Cyanine7 NHS ester

Description

Amine reactive Cyanine7, near infrared fluorescent dye, an improved analog of Cy7®.

NIR fluorophores can be used to take advantage of near infrared window of biological tissues - increased transparency of tissues in this spectral region allows to carry out in vivo imaging.

This reagent can be utilized to produce Cyanine7-labeled biomolecules for subsequent use in various in vivo research, and drug design related experiments.

The structure of Cyanine7 features rigidized design of central polymethyne chain. This molecular reinforcement allows to increase quantum yield by 20% compared with parent structure, increasing fluorescence brightness.

This reagent requires organic co-solvent for the labeling (please see Recommended Protocols section below). Water-soluble Cyanine7 NHS ester is also available, and recommended for protein NIR labeling.

Chemical Properties

Appearance: Dark green solid
ex/em: 740/770 nm (MeOH/PBS)
Extinction coefficient (ε): 199000 Lmol1cm1
Quantum yield (Φ): 0.3

Abs/Em Maxima

750/773 nm

Fluoresene quantum yield

0.3

Extinction Coefficient

199000

CF260

0.022

CF280

0.029