Description
The Aurora kinases, Aurora A, -B, and -C, are a family of serine/threonine kinases encoded by distinct genes, AURKA, AURKB, and AURKC, respectively, that have roles in mitosis and meiosis.1,2 They are composed of an ATP-binding N-terminal domain, a highly conservated catalytic domain, and a short C-terminal domain that contains an activation loop and mediates cofactor binding.1 Each Aurora kinase contains a key threonine residue in its C-terminal activation loop, threonine 288 (Thr288), Thr232, and Thr198 in Aurora A, -B, and -C, respectively, that is autophosphorylated upon cofactor binding, inducing a conformational change that mediates kinase activation.1,2 Aurora A and Aurora B have roles in mitosis, whereas Aurora C regulates meiosis.2 Aurora A and Aurora B are constitutively expressed in mitotic cells, where Aurora A localizes to the spindle poles and Aurora B localizes to the central spindle.2 Aurora A has roles in mitotic entry, centrosome maturation and separation, and spindle assembly, whereas Aurora B regulates chromosome condensation and alignment, spindle assembly, and cytokinesis. Aurora C is expressed in germ cells during meiosis, localizes to the chromosome midbody, and is required for spermatogenesis.2,3 Aurora A, -B, and -C are overexpressed in a variety of cancers, including breast, colorectal, ovarian, and cervical cancers, and the pan-Aurora kinase inhibitor AMG 900 (Item No. 19176) inhibits tumor growth in an HCT116 mouse xenograft model.3,4 Cayman's Aurora A (Phospho-Thr288)/Aurora B (Phospho-Thr232)/Aurora C (Phospho-Thr198) Rabbit Monoclonal Antibody (Clone RM454) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.WARNING This product is not for human or veterinary use.
