DusQ 2 phosphoramidite

Description

DusQ^® 2 phosphoramidite is suitable for the synthesis of oligonucleotides labeled with DusQ 2 quencher at the 5′- and 3′-ends, as well as in the middle of the sequence. This phosphoramidite has DMT protection, which allows purification of the synthesized 5′-labeled oligonucleotide on cartridges.

This phosphoramidite is commonly used for the synthesis of doubly labeled probes for quantitative PCR with DusQ 2 quencher at the 5′-end. DusQ 2 is a fluorescence quencher with maximum absorption in the range of 560–670 nm and is suitable for efficient quenching of fluorophores emitting in this range through FRET mechanism. It is also used in hybridization probes based on static and mixed quenching.

Since the quenching efficiency of DusQ 2 is minimally dependent on the spectral overlap between the fluorophore and the quencher, it is suitable for a wide range of fluorophores, including those with emission in the red and far-red ranges. The list of fluorophores for use with DusQ 2 includes, but is not limited to, Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.

Coupling: 6 minutes coupling time recommended.

Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).

Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.

Uses

DMT-BH2 amidite (BHQ-2 DMT amidite) is a black quencher dye for the synthesis of dual-labeled oligonucleotide probes for qPCR bearing 5'-quencher. This quencher is ideal for HEX, JOE, ROX, Cyanine5, and other dyes with emissions in the orange and red parts of the spectrum.

Abs/Em Maxima

552 nm

CF260

0.31

CF280

0.26