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SEPHADEX G-50

CAS No.
9048-71-9
Chemical Name:
SEPHADEX G-50
Synonyms
SEPHADEX G-50 FINE;PDXTM G.F.;SEPHADEX G-50;Dextran gel G50;Sephadex G50, BR;SEPHADEX(R) G-50;PDX GF 50 (50-150);PXS-5505 free base;PDX GF 50 (100-300);SEPHADEX G-50 COARSE
CBNumber:
CB3728066
Molecular Formula:
NULL
Molecular Weight:
0
MDL Number:
MFCD00132771
MOL File:
Mol file
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-04-24 14:58:28

SEPHADEX G-50 Properties

form Solid
color White to off-white
Major Application life science and biopharma
CAS DataBase Reference 9048-71-9
UNSPSC Code 23151817
NACRES NA.56

SAFETY

Risk and Safety Statements

PPE Eyeshields, Gloves, type N95 (US)
WGK Germany  3
HS Code  39140000
Storage Class 11 - Combustible Solids

SEPHADEX G-50 price More Price(28)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich G5080 Sephadex? G-50 Fine 9048-71-9 50g $568 2026-04-30 Buy
Biosynth YS165316 Sephadex G50 9048-71-9 50g $324 2026-06-05 Buy
Biosynth YS165316 Sephadex G50 9048-71-9 100g $506 2026-06-05 Buy
Biosynth YS165316 Sephadex G50 9048-71-9 250g $1015 2026-06-05 Buy
Biosynth YS165316 Sephadex G50 9048-71-9 500g $1525 2026-06-05 Buy
Product number Packaging Price Buy
G5080 50g $568 Buy
YS165316 50g $324 Buy
YS165316 100g $506 Buy
YS165316 250g $1015 Buy
YS165316 500g $1525 Buy

SEPHADEX G-50 Chemical Properties, Uses, Production

Description

Sephadex G-50 Superfine is a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. The Superfine´s small bead size give higher efficiency.

  1. Quickly desalts, removes contaminants and transfers to a new buffer in a single step.
  2. Suitable for purification of DNA from small molecules by gel filtration
  3. Small bead size for shorter diffusion distances
  4. Smallest bead size in the Sephadex G-50 range
Sephadex is a gel filtration medium prepared by crosslinking dextran with epichlorohydrin. Different types of Sephadex differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. Sephadex G-50 is one of five different G-types ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 is available in 4 different particle sizes (Coarse, Medium, Fine & Superfine) and Superfine has the smallest bead size for higher efficiency with shorter diffusion distances. Coarse and Medium are are preferred for large scale group separations where high flow rates and low operating pressures are required.

Uses

The media used for gel exclusion chromatography include dextran (Sephadex™), polyacrylamide (Bio-Gel P™) and dextran-polyacrylamide (Sephacryl™) and agarose (Sepharose™ and BioGel A™). Each is available with a variety of different ranges of pore size in the beads, permitting separation of macromolecules of different size.
A gel with a smaller range of pore sizes (and hence a smaller range over which it can separate macromolecules on the basis of their size) will give a higher resolution; a gel with a wider range will give lower resolution, but will permit fractionation of a larger range of sizes as an initial step when the approximate molecular mass of the enzyme is not known.
The particle size of the gel beads (the mesh size) also affects resolution; smaller beads permit higher resolution, but a lower flow rate through the column (and hence a slower separation).
The table shows the useful range for the most commonly used gel filtration media - the lower and upper molecular sizes (in kDa) over which they can be used to separate macromolecules. The upper limit is known as the exclusion limit of the gel - the size above which proteins will elute in the void volume of the column.

 
lower limit
upper limit
dextran gels
Sephadex G-50
1.5
30
Sephadex G-75
3
80
Sephadex G-100
4
150
Sephadex G-150
5
300
Sephadex G-200
5
600
polyacrylamide gels
Bio-Gel P-10
1.5
20
Bio-Gel P-30
2.5
40
Bio-Gel P-60
3
60
Bio-Gel P-100
5
100
Bio-Gel P-150
15
150
BioGel P-200
30
200
Bio-Gel P-300
60
400
dextran-polyacrylamide gels
Sephacryl S-200
5
250
Sephacryl S-300
10
1500
Sephacryl S-400
20
8000
agarose gels
Sepharose 6B
10
4000
Sepharose 4B
60
20,000
Sepharose 2B
70
40,000
Bio-Gel A-0.5
10
500
Bio-Gel A-1.5
10
1500
Bio-Gel A-5
10
5000
Bio-Gel A-15
40
15,000
Bio-Gel A-50
100
50,000

