 | 5mg | $576 | 2025-07-31 | Buy |
| TCI Chemical | T2899 | Triptolide >98.0%(HPLC) | 38748-32-2 | 10mg | $341 | 2025-07-31 | Buy |
| Cayman Chemical | 11973 | Triptolide ≥98% | 38748-32-2 | 1mg | $32 | 2024-03-01 | Buy |
Triptolide Chemical Properties,Uses,Production
Tripterygium wilfordii
There are four species under the genus Tripterygium in the world, and all are native to China, with three species being medicine. Tripterygium wilfordii is a woody climber, 2-3 meters high. Branchlets are reddish and with brown angular, with oblong tuberculate projections and iron brown hairs. Flowering period is from May to June. Fruit ripening period is from August to September. It grows in shady and humid slopes, valleys, bushes besides streams and secondary miscellaneous forests, with the distribution in the south of the Yangtze River, Anhui, Zhejiang, Fujian, Taiwan, Jiangxi, Hunan, Guangdong and even the southwest provinces. It is harvested in summer or autumn. It is bitter and poisonous, and can be used as insecticide, anti-inflammatory and detoxification. This product is poisonous, thus be careful when orally taking in.
1.Treatment of rheumatoid arthritis: smash the roots and leaves of Tripterygium wilfordii and apply it externally. Remove it half an hour later, otherwise blister could occur. (Jiangxi, " Handbook of Herbs").
2. Treatment of skin itchiness: smash the leaves of Tripterygium wilfordii and apply it ( "Medicinal Flora in Hunan").
3. Treatment of snake strand sore: grind the flowers of Tripterygium wilfordii and the root of three-nerved spicebush, and apply it the affected area (“ Medicinal Flora in Hunan ").
Anti-inflammatory and immunosuppressive effects
Triptolide is an epoxy diterpene lactone compound that extracted from the roots, leaves, flowers and fruits of Tripterygium wilfordii (Celastraceae). Triptolide, with alkaloids like wilfordine, wilforine, wilforgine, Wilfortrine, wilfozine and Wilfornine, constitutes the main active ingredient of Tripterygium extraction. It is insoluble in water, soluble in methanol, dimethyl sulfoxide, ethanol, ethyl acetate, chloroform ect. It has anti-inflammatory and immunosuppressive effects: with the application of 10μg/kg sc Triptolide in continuous 10 days, the croton oil-induced ear edema in mice can be reduced. The in vitro tests with the application of 0.05~1μg/ml Triptolide in continuous 7 days showed this product has a stabilizing effect on sheep erythrocyte membrane; with the application of 75,150mg/kgsc Triptolide in continuous 7 days, the total complement levels of rat serum significantly increased, and the formation of hemolysin antibody in primary immune response was significantly inhibited, but no significant inhibition was found in the formation of hemolysin antibody in the second immune response; with the application of 150μg/kg ig Triptolide in continuous 7 days, a certain degree of inhibition in mouse peritoneal phagocytic function; with the application of 300μg/kg sc Triptolide in continuous 15 days, the croton oil-induced ear edema in mice was inhibited; with the application of 75,150μg/kgsc Triptolide in continuous 14 days, the carrageenan paw swelling in rat was inhibited; with the application of 0.22~4.44μg/ml Triptolide in Polygalatenuifolia extraction, a protective effect on RBCM of rats was showed; with the application of 0.25,1mg/kg ip Triptolide, antibody production and secretion in mice were inhibited (QHS method); with the application of 50,500ng/ml concentration, proliferative responses of T cell and B cell were significantly inhibited; with the application of 5,50,500ng/ml Triptolide in vitro and 0.5,0.75,1mg/kg ip drug in vivo, the production of mouse spleen cells IL-2 was significantly inhibited; with the application of 0.1~1μg/ml Triptolide in vitro, Con A-induced lymphocyte growth was inhibited; with the application of 0.5~10μg/L Triptolide the mixed lymphocyte culture (MLC) was inhibited: after 60Co irradiation, the mixed lymphocyte that induced with the concentration of 5,10μg/L Triptolide inhibited the second MLC, showing that triptolide can induce suppression T cells (Ts); with the application of 0.12~0.5mg/kg ip Triptolide, the DNFB-induced DTH in mice was significantly inhibited; with the application of 0.25,0.5mg/kg Triptolide, the secretion activity of mice spleen cells IL-2 was inhibited, and the increased DTH responses that induced by high-dose cyclophosphamide was also inhibited; with the application of 0.25mg/kg Triptolide, mouse thymus Th/Ts cell ratio reduced. Experimental results indicate that triptolide has immunosuppression, and the mechanism may be related to inhibition of Th cells and secretion activity of IL-2 and inducing Ts cells.
