Diethyl pyrocarbonate
- CAS No.
- 1609-47-8
- Chemical Name:
- Diethyl pyrocarbonate
- Synonyms
- DEPC;DIETHYL DICARBONATE;Ethyl pyrocarbonate;DKD;Piref;Baycovin;DEP／DEPC;DEPC/DEP;ethylpyrocarbonate;Diethylpyrokarbonat
- CBNumber:
- CB2300384
- Molecular Formula:
- C6H10O5
Lewis structure
- Molecular Weight:
- 162.14
- MDL Number:
- MFCD00009106
- MOL File:
- 1609-47-8.mol
- MSDS File:
- SDS
| Product description | Number | Pack Size | Price |
| Diethyl dicarbonate for synthesis | 8.41751 | 5ml | $36.5 |
| Diethyl dicarbonate for synthesis | 8.41751 | 25mL | $134 |
| Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. | 298711 | 25g | $163 |
| Diethyl pyrocarbonate 97% | 159220 | 5g | $55.6 |
| Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. | 298711 | 100g | $562 |
| More product size | |||
| Melting point | 69℃ |
|---|---|
| Boiling point | 93-94 °C18 mm Hg(lit.) |
| Density | 1.12 g/mL at 20 °C |
| refractive index |
n |
| Flash point | 157 °F |
| storage temp. | 2-8°C |
| solubility | 95% ethanol: soluble4.5g/10 mL, clear, colorless |
| form | Liquid |
| color | APHA: ≤20 |
| biological source | synthetic (microbial fermentation/ cell culture) |
| Water Solubility | slow decomposition |
| Sensitive | Moisture Sensitive |
| Merck | 14,7998 |
| BRN | 637031 |
| Stability | Combustible. Unstable - readily decomposes. Incompatible with strong oxidizing agents, strong acids, strong reducing agents, strong bases, ammonia. |
| InChI | 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3 |
| InChIKey | FFYPMLJYZAEMQB-UHFFFAOYSA-N |
| SMILES | CCOC(=O)OC(=O)OCC |
| LogP | 1.101 (est) |
| CAS DataBase Reference | 1609-47-8(CAS DataBase Reference) |
| FDA 21 CFR | 189.140 |
| Substances Added to Food (formerly EAFUS) | DIETHYL PYROCARBONATE-- PROHIBITED |
| EWG's Food Scores | 1 |
| FDA UNII | LMR3LZG146 |
| NIST Chemistry Reference | Diethyl pyrocarbonate(1609-47-8) |
| EPA Substance Registry System | Dicarbonic acid, diethyl ester (1609-47-8) |
| UNSPSC Code | 12352202 |
| NACRES | NA.77 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H302 | |||||||||
| Precautionary statements | P264-P270-P301+P312-P501 | |||||||||
| Hazard Codes | Xn | |||||||||
| Risk Statements | 22-36/37/38-20/21/22 | |||||||||
| Safety Statements | 26-36-37/39 | |||||||||
| RIDADR | 2810 | |||||||||
| WGK Germany | 3 | |||||||||
| RTECS | LQ9350000 | |||||||||
| F | 4.4-9-21 | |||||||||
| TSCA | TSCA listed | |||||||||
| HazardClass | 6.1(b) | |||||||||
| PackingGroup | III | |||||||||
| HS Code | 29209085 | |||||||||
| Storage Class | 10 - Combustible liquids | |||||||||
| Hazard Classifications | Acute Tox. 4 Oral | |||||||||
| Toxicity | LD50 orally in Rabbit: 850 mg/kg | |||||||||
| Excepted Quantities | Non-Hazardous | |||||||||
| NFPA 704 |
|
Diethyl pyrocarbonate price More Price(47)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 8.41751 | Diethyl dicarbonate for synthesis | 1609-47-8 | 5ml | $36.5 | 2025-07-31 | Buy |
| Sigma-Aldrich | 8.41751 | Diethyl dicarbonate for synthesis | 1609-47-8 | 25mL | $134 | 2025-07-31 | Buy |
| Sigma-Aldrich | 298711 | Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. | 1609-47-8 | 25g | $163 | 2025-07-31 | Buy |
| Sigma-Aldrich | 159220 | Diethyl pyrocarbonate 97% | 1609-47-8 | 5g | $55.6 | 2025-07-31 | Buy |
| Sigma-Aldrich | 298711 | Diethyl Pyrocarbonate - CAS 1609-47-8 - Calbiochem Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca2+ permeability of SR vesicles. | 1609-47-8 | 100g | $562 | 2025-07-31 | Buy |
Diethyl pyrocarbonate Chemical Properties,Uses,Production
Chemical Properties
liquid
Uses
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.1
Uses
Gentle esterifying agent. Preservative for wines, soft drinks, fruit juices.
