LUCIGENIN | 2315-97-1

LUCIGENIN Properties

Melting point	250°C
storage temp.	room temp
solubility	acetic acid: soluble10mg/mL
form	powder
color	Yellow powder with orange to brown cast
λmax	455nm
BRN	3901768
Biological Applications	Chloride indicator; diagnosis of hemostatic disorders; detecting bacteria,nucleicacids,proteins,pathogens; identifying respiratory infections; generating and detecting reactive oxygen species; chemiluminescent indicator
Major Application	diagnostic assay manufacturing hematology histology
InChI	InChI=1S/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28;22-1(3)4/h3-18H,1-2H3;;/q+2;2-1
InChIKey	KNJDBYZZKAZQNG-UHFFFAOYSA-N
SMILES	C1(=C2C=CC=CC2=[N+](C)C2=CC=CC=C12)C1=C2C=CC=CC2=[N+](C)C2=CC=CC=C12.[N+]([O-])([O-])=O.[N+]([O-])([O-])=O
UNSPSC Code	12171500
NACRES	NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS03,GHS07

Signal word

Warning

Hazard statements

H272-H315-H319

Precautionary statements

P210-P220-P221-P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P501

PPE

Eyeshields, Gloves, type N95 (US)

Safety Statements

24/25

RIDADR

1479

WGK Germany

3

HazardClass

5.1

PackingGroup

III

HS Code

29339900

Storage Class

11 - Combustible Solids

NFPA 704

	0
2		0

LUCIGENIN price More Price(40)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	M8010	N,N′-Dimethyl-9,9′-biacridinium dinitrate used as chemiluminescent reagent	2315-97-1	1g	$435	2026-04-30	Buy
Sigma-Aldrich	M8010	N,N′-Dimethyl-9,9′-biacridinium dinitrate used as chemiluminescent reagent	2315-97-1	5g	$1410	2026-04-30	Buy
TCI Chemical	B1203	10,10'-Dimethyl-9,9'-biacridinium Dinitrate [for Chemiluminescence Research] >97.0%(HPLC)	2315-97-1	1g	$137	2026-04-30	Buy
TCI Chemical	B1203	10,10'-Dimethyl-9,9'-biacridinium Dinitrate [for Chemiluminescence Research] >97.0%(HPLC)	2315-97-1	5g	$412	2026-04-30	Buy
Cayman Chemical	14872	Lucigenin ≥95%	2315-97-1	50mg	$36	2026-04-30	Buy

Product number	Packaging	Price	Buy
M8010	1g	$435	Buy
M8010	5g	$1410	Buy
B1203	1g	$137	Buy
B1203	5g	$412	Buy
14872	50mg	$36	Buy

LUCIGENIN Chemical Properties,Uses,Production

Description

Lucigenin is a chemiluminescent probe used to detect superoxide production and the presence of chloride. It can be used to detect superoxide production by enzymatic and cellular sources. It is a sensitive method that has been applied to the monitoring of superoxide production from xanthine oxidase, microsomal NADPH cytochrome reductase, and NADPH oxidases of phagocytes, endothelial cells, fibroblasts, and smooth muscle cells of blood vessel walls. However, it produces similar chemiluminescence signals in isolated aortic and cardiac tissues from wild-type and Nox1-Nox2-Nox4 triple knockout mice, suggesting that it is not selective for NADPH-based ROS production. It also reacts with hydrogen peroxide without generating free radical intermediates and has been used to detect lipid hydroperoxide in oils. Lucigenin is also used as a fluorescent chloride-sensitive indicator, with its fluorescence being quenched by chloride (ex/em = 455/505 nm, respectively). Lucigenin fluorescence is insensitive to phosphate, sulfate, and nitrate.

Chemical Properties

Dark yellow powder

Uses

Lucigenine, is used as chemiluminescence. It exhibits a bluish-green fluorescence. Dyes and metabolites.

Uses

Chemiluminescent probe for the detection of peroxides in biological systems.

Mechanism of action

In the case of lucigenin it is first reduced to a cation radical. Then it reacts in its univalently reduced form with ROS to produce dioxetane, which breaks down to generate photons. These probes cannot measure the individual free radicals but measure global ROS levels in the sample. Luminescence signals are expressed in relative light units (RLU), a direct measure of free radicals produced.