The above information is edited by the Chemicalbook of Cheng Jingmin.
Anti-cancer effects
In recent years, a large number of in vitro and in vivo studies demonstrated this product has good anti-tumor activity to a variety of cancers such as leukemia, breast cancer, pancreatic cancer and lung cancer. Recent studies have found that triptolide not only affects certain proteins and signaling pathways specifically, but also inhibits broad gene transcription, but the mechanism remains to be further clarified.
Yu Qiang group in Shanghai Institute of Materia Medica of Chinese Academy of Sciences studied in-depth the molecular mechanisms of Triptolide in suppressing broad gene transcription. The study found that Triptolide inhibited gene transcription by promoting the phosphorylation of the largest and most important functional subunit Rpb1 in RNA polymerase II, and subsequent Rpb1 ubiquitin degradation. PTEF-b, the upstream kinases of Rpb1, plays a positive role in the regulation of phosphorylation process of Rpb1 that induced by Triptolide. The study also found that Triptolide could induce DNA damage. These results indicate that triptolide suppresses broad gene transcription by causing DNA damage in cells and thus activating the P-TEFb, which can phosphorylate Rpb1 and cause Rpb1 degradation.
Using TLCS method to determine the content of triptolide
1. Chromatographic conditions: silica gel G-CMCNa plate; chloroform-ether (2: 1) as developing solvent; 2% 3,5-dinitro-benzoic acid ethanol solution and 2mol/L Sodium hydroxide ethanol solution. Mix them with the ratio 1: 3 before use as the chromogenic agent, spray the mixture and view the results under natural light.
2. The preparation of the reference solution: Weigh appropriate triptolide accurately as the reference substance, and add chloroform to make the reference solution with 1.528mg triptolide per ml.
3. Preparation of sample solution: Weigh appropriate dried herbs accurately, and make reflux extraction three times by adding ethanol, with 3 hours each time. Combine the extraction and recover ethanol to dryness, and then extract triptolide by dissolving in ethyl acetate until no triptolide presence in TLC, and recover ethyl acetate to get the extract. weigh accurately 0.4g extract powder, which has been dried at 90 ℃to constant weight and dissolve it in 5ml of dichloromethane, and mix with 8g neutral alumina, and evaporate the dichloromethane and transferre to a Soxhlet extractor, and reflux for 3h with petroleum ether (60~90 ℃), and discard the petroleum ether and remove the filter cartridge, shake off the solvent, and then reflux for 5h by placing it in an extractor with 70ml chloroform, recover chloroform to dryness and cool it, and dissolve it to precise 3ml chloroform as the sample solution.
4. Determination: precisely drawing 10μl sample solution and 2,7μl reference solution, and point them to the same piece of thin plate. According to the chromatographic conditions to spread, take out, dry and color. Cover it with the same size of glass plate and seal it for four cycles. 10min later, scan it under thin layer scanning. λS = 535nm, λR = 650nm; dual wavelength reflection zigzag scanning; slit: 1.25mm × 1.25mm; sensitivity × 5. Measure the absorption integral value of the sample and reference substance, and calculate the content by two external standard method.
5. Measurement results: Based on the above-mentioned conditions, the content of triptolide in Tripterygium root bark, root center and whole root were 0.001%, 0.0004%, 0.0018%.
