Aminoacylase
- CAS No.
- 9012-37-7
- Chemical Name:
- Aminoacylase
- Synonyms
- ACYLASE;ACYLASE I;L-AMINOACYLASE;hippuricase;ACYLASE 1;histozyme;hippurase;acyclase I;EC 3.5.1.14;benzamidase
- CBNumber:
- CB3458624
- Molecular Formula:
- C30H34Cl2N4O
- Molecular Weight:
- 537.52316
- MDL Number:
- MFCD00081285
- MOL File:
- 9012-37-7.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Product description | Number | Pack Size | Price |
| Acylase I from Aspergillus melleus powder, brown, >0.5 U/mg | 01818 | 5g | $52.1 |
| Acylase I from Aspergillus melleus powder, brown, >0.5 U/mg | 01818 | 25g | $189 |
| Acylase I, immobilized on Eupergit® C from Aspergillus sp. ≥50?U/g moist material | 01824 | 1G | $126 |
| Acylase from Aspergillus genus | A0688 | 25g | $109 |
| A Cyclase I Purifiedbyimmunoaffinitychromatography. | 519637 | 100ul | $736 |
| More product size | |||
| storage temp. | 2-8°C |
|---|---|
| form | salt-free, lyophilized powder |
| color | yellow-brown |
| Specific Activity | 300-1,500units/mg protein |
| InChIKey | ULGJWNIHLSLQPZ-UHFFFAOYSA-N |
| SMILES | C(NCCC1C2=C(NC=1)C=CC=C2)(=O)CCCCCCNC1C2CCCCC=2N=C2C=1C(Cl)=CC(Cl)=C2 |
| EWG's Food Scores | 1 |
| EPA Substance Registry System | Aminoacylase (9012-37-7) |
| UNSPSC Code | 12352204 |
| NACRES | NA.54 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |   GHS07,GHS08 |
|---|---|
| Signal word | Danger |
| Hazard statements | H315-H319-H334-H335 |
| Precautionary statements | P261-P264-P271-P302+P352-P304+P340+P312-P305+P351+P338 |
| target organs | Respiratory system |
| PPE | Eyeshields, Gloves, type N95 (US) |
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24/25-36/37-26-24 |
| WGK Germany | 3 |
| RTECS | BF4890000 |
| F | 3-10-21 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Eye Irrit. 2 Resp. Sens. 1 Skin Irrit. 2 STOT SE 3 |
Aminoacylase price More Price(25)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 01818 | Acylase I from Aspergillus melleus powder, brown, >0.5 U/mg | 9012-37-7 | 5g | $52.1 | 2026-04-30 | Buy |
| Sigma-Aldrich | 01818 | Acylase I from Aspergillus melleus powder, brown, >0.5 U/mg | 9012-37-7 | 25g | $189 | 2026-04-30 | Buy |
| Sigma-Aldrich | 01824 | Acylase I, immobilized on Eupergit® C from Aspergillus sp. ≥50?U/g moist material | 9012-37-7 | 1G | $126 | 2023-01-07 | Buy |
| TCI Chemical | A0688 | Acylase from Aspergillus genus | 9012-37-7 | 25g | $109 | 2026-04-30 | Buy |
| Usbiological | 519637 | A Cyclase I Purifiedbyimmunoaffinitychromatography. | 100ul | $736 | 2026-06-03 | Buy |
Aminoacylase Chemical Properties,Uses,Production
Description
Aminoacylase-1 (EC 3.5.1.14) is a homodimeric zinc-binding metalloenzyme. A cytosolic enzyme with a wide range of tissue expression, it cleaves acylated L-amino acids (except L-aspartate) into L-amino acids and an acyl group. L-aspartate derivatives are cleaved by aminoacylase-2 (aspartoacylase). Aminoacylase-1 is the most abundant of the aminoacylases, a class of enzymes involved in hydrolysis of N-acetylated proteins.
