Methylene Blue trihydrate
- CAS No.
- 7220-79-3
- Chemical Name:
- Methylene Blue trihydrate
- Synonyms
- METHYLENE BLUE HYDRATE;CI 52015;Methylthioninium Chloride Trihydrate;Methylthioninium;Methylene Blue, CP;METHYLTHIONINE CHLORIDE;METHYLENE BLUE CHLORIDE;METHYLENE BLUE INDICATOR;Methylene Blue trihydratee;Methyltltioninium Chloride
- CBNumber:
- CB3748860
- Molecular Formula:
- C16H20ClN3OS
- Molecular Weight:
- 337.87
- MDL Number:
- MFCD00150008
- MOL File:
- 7220-79-3.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 190 °C (dec.)(lit.) |
|---|---|
| Density | 0.98 g/mL at 25 °C |
| bulk density | 400-600kg/m3 |
| Flash point | 14 °C |
| storage temp. | no restrictions. |
| solubility | Soluble in water, chloroform; sparingly soluble in ethanol |
| form | Solid |
| pka | 2.6, 11.2(at 25℃) |
| Colour Index | 52015 |
| color | Green |
| PH | 3 (20°C, 10g/L in H2O) |
| Water Solubility | 4 g/100 mL |
| λmax | 668 nm, 609 nm |
| Merck | 13,6085 |
| Stability | Stable. Incompatible with bases, reducing agents, strong oxidizing agents. |
| Biological Applications | Diagnosis of tauopathy,Alzheimer’s disease (AD),Pick’s disease; treating prophylaxis; dental materials |
| InChI | 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1 |
| InChIKey | YAFGUSOAMIMROI-UHFFFAOYSA-M |
| SMILES | [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C |
| Toxicology and Carcinogenesis | Toxicology and Carcinogenesis Studies of Methylene Blue Trihydrate (CASRN 7220-79-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies) |
| CAS DataBase Reference | 7220-79-3(CAS DataBase Reference) |
| FDA UNII | T42P99266K |
| IARC | 3 (Vol. 108) 2016 |
| EPA Substance Registry System | Methylene blue trihydrate (7220-79-3) |
| UNSPSC Code | 41116130 |
| NACRES | NA.25 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|---|---|
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264-P270-P301+P312-P501 |
| Hazard Codes | Xn,F |
| Risk Statements | 22-36/37/38-11 |
| Safety Statements | 26-36-24/25-16-7 |
| RIDADR | UN 1170 3/PG 2 |
| WGK Germany | 3 |
| RTECS | SP5740000 |
| TSCA | Yes |
| HS Code | 32129000 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Acute Tox. 4 Oral |
Methylene Blue trihydrate price More Price(46)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 1.59270 | Methylene blue (C.I. 52015) Reag. Ph Eur | 7220-79-3 | 10g | $57.6 | 2026-04-30 | Buy |
| Sigma-Aldrich | 6210-OP | OmniPur Methylene Blue, Trihydrate - CAS 7220-79-3 - Calbiochem | 7220-79-3 | 100g | $181 | 2026-04-30 | Buy |
| Sigma-Aldrich | 1.59270 | Methylene blue (C.I. 52015) Reag. Ph Eur | 7220-79-3 | 100g | $212 | 2026-04-30 | Buy |
| Sigma-Aldrich | 1.15943 | Methylene blue (C.I.52015) for microscopy Certistain? | 7220-79-3 | 100g | $293 | 2023-06-20 | Buy |
| Sigma-Aldrich | 1.15943 | Methylene blue (C.I.52015) for microscopy Certistain? | 7220-79-3 | 25g | $68.9 | 2023-01-07 | Buy |
Methylene Blue trihydrate Chemical Properties, Uses, Production
Chemical Properties
Methylene Blue trihydrate is a dark green crystalline powder that has a bronze-like luster. When dissolved in water or alcohol, it creates a deep blue color. Methylene blue is used as a bacteriologic stain and as an indicator. It can also inhibit guanylate cyclase and has been used to treat cyanide poisoning and lower levels of methemoglobin.
Uses
Methylene Blue trihydrate finds many uses in biology and chemistry. It acts as a GCS inhibitor, biological stain and redox indicator. It inhibits tau filament formation. Further, it is reported to bind to the M2 subtype of muscarinic receptors in rat cardiac myocytes and thus exhibit antimuscarinic effects on IK, ACh and ICa. It also inhibits endothelium-dependent relaxation of isolated blood vessels.
