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Dihydrorhodamine 123

Dihydrorhodamine 123 structure

CAS No.: 109244-58-8

Chemical Name:: Dihydrorhodamine 123

Synonyms: DHR123;DHR;Dihydrorhodamine;D 632;D 23806;CAS:109244-58-8;Dihydrohodamine 123;Dihydrorhodamine 123;Dihydrorhodamine123(DHR123);Dihydrorhodamine 123, 10 mM in DMSO

CBNumber:: CB3320004

Molecular Formula:: C21H18N2O3

Molecular Weight:: 346.38

MDL Number:: MFCD04221428

MOL File:: 109244-58-8.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-09-12 18:51:59

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Dihydrorhodamine 123 Properties

Melting point	163–165℃
Boiling point	526.9±50.0 °C(Predicted)
Density	1.313±0.06 g/cm3(Predicted)
storage temp.	-20°C
solubility	DMSO: >5mg/mL
form	powder
pka	4.70 ± 0.40, most basic, temperature: 25 °C
color	Light red or pinkish-white
λmax	289 nm
BRN	5989965
Biological Applications	Generating,detecting & measuring reactive oxygen species; generating,detecting & measuring reactive nitrogen species; detecting phosphates,peroxynitrile; measuring respiratory burst; chronic granulomatous disease DHR assay; screening
Major Application	diagnostic assay manufacturing hematology histology
InChI	InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
InChIKey	FNEZBBILNYNQGC-UHFFFAOYSA-N
SMILES	C(OC)(=O)C1=CC=CC=C1C1C2=C(C=C(N)C=C2)OC2=C1C=CC(N)=C2
UNSPSC Code	12352116
NACRES	NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)	GHS07
Signal word	Warning
Hazard statements	H319-H302-H315-H335
Precautionary statements	P264-P280-P302+P352-P321-P332+P313-P362-P264-P280-P305+P351+P338-P337+P313P-P264-P270-P301+P312-P330-P501
PPE	Eyeshields, Gloves, type N95 (US)
Safety Statements	22-24/25
WGK Germany	3
F	10-23
Storage Class	11 - Combustible Solids

Dihydrorhodamine 123 price More Price(25)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	D1054	Dihydrorhodamine 123 ≥95%	109244-58-8	2mg	$142	2026-04-30	Buy
Sigma-Aldrich	D1054	Dihydrorhodamine 123 ≥95%	109244-58-8	10mg	$448	2026-04-30	Buy
Cayman Chemical	85100	Dihydrorhodamine 123 ≥98%	109244-58-8	1mg	$41	2026-04-30	Buy
Cayman Chemical	85100	Dihydrorhodamine 123 ≥98%	109244-58-8	5mg	$108	2026-04-30	Buy
Cayman Chemical	85100	Dihydrorhodamine 123 ≥98%	109244-58-8	10mg	$196	2026-04-30	Buy

Product number	Packaging	Price	Buy
D1054	2mg	$142	Buy
D1054	10mg	$448	Buy
85100	1mg	$41	Buy
85100	5mg	$108	Buy
85100	10mg	$196	Buy

Dihydrorhodamine 123 Chemical Properties, Uses, Production

Description

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M⁻¹cm⁻¹). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.

Uses

Dihydrorhodamine 123 is the uncharged and nonfluorescent reduction product of the mitochondrion-selective dye rhodamine 123 used for investigation of reacitve oxygen intermediates.

Uses

Dihydrorhodamine 123 (DHR) has been used for the detection of free intracellular radicals or ROS (reactive oxygen species) in head kidney-derived macrophages. It has also been used in DHR assay in red blood cells and in oxidative burst assay in neutrophils.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. Dihydrorhodamine is not fluorescent unless it is converted to rhodamine 123.

