CUT&RUN Assay Kit
- CAS No.
- Chemical Name:
- CUT&RUN Assay Kit
- Synonyms
- CUT&RUN Assay Kit
- CBNumber:
- CB36266751
- Molecular Formula:
- Molecular Weight:
- 0
- MDL Number:
- MOL File:
- Mol file
CUT&RUN Assay Kit Chemical Properties,Uses,Production
Description
The CUT&RUN Assay Kit is designed to conveniently provide reagents needed to perform up to 8 (P size) or 24 (S size) digestion reactions from cells and is optimized for 100,000 cells per reaction. The kit has been optimized to work for all types of DNA binding proteins, including histones, transcription factors and cofactors. A complete assay can be performed in as little as one dayThe CUT&RUN Assay Kit also provides important controls to ensure a successful CUT&RUN experiment. The kit contains a positive control Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb. PCR primer sets are provided for the human and mouse RPL30 gene locus to be used in conjunction with the control antibodies. This kit is compatible with both qPCR and NG-seq.
Background
Like the chromatin immunoprecipitation assay, Cleavage Under Targets & Release Using Nuclease is a powerful and versatile technique used for probing protein-DNA interactions within the natural chromatin context of the cell. This assay can be used to identify multiple proteins associated with a specific region of the genome, or the opposite, to identify the many regions of the genome associated with a particular protein. In addition, the CUT&RUN assay can be used to define the spatial and temporal relationship of a particular protein-DNA interaction. For example, the CUT&RUN assay can be used to determine the specific order of recruitment of various protein factors to a gene promoter or to “measure” the relative amount of a particular histone modification across an entire gene locus during gene activation. In addition to histone proteins, the CUT&RUN assay can also be used to analyze binding of transcription factors and cofactors, DNA replication factors, and DNA repair proteins.CUT&RUN provides a rapid, robust, and true low cell number assay for detection of protein-DNA interactions in the cell. Unlike the ChIP assay, CUT&RUN is free from formaldehyde cross-linking, chromatin fragmentation, and immunoprecipitation, making it a much faster and more efficient method for enriching protein-DNA interactions and identifying target genes. CUT&RUN can be performed in less than one day, from live cells to purified DNA, and has been shown to work with as few as 500-1000 cells per assay. Instead of fragmenting all of the cellular chromatin as done in ChIP, CUT&RUN utilizes an antibody-targeted digestion of chromatin, resulting in much lower signal than seen in the ChIP assay. As a result, CUT&RUN requires only 1/10th of the sequencing depth that is required for ChIP-seq assays. Finally, the inclusion of simple spike-in control DNA allows for accurate quantification and normalization of target-protein binding that is not possible with the ChIP method. This provides for effective normalization of signal between samples and between experiments.
References
[1] Skene, P.J. and Henikoff, S. (2017) Elife 6, pii: e21856. doi: 10.7554/eLife.21856.
[2] Skene, P.J. et al. (2018) Nat Protoc 13, 1006-19.
[3] Meers, M.P. et al. (2019) Elife 8, pii: e46314. doi: 10.7554/eLife.46314.
[4] Meers, M.P. et al. (2019) Mol Cell 75, 562-575.e5.
CUT&RUN Assay Kit Preparation Products And Raw materials
Raw materials
Preparation Products
CUT&RUN Assay Kit Suppliers
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai Universal Biotech Co.,Ltd | 15921930842 15921930842 | yh-wang@univ-bio.com | China | 25004 | 58 |
| Cell Signaling Technology Inc | 21-80243558 86218024 | info@cellsignal.cn | China | 6725 | 58 |
| Beijing Boning Biotechnology Co., Ltd. | 010-67500625 18210120938 | 384669408@qq.com | China | 195 | 58 |
| Supplier | Advantage |
|---|---|
| Shanghai Universal Biotech Co.,Ltd | 58 |
| Cell Signaling Technology Inc | 58 |
| Beijing Boning Biotechnology Co., Ltd. | 58 |
