CHK2 ANTIBODY

Source

Rabbit

Reactivity

Human;Mouse;Rat;Monkey

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues each followed by glutamine. These are known to be preferred sites for phosphorylation by ATM/ATR kinases. After DNA damage by ionizing radiation, UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain.

References

[1] Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
[2] Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
[3] Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
[4] Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
[5] Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
[6] Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
[7] Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
[8] Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

Raw materials

Preparation Products