Biochem/physiol Actions

Cell permeable: yes

References

[1] CROW J P. Dichlorodihydrofluorescein and Dihydrorhodamine 123 Are Sensitive Indicators of Peroxynitritein Vitro:Implications for Intracellular Measurement of Reactive Nitrogen and Oxygen Species[J]. Nitric oxide: biology and chemistry, 1997, 1 2: Pages 145-157. DOI: 10.1006/niox.1996.0113
[2] K BRIVIBA. Attenuation of oxidation and nitration reactions of peroxynitrite by selenomethionine, selenocystine and ebselen.[J]. Biochemical Journal, 1996, 319 ( Pt 1): 13-15. DOI: 10.1042/bj3190013
[3] H SIES. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase.[J]. The Journal of Biological Chemistry, 1997, 272 44: 27812-27817. DOI: 10.1074/jbc.272.44.27812
[4] OSAMU HANDA Gediminas C Jancy Stephen. Role of endothelial nitric oxide synthase-derived nitric oxide in activation and dysfunction of cerebrovascular endothelial cells during early onsets of sepsis.[J]. American journal of physiology. Heart and circulatory physiology, 2008, 295 4: H1712-9. DOI: 10.1152/ajpheart.00476.2008
[5] PAIGE LACY. Divergence of mechanisms regulating respiratory burst in blood and sputum eosinophils and neutrophils from atopic subjects.[J]. Journal of immunology, 2003, 170 5: 2670-2679. DOI: 10.4049/jimmunol.170.5.2670
[6] JIANRONG LI. Peroxynitrite generated by inducible nitric oxide synthase and NADPH oxidase mediates microglial toxicity to oligodendrocytes.[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102 28: 9936-9941. DOI: 10.1073/pnas.0502552102

Dihydrorhodamine 123 Preparation Products And Raw materials

Raw materials

Preparation Products

Dihydrorhodamine 123 Suppliers

Global Suppliers ( 166)

Supplier	Tel	Email	Country	ProdList	Advantage
Nantong Hi-Future Biotechnology Co., Ltd	+86-0513; 18051384581	sales@chemhifuture.com	China	3075	58
J & K SCIENTIFIC LTD.	18210857532 18210857532	jkinfo@jkchemical.com	China	96815	76
Beijing Ouhe Technology Co., Ltd	13552068683 13522913783	2355560935@qq.com	China	11777	60
Syntechem Co.,Ltd		info@syntechem.com	China	12973	57
Dalian Meilun Biotech Co., Ltd.	0411-62910999 13889544652	sales@meilune.com	China	4765	58
Shanghai Aladdin Bio-Chem Technology Co.,LTD	400-6206333 13167063860	anhua.mao@aladdin-e.com	China	29985	65
TargetMol Chemicals Inc.	021-021-33632979 15002134094	marketing@targetmol.com	China	7993	58
Quality Control Solutions Ltd.	0755-66853366 13670046396	orders@qcsrm.com	China	18188	56
Shanghai CanSpecsci Instrument Co., Ltd.	400-6087598 15021221957	order@canspecsci.com	China	2001	56
Shanghai Macklin Biochemical Co.,Ltd.	15221275939 15221275939	shenlinxing@macklin.cn	China	15775	55

Supplier	Advantage
Nantong Hi-Future Biotechnology Co., Ltd	58
J & K SCIENTIFIC LTD.	76
Beijing Ouhe Technology Co., Ltd	60
Syntechem Co.,Ltd	57
Dalian Meilun Biotech Co., Ltd.	58
Shanghai Aladdin Bio-Chem Technology Co.,LTD	65
TargetMol Chemicals Inc.	58
Quality Control Solutions Ltd.	56
Shanghai CanSpecsci Instrument Co., Ltd.	56
Shanghai Macklin Biochemical Co.,Ltd.	55

View Latest Price from Dihydrorhodamine 123 manufacturers

Image	Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
	2026-09-10	Dihydrorhodamine 123 109244-58-8	$33.00-112.00		99.96%	10g	TargetMol Chemicals Inc.

Dihydrorhodamine 123
109244-58-8
$33.00-112.00
99.96%
TargetMol Chemicals Inc.

Dihydrorhodamine 123 Spectrum

DIHYDRORHODAMINE 123(109244-58-8)¹HNMR

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2-(3,6-DIAMINO-9H-XANTHEN-9-YL)-BENZOIC ACID, METHYL ESTER Dihydrohodamine 123 DIHYDRORHODAMINE 123, FOR FLUOR-ESCENCE Dihydrorhodamine123(DHR123) Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate D 23806 D 632 Dihydrorhodamine 123 *CAS 109244-58-8* Dihydrorhodamine 123 - CAS 109244-58-8 - Calbiochem Dihydrorhodamine 123, Fluorescent probe for ROS Dihydrorhodamine 123, 10 mM in DMSO CAS:109244-58-8 DHR123 DHR Dihydrorhodamine Dihydrorhodamine 123 109244-58-8 BioChemical Biochemicals and Reagents Fluorescent Enzyme Substrates Fluorescent Probes, Labels, Particles and Stains Alphabetical Xanthene AlphabeticalStains and Dyes DFluorescent Probes, Labels, Particles and Stains Fluorescent Enzyme Substrates Fluorescent Enzyme Substrates for Flow Cytometry Fluorescent Probes, Labels, Particles and Stains Stains&Dyes, A to