Description
Triptolide (38748-32-2) possesses potent immunosuppressive and anti-inflammatory activity.? Irreversibly inhibits eukaryotic transcription via covalent binding to XPB, a subunit of the transcription factor TFIIH.1??Blocks transactivation of NFkB.2?Exhibits potent antiproliferative activity in 60 cancer cell lines (average IC50?= 12 nM) and synergizes with other anticancer agents.3?Inhibits the inflammatory response and remarkably decreases production of TNF-a, IL-1b and IL-6 in a rat model of rheumatoid arthritis.4
Chemical Properties
White Crystalline Solid
Uses
Labelled Triptolite (T815600), diterpenoid triepoxide with immunosuppressant and antitumor properties.
Uses
Triptolide is a diterpenoid triepoxide with immunosuppressant and antitumor properties.
Definition
ChEBI: Triptolide is an organic heteroheptacyclic compound, an epoxide, a gamma-lactam and a diterpenoid. It has a role as an antispermatogenic agent and a plant metabolite.
General Description
This substance is a primary reference substance with assigned absolute purity (considering chromatographic purity, water, residual solvents, inorganic impurities). The exact value can be found on the certificate. Produced by PhytoLab GmbH & Co. KG
Biological Activity
Inhibits cytokine-induced gene expression via inhibition of NF- κ B transcriptional activity. Induces apoptosis in tumor cells by blocking TNF- α mediated c-IAP2 and c-IAP1 (members of the inhibitor of apoptosis family) induction. Immunosuppressive with anti-inflammatory and antitumor activity.
Biochem/physiol Actions
Triptolide is a diterpene triepoxide, the principal active ingredient in extracts from the Chinese herb Tripterygium wilfordii hook (TwHF). It is a potent immunosuppressant and anti-inflammatory. Triptolide has been shown to inhibit the expression of IL-2 in activated T cells at the level of purine-box/nuclear factor and NF-κB mediated transcription activation. It synergizes with cyclosporin A in promoting graft survival in animal models and in suppression of graft versus host disease in allogeneic bone marrow transplants. In addition, it induces apoptosis in tumor cells and potentiates tumor necrosis factor (TNFα) induction of apoptosis in part through the suppression of c-IAP2 and c-IAP1 induction.
Synthesis
The herb of Lei Gongteng 300 kg (tested, its content of Lei Gongteng methylin is 0.0043%), crushed to 20-30 mesh, loaded into the Extraction tank, add 3 tons of ethyl acetate, then add 360g ascorbic acid, heating reflux extraction 3 times, each time 5 hours, extraction The liquid was filtered and concentrated under reduced pressure to obtain 8.3 kg of extract; the extract was dissolved with 360 liters of dichloromethane and beaten into the extraction tank, and another 17.28 was weighed. kg NaOH, prepared 720 liters of 0.6moL / LNaOH solution, in 2 times into the extraction tank, dichloromethane dissolved solution for _x000D _ Alkaline wash 2 times, concentrated dichloromethane phase to get 2 kg of alkaline wash after the dip; take 10 kg of 200 ~ 300 mesh Qingdao Ocean column chromatography positive phase Take 10 kg of 200-300 mesh Qingdao Ocean column chromatography positive phase silica gel, wet packing 35cm diameter column, after alkali washing extract on the sample, first eluted with dichloromethane, and after the color of the eluent becomes light, eluted with dichloromethane:methanol. Dichloromethane:methanol=50:1 mixture of solvents to elute 2 column volumes, and then eluted with methanol, concentrated methanol eluent to obtain methyl . The methanol eluent was concentrated to obtain 427g of methanol eluent; 20kg of 200-300 mesh Qingdao Ocean column chromatography normal phase silica gel was taken, and a 50cm diameter column was wet packed, and the methanol was used as the eluent. After the eluent was sampled, it was mixed with petroleum ether:ethyl acetate=10:1, 6:1, 4:1, 2:1, 1:1 (v/v) of in order of gradient elution. The solvent was subjected to a gradient elution of about 8 column volumes for each ratio, and the tretinoin-containing eluent was collected and concentrated to about 1/8 Volume after 4 ?? static precipitation crystal, obtained 13.8 g of crude tretinoin; the crude product with isopropyl ether as the crystallization solvent at room temperature for _x000D _ Recrystallization, crystallization is completed after filtration to obtain tretinoin pure 12.3g, HPLC detection of its purity of 99.4%.
storage
Store at -20°C
Background
Triptolide is an immunosuppressive and anti-inflammatory compound produced by the thunder god vine, Tripterygium wilfordii Hook F. Covalent binding of Triptolide to human XPB/ERCC3 inhibits its DNA-dependent ATPase activity and prevents nucleotide excision repair by the TFIIH complex. Triptolide has proven to be an effective synergistic therapy for inhibition of pancreatic cancer cell growth, with IC50 values of 0.01 µM and 0.02 µM in Capan-1 and Capan-2 adenocarcinoma cells, respectively. In addition to its anti-tumor activity, Triptolide has shown antiviral efficacy in HIV-1 and dengue virus via inhibition of Tat protein and COX-2, respectively.