Chemical Properties
Light red or light yellow lyophilized powder, soluble in water, optimum pH 7.0-7.5 (pig kidney, amylase) or 7-8 (mitragynase). Stability: pure enzyme can be stored for a long time after lyophilization without loss of vitality; crude enzyme solution can be stored in refrigerator for a short period of 1-2 days after adding toluene; crude enzyme preparation can be stabilized at 5℃; enzyme aqueous solution can keep the vitality unchanged for 60min at 70℃ when the pH is 7, and it is rapidly deactivated at 70℃ when the pH is less than 5. Requirements for the chemical structure of the substrate: single carboxy amino acid; amino acid α-carbon atom on the amino acid is acylated, acylation group of different hydrolysis speed; different sources of enzymes on the substrate chemical structure of the specificity is not consistent. Enzyme reaction: N-acylated-L-amino acid + H2O═ fatty acid anion + L-amino acid.
Uses
Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids .
Application
Acylase I from porcine kidney has been used to study the acylase I-catalyzed deacetylation of various S-alkyl-N-acetyl-L-cysteines and their carbon and oxygen analogues . Acylase I may be useful to catalyze N-acetyl amino acids to enantiomerically pure L-amino acids.
Biological Functions
Aminoacylases (N-acyl-L-amino acid amidohydrolases; EC 3.5.1.14) are widely found in animals, plants and microorganisms. The primary function of these enzymes is to remove acyl residues from N-acetylated amino acids although they may also be capable of hydrolysing carboxylic acid amides to fatty acid anions and L-amino acids. Although the catalytic mechanism of aminoacylases has been known for decades, the physiological role of these enzymes is still poorly understood. Activities of a similar nature, however, have been found in certain carboxypeptidases, aminopeptidases and dipeptidases. It could be therefore suggested that aminoacylases have a role to play in protein/peptide turnover.
General Description
Acylase I belongs to the aminoacylase family of enzymes.
Biochem/physiol Actions
Acylase I catalyzes the deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines. n-Butylmalonic acid is an inhibitor of acylase I. S-alkyl-N-acetyl-L-cysteines with short (C0-C3) and unbranched S-alkyl substituents have been found to be good acylase I substrates .
Enzyme inhibitor
Aminoacylase is a metallo-enzyme that needs Zinc (Zn2+) as a cofactor to function. The Zinc ions inside of aminoacylase are each coordinated to histidine, glutamate, aspartate, and water. The Zinc ion polarizes the water, facilitating its deprotonation by a nearby basic residue. The negatively charged hydroxide ion is nucleophilic and attacks the electrophilic carbonyl carbon of the substrate's acyl group.The exact mechanism after this point is unknown, with one possibility being that the carbonyl then reforms, breaks the amide bond, and forms the two products. At some point in the mechanism, another water molecule enters and coordinates with Zinc, returning the enzyme to its original state.
The nucleophilic attack by water is the rate-limiting step of aminoacylase's catalytic mechanism. This nucleophilic attack is reversible while the subsequent steps are fast and irreversible. This reaction sequence is an example of Michaelis–Menten kinetics, allowing one to determine KM, Kcat, Vmax, turnover number, and substrate specificity through classic Michaelis-Menten enzyme experiments. The second and third forward steps cause the formation and release of the reaction's products.
Aminoacylase Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29815 | 58 |
| BOC Sciences | +1-631-485-4226 | inquiry@bocsci.com | United States | 19936 | 58 |
| Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | sales@tnjchem.com | China | 34563 | 58 |
| Shanghai UCHEM Inc. | +862156762820 +86-13564624040 | sales@myuchem.com | China | 8799 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-029-89586680 +86-18192503167 | 1026@dideu.com | China | 9996 | 58 |
| Finetech Industry Limited | +86-27-8746-5837 +8619945049750 | info@finetechnology-ind.com | China | 9539 | 58 |
| Ningxia LabsChem Co., Ltd. | 0952-9294929 +8613895679378 | 48472788@qq.com | China | 352 | 58 |
| Shanghai Innopharm Industrial Co., Ltd. | +86-21-59960785 +86-17701845089 | sales@hoyaopharm.com | China | 338 | 58 |
| Hebei Fengjia New Material Co., Ltd | +86-0311-87836622 +86-18712993135 | sales01@tairunfaz.com | China | 8051 | 58 |
View Lastest Price from Aminoacylase manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2019-07-06 | AMINOACYLASE
9012-37-7
|
$8.00 | 1KG | 99% | 10tons | Career Henan Chemical Co |
-
- AMINOACYLASE
9012-37-7
- $8.00
- 99%
- Career Henan Chemical Co
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