JC-1

Description

JC-1 is a cationic carbocyanine dye that accumulates in the mitochondria of live cells in a potential-dependent manner.

The dye exists as a green-fluorescent monomer at depolarized membranes and low concentrations. At higher concentrations (aqueous solutions above 0.1 µM) and hyperpolarized membranes, the dye forms J-aggregates that exhibit an emission at the orange channel.

Healthy cells have high mitochondrial membrane potential, and the decrease of mitochondrial membrane potential is a marker of the early stage of apoptosis. All this allows the use of changes in the orange/green fluorescence ratio of JC-1 to determine healthy vs. depolarized mitochondria. The orange/green fluorescence ratio of JC-1 depends only on the mitochondrial membrane potential and not on other factors such as the size, shape, and density of mitochondria.

Uses

JC-1 is commonly used to determine mitochondrial membrane potential. It is a cationic carbocyanine dye that is membrane permeable and is often used for flow cytometry and fluorescent microscopy. This dye changes color as the membrane potential increases. At higher potentials, JC-1 forms aggregates, which changes the fluorescence emission color from green to red. Dyes and metabolites.

Definition

ChEBI: 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine iodide is a cyanine dye and an organic iodide salt. It has a role as a fluorochrome. It contains a 1,1',3,3'-tetraethyl-5,5',6,6'-tetrachloroimidacarbocyanine.

General Description

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ_m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨ_m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

Biochem/physiol Actions

Product does not compete with ATP.

storage

-20°C