Uses
Diethyl pyrocarbonate may be used in the following studies:
- For the modification of histidyl residues in proteins.
- As a nuclease inhibitor, for the extraction of undegraded nucleic acids from etiolated and green plant tissues.
- For the modification of linear and supercoiled plasmid DNAs.
- As a chemical probe to investigate the secondary structure in negatively supercoiled DNA.
- For cabethoxylation of histidine residues of actin.
Definition
ChEBI: The diethyl ester of dicarbonic acid.
General Description
Diethyl pyrocarbonate (DEP) is a bactericidal agent and decomposes in water to CO2 and ethanol. DEP inhibits enzymes such as DNase and RNase without affecting nucleic acids. It is useful for the preparation of mRNA from tissues.
Biochem/physiol Actions
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
Safety Profile
Poison by ingestion, inhalation, and intraperitoneal routes. Concentrated DEPC is irritating to eyes, mucous membranes, and sh. When heated to decomposition it emits acrid smoke and fumes. See also ESTERS.
Purification Methods
Dissolve the ester in Et2O, wash it with dilute HCl, H2O, dry over Na2SO4, filter, evaporate and distil the residue first in vacuo then at atmospheric pressure. It is soluble in alcohols, esters, ketones and hydrocarbon solvents. A 50% w/w solution is usually prepared for general use. Treat with great CAUTION as DEP irritates the eyes, mucous membranes and skin. [Boehm & Mehta Chem Ber 71 1797 1938, Thoma & Rinke Justus Liebigs Ann Chem 624 30 1959, Beilstein 3 IV 18.]
Diethyl pyrocarbonate Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai Acmec Biochemical Technology Co., Ltd. | +86-18621343501; +undefined18621343501 | product@acmec-e.com | China | 33325 | 58 |
| Hebei Chuanghai Biotechnology Co., Ltd | +8615350571055 | Sibel@chuanghaibio.com | China | 8753 | 58 |
| Capot Chemical Co.,Ltd. | +86-（0）57185586718; +8613336195806 | sales@capot.com | China | 29735 | 60 |
| Shanghai Daken Advanced Materials Co.,Ltd | +86-2158073036 | info@dakenam.com | China | 13635 | 58 |
| Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 | info@tianfuchem.com | China | 21597 | 55 |
| ATK CHEMICAL COMPANY LIMITED | +undefined-21-51877795 | ivan@atkchemical.com | China | 33024 | 60 |
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29833 | 58 |
| Hubei xin bonus chemical co. LTD | 86-13657291602 | linda@hubeijusheng.com | CHINA | 22963 | 58 |
| Shandong chuangyingchemical Co., Ltd. | 18853181302 | sale@chuangyingchem.com | CHINA | 5906 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49977 | 58 |
Related articles
- Uses and Lewis Structure of Diethyl pyrocarbonate (DEPC)
- In molecular biology, Diethyl pyrocarbonate (DEPC) is mainly used as a strong, but not absolute, inhibitor of RNase activity.
- May 7，2025
View Lastest Price from Diethyl pyrocarbonate manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-01-30 | Diethyl pyrocarbonate
1609-47-8
|
US $1.00 / KG | 1KG | 99% | 10 mt | Hebei Chuanghai Biotechnology Co., Ltd | |
 |
2026-01-19 | DEPC
1609-47-8
|
US $0.00 / removed | 99.58% | 10g | TargetMol Chemicals Inc. | ||
 |
2023-07-10 | Diethyl pyrocarbonate
1609-47-8
|
US $306.00-204.00 / kg | 10kg | 99% | 100kg | Jiangsu Boquan Biotechnology Co., Ltd. |
-
- Diethyl pyrocarbonate
1609-47-8
- US $1.00 / KG
- 99%
- Hebei Chuanghai Biotechnology Co., Ltd
-
- DEPC
1609-47-8
- US $0.00 / removed
- 99.58%
- TargetMol Chemicals Inc.
-
- Diethyl pyrocarbonate
1609-47-8
- US $306.00-204.00 / kg
- 99%
- Jiangsu Boquan Biotechnology Co., Ltd.