Application
Methylene blue is not an officially restricted drug. It is mainly used to control water mildew and small melon insects. It can replace malachite green. Methylene blue dissolved in water does little harm to fish. In addition, the drug has made achievements in many fields such as industry, medicine and skin care. Even the drug will be applied to more difficult cancer diseases. Methylene blue is more effective in the treatment of non advanced fish diseases. Methylene blue is an oxidant, which can oxidize nitrite into nitrate, inhibit and kill bacteria. However, it also has a certain impact on nitrifying bacteria, so some measures should be taken after use.
Methylene blue can be used in the manufacture of ink and lakes and the dyeing of biological and bacterial tissues. It can be used for dyeing cotton, hemp, silk fabrics, paper and bamboo and wood. It can also be mixed with crystal violet and yellow dextrin in the ratio of 78:13:9 to form alkaline magenta blue.
Preparation
Methylene blue is synthesized commercially by oxidation of N,N-dimethyl-phenylenediamine with sodium dichromate (Na2Cr2O7) in the presence of sodium thiosulfate (Na2S2O3), followed by further oxidation in the presence of N,N-dimethylaniline (NTP, 2008). Methylene blue hydrochloride is isolated by addition of 30% hydrochloric acid and of a saturated common salt solution to the dye solution; after filtration, the product is washed with a 2% common salt solution. Instead of sodium dichromate, manganese dioxide, and catalytic amounts of copper sulfate can be used for the oxidation (Berneth, 2008).
Methylene blue of high purity can be obtained by chloroform extraction of impurities from solutions of raw dye in borate buffer at pH 9.5–10, followed by acidification of the aqueous solution and isolation of the dye (Berneth, 2008).
IARC Monographs
General Description
Methylene blue trihydrate appears as odorless or almost odorless dark green crystals with bronze luster or dark green crystalline powder. pH (1% solution in water) 3 to 4.5. (NTP, 1992)
Air & Water Reactions
Hygroscopic. Water soluble. Gives a blue solution in water.
Reactivity Profile
Methylene Blue trihydrate is incompatible with strong oxidizing agents, caustics, alkali metal iodides and reducing agents. Also incompatible with dichromates. Methylene Blue trihydrate forms double salts with many inorganic salts. Is bleached reversibly by (zinc + hydrochloric acid) or sodium hydrosulfite. In aqueous solution, Methylene Blue trihydrate is decolorized by zinc dust and dilute sulfuric acid, but the color is restored upon exposure to air and more rapidly upon addition of ammonium hydroxide.
Fire Hazard
Flash point data for Methylene Blue trihydrate are not available; however, Methylene Blue trihydrate is probably combustible.
storage
Room temperature
Methylene Blue trihydrate Preparation Products And Raw materials
Raw materials
Preparation Products
1of2
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Kaiyuan city Shenxin fine chemical co., ltd. | 024-22595298 18698863499 | 1253960@qq.com | China | 165 | 58 |
| Rhawn Reagent | 400-400-1332688 18019345275 | huyue@rhawn.cn | China | 14942 | 58 |
| SHENYANG SINOCHEM BIOTECH CO.,LTD | 23769576 13840269002 | 274311108@qq.com | China | 255 | 58 |
| Shenyang Shengyuan chemical industry co., ltd | 13190211560 | 1319074246@qq.com | China | 95 | 58 |
| BIOISCO(Lianyungang, Jiangsu) Biotechnology Co., Ltd | 18082049515 | 2757382053@qq.com | China | 2995 | 58 |
| Shenyang Delier Biotechnology Co., Ltd. | 15942301471 15942301471 | 15942301471@163.cn | China | 114 | 58 |
| Xiamen Weijian Yanfang Biotechnology Co., Ltd | 18020748636 13799798806 | sqx303@163.com | China | 20 | 58 |
| Guangzhou Viocon Pharmaceutical Technology Co., Ltd | +86-13424074264; 13726739909 | chenhuini@cti-cert.com | China | 497 | 58 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
Related articles
- Applications of Methylene Blue
- The chemical formula of methylene blue is C16H18ClN3S, with a relative molecular weight of 319.85.
- Apr 20,2022
View Latest Price from Methylene Blue trihydrate manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-18 | Methylene Blue trihydrate
7220-79-3
|
1KG | 98.5%min | 5000kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-09-18 | Methylene Blue trihydrate
7220-79-3
|
0.99 | RongNa Biotechnology Co.,Ltd | ||||
 |
2026-08-07 | Methylene Blue trihydrate
7220-79-3
|
$1.00-2.10 | 1KG | 99% | 20000KG | Shaanxi Dideu Medichem Co. Ltd |
-
- Methylene Blue trihydrate
7220-79-3
- 98.5%min
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Methylene Blue trihydrate
7220-79-3
- 0.99
- RongNa Biotechnology Co.,Ltd
-
- Methylene Blue trihydrate
7220-79-3
- $1.00-2.10
- 99%
- Shaanxi Dideu Medichem Co. Ltd