References

[1] Y LI. Validation of lucigenin (bis-N-methylacridinium) as a chemilumigenic probe for detecting superoxide anion radical production by enzymatic and cellular systems.[J]. The Journal of Biological Chemistry, 1998, 273 4: 2015-2023. DOI: 10.1074/jbc.273.4.2015
[2] BIWERSI J. Verkman A S Tulk B. Long-Wavelength Chloride-Sensitive Fluorescent Indicators[J]. Analytical biochemistry, 1994, 219 1: Pages 139-143. DOI: 10.1006/abio.1994.1242
[3] YU. A. VLADIMIROV E. V P. Free radicals and cell chemiluminescence[J]. Biochemistry (Moscow), 2010, 74 13: 1545-1566. DOI: 10.1134/s0006297909130082
[4] MIKHAIL P. SKATCHKOV . Validation of Lucigenin as a Chemiluminescent Probe to Monitor Vascular Superoxide as Well as Basal Vascular Nitric Oxide Production[J]. Biochemical and biophysical research communications, 1999, 254 2: Pages 319-324. DOI: 10.1006/bbrc.1998.9942
[5] HUA CAI. Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: comparison of sensitivity and specificity.[J]. Methods in molecular medicine, 2007, 139: 293-311. DOI: 10.1007/978-1-59745-571-8_20
[6] FLáVIA REZENDE. Unchanged NADPH Oxidase Activity in Nox1-Nox2-Nox4 Triple Knockout Mice: What Do NADPH-Stimulated Chemiluminescence Assays Really Detect[J]. Antioxidants & redox signaling, 2016, 24 7: 392-399. DOI: 10.1089/ars.2015.6314
[7] JOHN P. BUNTING David A G. Development of a flow injection chemiluminescent assay for the quantification of lipid hydroperoxides[J]. Journal of the American Oil Chemists Society, 2003, 80 10: 951-955. DOI: 10.1007/s11746-003-0802-1
[8] FERDANI R, LI R, PAJEWSKI R, et al. Transport of chloride and carboxyfluorescein through phospholipid vesicle membranes by heptapeptide amphiphiles[J]. Organic & Biomolecular Chemistry, 2007, 15: 2423-2432. DOI: 10.1039/b705544g
[9] RUEDAS-RAMA M J, ORTE A, HALL E A H, et al. A chloride ion nanosensor for time-resolved fluorimetry and fluorescence lifetime imaging[J]. Analyst, 2012, 6: 1500-1508. DOI: 10.1039/c2an15851e

LUCIGENIN Preparation Products And Raw materials

Raw materials

Preparation Products

LUCIGENIN Suppliers

Global( 189)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Henan Lavoisier Chemical Products Co. Ltd	0371-63682289 18625781376	sales@lavoisier.cn	China	4999	58
J & K SCIENTIFIC LTD.	18210857532 18210857532	jkinfo@jkchemical.com	China	96815	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	4006356688 18621169109	market03@meryer.com	China	40202	62
3B Pharmachem (Wuhan) International Co.,Ltd.	18930552037	3bsc@sina.com	China	15813	69
future industrial shanghai co., ltd	400-0066400 13621662912	sales@jonln.com	China	1989	65
TCI (Shanghai) Development Co., Ltd.	021-67121386	Sales-CN@TCIchemicals.com	China	24512	81
Energy Chemical	400-0056266	sales8178@energy-chemical.com	China	44850	61
Sichuan Kulinan Technology Co., Ltd	400-1166-196 18981987031	cdhxsj@163.com	China	11674	57
Tianjin heowns Biochemical Technology Co., Ltd.	400 638 7771	sales@heowns.com	China	14412	57
Dalian Meilun Biotech Co., Ltd.	0411-62910999 13889544652	sales@meilune.com	China	4765	58

Supplier	Advantage
Henan Lavoisier Chemical Products Co. Ltd	58
J & K SCIENTIFIC LTD.	76
Meryer (Shanghai) Chemical Technology Co., Ltd.	62
3B Pharmachem (Wuhan) International Co.,Ltd.	69
future industrial shanghai co., ltd	65
TCI (Shanghai) Development Co., Ltd.	81
Energy Chemical	61
Sichuan Kulinan Technology Co., Ltd	57
Tianjin heowns Biochemical Technology Co., Ltd.	57
Dalian Meilun Biotech Co., Ltd.	58

View Lastest Price from LUCIGENIN manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2026-07-16	Lucigenin 2315-97-1	$29.00		99.42%	10g	TargetMol Chemicals Inc.
2025-06-07	N,N'-Dimethyl-9,9'-bisacridinium nitrate 2315-97-1		5g	>99.00%	5g	Xian confluore Biological Technology Co., Ltd.
2019-07-06	LUCIGENIN 2315-97-1	$1.00	1g	99%	100KG	Career Henan Chemical Co

Lucigenin
2315-97-1
$29.00
99.42%
TargetMol Chemicals Inc.

