Glucose-6-phosphate dehydrogenase
- CAS No.
- 9001-40-5
- Chemical Name:
- Glucose-6-phosphate dehydrogenase
- Synonyms
- G-6-PD;C:C&B31;G-6-P-DH;EC 1.1.1.49;EC: 1.1.1.49;IUB: 1.1.1.49;G-6-PDH TYPE V;ZWISCHENFERMENT;G-6-PDH TYPE IX;G-6-PDH TYPE XV
- CBNumber:
- CB3211458
- Molecular Formula:
- n.a.
- Molecular Weight:
- 0
- MDL Number:
- MFCD00081656
- MOL File:
- Mol file
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | ~120℃ |
|---|---|
| storage temp. | -20°C |
| form | suspension |
| color | white |
| biological source | yeast |
| Water Solubility | Soluble in water and dilute buffer. |
| Specific Activity | 550-1,100units/mg protein (biuret) |
| EPA Substance Registry System | Dehydrogenase, glucose 6-phosphate (9001-40-5) |
| UNSPSC Code | 12352204 |
| NACRES | NA.54 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261-P284-P304+P340+P312-P501 |
| PPE | Eyeshields, Gloves, type N95 (US) |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36-24/25 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 |
Glucose-6-phosphate dehydrogenase price More Price(70)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | G8529 | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret) | 9001-40-5 | 200units | $74.3 | 2026-04-30 | Buy |
| Sigma-Aldrich | G8529 | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret) | 9001-40-5 | 1000units | $192 | 2026-04-30 | Buy |
| Sigma-Aldrich | G8529 | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret) | 9001-40-5 | 2000units | $288 | 2026-04-30 | Buy |
| Sigma-Aldrich | G8529 | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, lyophilized powder, ≥550?units/mg protein (biuret) | 9001-40-5 | 10000units | $943 | 2026-04-30 | Buy |
| Sigma-Aldrich | G8404 | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550?units/mg protein (biuret) | 9001-40-5 | 200units | $76 | 2026-04-30 | Buy |
Glucose-6-phosphate dehydrogenase Chemical Properties,Uses,Production
Chemical Properties
White to yellow powder
Uses
Glucose-6-phosphate dehydrogenase, Leuconostoc mesenteroides is used to convert p-glucose-6-phosphate to p-glucono-d-lactone-6-phosphate in presence of NADP+ or NAD+. It is used as an intermediate, chemical and research purpose and also used fo analysis.
Uses
Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.
Uses
Glucose-6-phosphate dehydrogenase has been used in a study to investigate the effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells. Glucose-6-phosphate dehydrogenase has also been used in a study to investigate the incidence of apoptosis and transcript abundance in bovine follicular cells. Glucose-6-phosphate dehydrogenase is used to test ketose reductase activity in developing maize endosperm.
General Description
Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway. G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).
Biochem/physiol Actions
Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway<<<17>>>.
Purification Methods
The enzyme is useful for measuring pyridine nucleotides in enzyme recycling. The enzyme from Baker's yeast has been purified by (NH4)2SO4 fractionation, Me2CO precipitation, a second (NH4)2SO4 fractionation, concentration by DEAE-SF chromatography, a third (NH4)2SO4 fractionation and recrystallisation. Crystallisation is induced by addition of its coenzyme NADP, which in its presence causes rapid separation of crystals at (NH4)2SO4 concentration much below that required to precipitate the amorphous enzyme. To recrystallise, the crystals are dissolved in 0.01M NADP (pH 7.3) with (NH4)2SO4 at 0.55 saturation, and the crystals appear within 10 to 60minutes. After standing for 2-3days (at 4o) the (NH4)2SO4 is increased to 0.60 of saturation, and more than 80% of the activity in the original crystals is recovered in the fresh crystals. [Noltmann et al. J Biol Chem 236 1255 1961]. Large amounts can be obtained from rat livers. The livers are extracted with 0.025M phosphate buffer (pH 7.5) and precipitated with 3M (NH4)2SO4 (70% of activity). The precipitate is dissolved in 3volumes of 0.025M phosphate (pH 7.5), dialysed against this buffer + 0.2mM EDTA at 4o for 5hours, then diluted to 1% protein and the nucleic acids are precipitated by addition of 0.4volumes of 1% protamine sulfate. (NH4)2SO4 is added to a concentration of 2M (pH adjusted to 7.0 with NH3), the precipitate is discarded and the supernatant is adjusted to 2.8M (NH4)2SO4, dialysed, and the protein is adjusted to 1% and treated with Ca3(PO4)2 gel. The gel is added in three steps (1.5mL of 0.4% gel/mL per step), and the gel is removed by centrifugation after each addition. The third gel adsorbed 50% of the activity. The gel is eluted with 0.2M phosphate buffer (pH 7.4, 40mL/g of gel; 60% recovery). The extract is precipitated in 3volumes of (NH4)2SO4 (adjusted to 4M) to give enzyme with an activity of 30Wmoles/mg of protein per hour. [Lowry et al. J Biol Chem 236 2746 1961.] The Km values for the yeast enzyme are 20WM for G-6P and 2WM for NADP (Tris pH 8.0, 10-2 M MgCl2, 38o) [Noltmann & Kuby The Enzymes VII 223 1963].
Glucose-6-phosphate dehydrogenase Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| ShangHai YuanYe Biotechnology Co., Ltd. | 15026964105 | shyysw007@163.com | China | 4998 | 60 |
| Shanghai Baoman Biotechnology Co., Ltd. | 021-62130998 | baomanbio@163.com | China | 199 | 55 |
| Shanghai Guchen Biotechnology Co., LTD | 021-34675735 19147740836 | 1986399151@qq.com | China | 9839 | 58 |
| Shanghai Biological Technology Development Co., Ltd. | 021-69955236-807 13918189704 | chinaruji@chinaruji.com | China | 5093 | 55 |
| Hubei wei shi reagent group ltd., company | 17771898297 13125137661 | 2853877621@qq.com | China | 7993 | 58 |
| Nanjing Dulai Biotechnology Co., Ltd. | 025-84699383-8003;025-846993838003-8003 18013301590 | njduly@126.com | China | 3293 | 55 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 18930552037 | 3bsc@sina.com | China | 15813 | 69 |
| Adamas Reagent, Ltd. | 400-6009262 15618770481 | yhx@titansci.com | China | 14098 | 59 |
| Shandong Xiya Chemical Co., Ltd | 13355009207 13355009207 | 3007715519@qq.com | China | 18722 | 57 |
View Lastest Price from Glucose-6-phosphate dehydrogenase manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2019-07-12 | GLUCOSE-6-PHOSPHATE DEHYDROGENASE
9001-40-5
|
$1.00 | 1KG | 99% | 25kg | Career Henan Chemical Co |
-
- GLUCOSE-6-PHOSPHATE DEHYDROGENASE
9001-40-5
- $1.00
- 99%
- Career Henan Chemical Co