Uses

Sephadex G-50 is a gel filtration medium used in affinity chromatography, protein chromatrography and gel filtration chromatography.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

SEPHADEX G-50 Preparation Products And Raw materials

Raw materials

Preparation Products

Global Suppliers ( 208)
Supplier Tel Email Country ProdList Advantage
Beijing Baileisen Biotechnology Co., Ltd. 13581502185 joe0415zhaxi@163.com China 141 58
Nanjing Dulai Biotechnology Co., Ltd. 025-84699383-8003;025-846993838003-8003 18013301590 njduly@126.com China 3293 55
Beijing RuiDaHengHui Science&Technology Development Co., LTD 010-64126590-807 13031133168 551502858@qq.com China 933 58
future industrial shanghai co., ltd 400-0066400 13621662912 sales@jonln.com China 1989 65
Beijing HwrkChemical Technology Co., Ltd 89508211 18501085097 sales.bj@hwrkchemical.com China 9409 55
Sichuan Kulinan Technology Co., Ltd 400-1166-196 18981987031 cdhxsj@163.com China 11674 57
Tianjin heowns Biochemical Technology Co., Ltd. 400 638 7771 sales@heowns.com China 14412 57
Sinopharm Chemical Reagent Co,Ltd. 86-21-63210123 sj_scrc@sinopharm.com China 9791 79
Hunan Hui Bai Shi Biotechnology Co., Ltd. 0731-85526065 13308475853 ivy@hnhbsj.com China 7231 62
ShangHai YuanYe Biotechnology Co., Ltd. 18916372613 shyysw007@163.com China 4993 60

View Latest Price from SEPHADEX G-50 manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
Sephadex G-50 pictures 2019-07-06 Sephadex G-50
9048-71-9
$1.00 1kg 95%-99% 1000kg Career Henan Chemical Co

SEPHADEX G-50 Spectrum

Sephadex G50, BR PDXTM G.F. Sephadex(R) G-50 Fine Sephadex G-50, Medium beads PDX GF 50 (100-300) PDX GF 50 (50-150) SEPHADEX G-50 SEPHADEX G-50 COARSE Sephadex G-50, Fine beads Cross-inked dextran gel G-50 Sephadex G-50 100-200 Sephadex G-50 200-400 Sephadex G-50 60-100 SEPHADEX G-50 MEDIUM SEPHADEX G-50 SUPERFINE SEPHADEX(R) G-50 sephadex(R) G-50 fine for chromato-graphy sephadex(R) G-50 medium for chromato-graphy sephadex(R) G-50 superfine for chromato-graphy Cross-linked dextran gel G-50 SEPHADEX(R) G-50 MEDIUM FOR CHROMATO- GRAPHY, 50-150 UM* SEPHADEX G-50, FOR GEL FILTRATION, 100-300MICRON SEPHADEX G-50 MEDIUM DNA GRADE SEPHADEX G-50, FOR GEL FILTRATION, 50-15 0MICRON SEPHADEX(R) G-50 FINE FOR CHROMATO- GRAPHY, 20-80 UM* SEPHADEX G-50, FOR GEL FILTRATION, 20-50MICRON D(+)-GLUCOSE MOLECULAR BIOLOGY REAGENT SEPHADEX(R) G-50 SUPERFINE FOR CHROMATO- GRAPHY, 20-50 UM* SEPHADEX G-50, FOR GEL FILTRATION, 20-80MICRON SEPHADEX G-50 FINE DNA GRADE SEPHADEX G-50, SUPERFINE 20-50MICRON SEPHADEX G-50, FINE 20-80MICRON SEPHADEX G-50, MEDIUM 50-150MICRON SEPHADEX G-50, COARSE 100-300MICRON Dextran gel G50 Sephadex G 50 (9CI, ACI) Sephadex G-50 (100-300um) Dextran Gel G-50 60-100 Sephadex? G-50 SEPHADEX G-50 FINE PXS-5505 free base 9048-71-9 DNA & RNA Purification Gel Filtration Media Gel Filtration Chromatography Sephadex Resins Resins and Media Reagents Protein Analysis Protein Chromatography Proteomics and Protein Expression Molecular Biology BioChemical Analytical Chromatography Product Catalog AMEKO Gel Filtration Media Sephadex G-50