N,N'-Dimethyl-9,9'-bisacridinium nitrate
2315-97-1
$0.00
>99.00%
Xian confluore Biological Technology Co., Ltd.

LUCIGENIN
2315-97-1
$1.00
99%
Career Henan Chemical Co

LUCIGENIN Spectrum

LUCIGENIN(2315-97-1)MS LUCIGENIN(2315-97-1)¹HNMR LUCIGENIN(2315-97-1)IR1 LUCIGENIN(2315-97-1)IR2

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Lucigenin [bis-N-methylacridinium nitrate] LUCIGENIN(RG) LUCIGENIN(REAGENT / STANDARD GRADE) Lucigenin [Bis-N-methylacridinium nitrate] *UltraPure Grade*

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lucigenin or N,N′-dimethyl-9,9′-biacridinium dinitrate L-6868 NSC-151912 9,9'-BIS(N-METHYLACRIDINIUM NITRATE) LUCIGENIN 10,10'-Dimethyl-9,9'-biacridinium Dinitrate [for Chemiluminescence Research] BIS(N-METHYLACRIDINIUM) DINITRATE BIS(N-METHYLACRIDINIUM) NITRATE N,N'-DIMETHYL-9,9'-BIACRIDINIUM NITRATE N,N'-DIMETHYLBISACRIDINIUM DINITRATE 10,10’-dimethyl-9,9’-biacridinium N,N-dimethyl-9,9-bisacridinium nitrate N,N'-DIMETHYL-9,9'-BIACRIDINIUM- BIS-N-METHYLACRIDINIUM NITRATE 97% Lucigenin, HPLC 99% BIS(N-METHYLACRIDINUIM) DINATRATE 9,9μ-Bis(N-methylacridinium nitrate), Lucigenin 10,10'-Dimethyl-9,9'-biacridine-10,10'-diium·2nitrate Lucigenine 10,10'-Dimethyl-9,9'-biacridine-10,10'-diium 9,9'-Bi(10-methylacridinium) 9,9'-Bi[10-methylacridinium] 10,100-dimethyl-9,90-biacridinium Dinitrate N,n0-dimethyl-9,90-biacridinium Dinitrate 9,90biacridinium, 10,100-dimethyl-, Dinitrate 9,90-bis(nmethylacridinium Nitrate) Lucigenin Nitrate 10-methyl-9-(10-methylacridin-10-ium-9-yl)acridin-10-ium,dinitrate Bis(N-methylacridinium) Nitrate [for Chemiluminescence Research] N,N'-Dimethyl-9,9'-biacridinium dinitrate, ≥97.0% Bis(N-methylacridinium) Nitrate [for Chemiluminescence Research]> N,N'-Dimethyl-9,9'-biacridinium dinitrat LUCIGENIN USP/EP/BP 9,9'-Biacridinium, 10,10'-dimethyl-, nitrate (1:2) N,N′-Dimethyl-9,9′-biacridinium dinitrate, ≥98% N,N'-Dimethyl-9,9'-biacridinium dinitrate 10,10'-Dimethyl-9,9'-biacridinium Dinitrate Lucigenin, Superoxide anion radical chemiluminescent probe Lucigenin, 10 mM in DMSO N,N′-Dimethyl-9,9′-biacridinium dinitrate Lucigenin(L-6868 10,10'-Dimethyl-[9,9'-biacridine]-10,10'-diium nitrate , Lucigenin N,N'-Dimethyl-9,9'-biacridiniumdinitrate Lucigenin (10,10'-Dimethyl-9,9'-biacridinium dinitrate) 2315-97-1 C28H22N22NO3 C28H22N4O6 C28H222N2O3 BioChemical Biochemicals and Reagents Detection Fluorescent Probes, Labels, Particles and Stains Acridinium (Chemiluminescence) Chemiluminescence Miscellaneous Natural Products Aromatic Carboxylic Acids, Amides, Anilides, Anhydrides & Salts Acridinium (Chemiluminescence) Acridine