ChemicalBook >> CAS DataBase List >>REVERSE TRANSCRIPTASE

REVERSE TRANSCRIPTASE

CAS No.
9068-38-6
Chemical Name:
REVERSE TRANSCRIPTASE
Synonyms
HIV-1 RT;abs60073;revertase;REVERSCRIPT 1;REVERSE TRANSCRIPTASE;HIV REVERSE TRANSCRIPTASE;AMV REVERSE TRANSCRIPTASE;REVERSE TRANSCRIPTASE AMV;RTL reverse transcriptase;M-MLV REVERSE TRANSCRIPTASE
CBNumber:
CB4702207
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00283754
MOL File:
Mol file
MSDS File:
SDS
TDS File:
TDS
Last updated:2026-05-28 01:27:07

REVERSE TRANSCRIPTASE Properties

storage temp. -20°C
form Liquid
color colorless
biological source Porcine intestinal mucosa
Specific Activity ≥10,000units/mL
≥5,000units/mg protein
UNSPSC Code 41105600
NACRES NA.55

SAFETY

Risk and Safety Statements

Symbol(GHS)  Health Hazard (GHS08)
GHS08
Signal word  Danger
Hazard statements  H334
Precautionary statements  P261-P284-P501
PPE Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
WGK Germany  3
HS Code  3504009000
Storage Class 12 - Non Combustible Liquids
Hazard Classifications Resp. Sens. 1

REVERSE TRANSCRIPTASE price More Price(10)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich M1302 M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis 9068-38-6 40000units $438 2026-04-30 Buy
ChemScene CS-0129882 M-MLV Reverse transcriptase 9068-38-6 1KU $42 2026-06-04 Buy
ChemScene CS-0129882 M-MLV Reverse transcriptase 9068-38-6 5KU $92 2026-06-04 Buy
ChemScene CS-0129882 M-MLV Reverse transcriptase 9068-38-6 10KU $139 2026-06-04 Buy
Apolloscientific BITP3203 rMMLV-RT 9068-38-6 2,500U $86 2026-06-04 Buy
Product number Packaging Price Buy
M1302 40000units $438 Buy
CS-0129882 1KU $42 Buy
CS-0129882 5KU $92 Buy
CS-0129882 10KU $139 Buy
BITP3203 2,500U $86 Buy

REVERSE TRANSCRIPTASE Chemical Properties, Uses, Production

Description

Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.

Uses

Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer

Uses

M-MLV Reverse Transcriptase has been used:

  • for the preparation of cDNA libraries or for first strand cDNA synthesis
  • for use in a 2-step RT-PCR assay
  • for the synthesis of cDNA that is further used in cloning

Uses

Reverse Transcriptase AMV is suitable for:

  • First- and second-strand cDNA synthesis and synthesis of first strand cDNA for use in subsequent amplification reactions (RT-PCR)
  • Dideoxy DNA sequencing
  • Primer extension
  • RNA sequencing
  • 3′-end labeling of DNA fragments
  • Generation of single-stranded probes for genomic footprint experiments

General Description

M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Purification Methods

This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]

REVERSE TRANSCRIPTASE Preparation Products And Raw materials

Raw materials

Preparation Products

Global Suppliers ( 203)
Supplier Tel Email Country ProdList Advantage
J & K SCIENTIFIC LTD. 18210857532 18210857532 jkinfo@jkchemical.com China 96815 76
Sichuan Kulinan Technology Co., Ltd 400-1166-196 18981987031 cdhxsj@163.com China 11674 57
Chengdu Ai Keda Chemical Technology Co., Ltd. 4008-755-333 18080918076 800078821@qq.com China 9706 55
Shanghai Aladdin Bio-Chem Technology Co.,LTD 400-6206333 13167063860 anhua.mao@aladdin-e.com China 29977 65
Nanjing Dulai Biotechnology Co., Ltd. 025-84699383-8003;025-846993838003-8003 18013301590 njduly@126.com China 3293 55
Shanghai BeiZhuo Biotech Co., Ltd. 021-61119791,13386096464 bzswkf@foxmail.com China 3916 50
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51389 80
Shanghai YuanYe Biotechnology Co., Ltd. 15026964105 2881489226@qq.com China 89667 60
Raw material medicin reagent co.,Ltd sales@njromanme.com China 4511 58
Guangzhou LES biological Technology Co.,Ltd. 13672434928;13672434928 3307355104@qq.com China 3647 58

View Latest Price from REVERSE TRANSCRIPTASE manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ pictures 2020-01-10 REVERSE TRANSCRIPTASE, M-MLV, RNASEH+
9068-38-6
$1.00 1KG 99% 200kg Career Henan Chemical Co
Revertase, RNA-directed DNA polymerase M-MLV REVERSE TRANSCRIPTASE MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE Nucleotidyltransferase, deoxyribonucleate, RNA-dependent REVERSE TRANSCRIPTASE, FOR MOL. BIOLOGY, DRY ICE, 500 U* REVERSE TRANSCRIPTASE FROM AVIAN*MYELOBL ASTOSIS VIR CYSCRIBE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, REC., INDUSTRIAL GMP GRADE amv-reverse transcriptase from avian myeloblastosis virus revertase Reverse Trancscriptase, Molony Mouse Leukemia Virus, Recombinant AVIAN MYELOBLASTOSIS VIRUS REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE HIV REVERSE TRANSCRIPTASE, RECOMBINANT, E COLI HIV-1 REVERSE TRANSCRIPTASE HIV-1 RT AMV REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE (HC) RNA-DIRECTED DNA POLYMERASE REVERSCRIPT 1 REVERSE TRANSCRIPTASE REVERSE TRANSCRIPTASE AMV REVERSE TRANSCRIPTASE, AMV, (HC) REVERSE TRANSCRIPTASE, M-MLV, RNASEH+ M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS M-MLV REVERSE TRANSCRIPTASE, RNASE H MINUS, POINT MUTANT Reverse Transcriptase from HIV, Recombinant Native Avian myeloblastosis virus AMV-Reverse Transcriptase ToloScript Reverse Transcriptase abs60073 M-MLV Reverse transcriptase BR RTL reverse transcriptase M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis Reverse Transcr?ptase(MMLV) Reverse Transcriptase, Recombinant, HIV, concentrate REVERSE TRANSCRIPTASE, M-MLV, RSEH+ 9068-38-6 Nucleic Acid Amplification Molecular Biology PCR/Amplification Plant Biotechnology Plant Molecular Biology RT-PCR BioChemical Enzyme 2.7.x.x Phosphorus containing groupsMore...Close... AmplificationMolecular Biology cDNA LabelingApplication Index PCRPlant Molecular Biology RT-PCREnzyme Class Index 2.x.x.x Transferases Functional Profiling of Stem Cells Gene Expression&Analysis Molecular Biology Tested Nucleic Acid Amplification PCR/Amplification RT-PCRMolecular Biology Stem Cell Biology