Sulfo-Cy5.5 amine

Description

Sulfo-Cy5.5 amine is a water-soluble dye that reacts with electrophilic substances and is very photostable. The addition of the amine group makes the compound reactive towards carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) etc.

Chemical Properties

Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 Lmol1cm1
Quantum yield (Φ): 0.21
Solubility: Easily soluble in organic solvents such as methanol and DMF, very soluble in water

Uses

Sulfo-Cyanine5.5 amine potassium is a water-soluble cyanine dye suitable for far-red/near-infrared applications such as in vivo imaging. The dye has four sulfonate groups, making it highly hydrophilic and water-soluble. Like other cyanines, sulfo-Cyanine5.5 has an excellent extinction coefficient, making it a bright fluorescent marker in the far-red region. This is an amine-containing fluorescent dye. The amine group is separated from the fluorophore by a relatively long linker that facilitates conjugation. Aliphatic primary amine groups can be coupled with various electrophiles (activated esters, epoxides, etc.) and can also be used for enzymatic transamination labeling.

References

[1] Khatri S, et al.Citrullinated Peptide Epitope Targets Therapeutic Nanoparticles to Human Neutrophils. Bioconjugate Chemistry, 2019, 30(10), 2584-2593.

Abs/Em Maxima

673/691 nm

Fluoresene quantum yield

0.21

Extinction Coefficient

211000

CF260

0.09

CF280

0.11

Raw materials

Preparation Products