Sulfo-Cyanine3.5 carboxylic acid

Description

sulfo-Cyanine3.5 is a cyanine fluorophore with fluorescence in the orange spectrum range. Its absorption and emission spectra are between those of Cyanine3 and Cyanine5.

This dye is a sulfonated form of Cyanine3.5 (contains four sulfo groups), so it has good solubility and can be used in reactions without adding organic solvents.

This reagent is a derivative with a free carboxyl group. Because of its inactivity in physiological conditions, sulfo-Cyanine3.5 carboxylic acid derivative can be used as a negative control in experiments with active dye derivatives. The carboxylic acid can be activated with carbodiimides (e. g. EDAC) and react with hydrazines, hydroxylamines, and amines.

Chemical Properties

Appearance: Dark purple solid
ex/em: 576/603 nm
Extinction coefficient (ε): 139000 Lmol1cm1
Quantum yield (Φ): 0.11

Abs/Em Maxima

576/603 nm

Fluoresene quantum yield

0.11

Extinction Coefficient

139000

CF260

0.16

CF280

0.17