References
[1] DR. QING-LI HE. Covalent Modification of a Cysteine Residue in the XPB Subunit of the General Transcription Factor TFIIH Through Single Epoxide Cleavage of the Transcription Inhibitor Triptolide†[J]. Angewandte Chemie International Edition, 2014, 54 6: 1859-1863. DOI:10.1002/anie.201408817
[2] K Y LEE. PG490 (triptolide) cooperates with tumor necrosis factor-alpha to induce apoptosis in tumor cells.[J]. The Journal of Biological Chemistry, 1999, 274 19: 13451-13455. DOI:10.1074/jbc.274.19.13451
[3] ZHIXIN QIAO. Synergistic antitumor activity of gemcitabine combined with triptolide in pancreatic cancer cells.[J]. Oncology Letters, 2016, 11 5: 3527-3533. DOI:10.3892/ol.2016.4379
[4] DANPING FAN. Triptolide Modulates TREM-1 Signal Pathway to Inhibit the Inflammatory Response in Rheumatoid Arthritis[J]. International Journal of Molecular Sciences, 2016, 88 1. DOI:10.3390/ijms17040498
Triptolide Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Chengdu GLP biotechnology Co Ltd | 028-87075086 13350802083 | scglp@glp-china.com | CHINA | 1824 | 58 |
| Chengdu Biopurify Phytochemicals Ltd. | +86-28-82633860; +8618080483897 | sales@biopurify.com | China | 3751 | 58 |
| Xi'an ZB Biotech Co.,Ltd | +8618591943808 | sales01@xazbbio.com | China | 815 | 58 |
| Hebei baicao biology science and technology co., ltd | +86-19131911055 +8617824879454 | zhang@hbbocao.com | China | 1029 | 58 |
| Shaanxi Haibo Biotechnology Co., Ltd | +undefined18602966907 | qinhe02@xaltbio.com | China | 997 | 58 |
| Anhui Ruihan Technology Co., Ltd | +8617756083858 | daisy@anhuiruihan.com | China | 973 | 58 |
| Wuhan JiyunZen Tech Co., Ltd. | +86-18062099985 | Amyjiyunzen@yeah.net | China | 672 | 58 |
| Capot Chemical Co.,Ltd. | +86-（0）57185586718; +8613336195806 | sales@capot.com | China | 29735 | 60 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21599 | 55 |
| Guangzhou PI PI BIOTECH INC | +86-86-020-81716320 +8613602409664 | Sales@pipitech.com | China | 973 | 55 |
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View Lastest Price from Triptolide manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-01-30 | Triptolide
38748-32-2
|
US $5.00-0.50 / KG | 1KG | 99% hplc | 500TONS | Wuhan JiyunZen Tech Co., Ltd. | |
 |
2025-04-04 | Triptolide
38748-32-2
|
US $0.00-0.00 / kg | 1kg | 98% | 1Ton | Henan Aochuang Chemical Co.,Ltd. | |
 |
2024-04-12 | Triptolide
38748-32-2
|
US $0.00 / kg | 1kg | 98% | 2000ton | Shaanxi Haibo Biotechnology Co., Ltd |
-
- Triptolide
38748-32-2
- US $5.00-0.50 / KG
- 99% hplc
- Wuhan JiyunZen Tech Co., Ltd.
-
- Triptolide
38748-32-2
- US $0.00-0.00 / kg
- 98%
- Henan Aochuang Chemical Co.,Ltd.
-
- Triptolide
38748-32-2
- US $0.00 / kg
- 98%
- Shaanxi Haibo Biotechnology Co., Ltd
