ChemicalBook >> CAS DataBase List >>SP6 RNA POLYMERASE

SP6 RNA POLYMERASE

CAS No.
Chemical Name:
SP6 RNA POLYMERASE
Synonyms
SP6 RNA;SP6 R POLYMERASE;SP6 RNA POLYMERASE
CBNumber:
CB4778598
Molecular Formula:
Molecular Weight:
0
MDL Number:
MFCD00212692
MOL File:
Mol file
Last updated:2025-01-27 09:38:02

SP6 RNA POLYMERASE Properties

storage temp. -20°C
form solution
color colorless
PH ~7.9 (39°F)
biological source Escherichia coli (BL 21/pSR3)
Water Solubility water: miscible
Specific Activity ≥20U/μL
UNSPSC Code 12352204

SP6 RNA POLYMERASE price More Price(5)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich RPOLSP6-RO SP6 RNA Polymerase from Escherichia coli BL 21/pSR3 1000units $204 2026-04-30 Buy
Sigma-Aldrich RPOLSP6-RO SP6 RNA Polymerase from Escherichia coli BL 21/pSR3 5000units $780 2026-04-30 Buy
ApexBio Technology K1096 SP6 RNA Polymerase 2000U $55 2026-05-06 Buy
ApexBio Technology K1096 SP6 RNA Polymerase 5000U $70 2026-05-06 Buy
ApexBio Technology K1096 SP6 RNA Polymerase 10000U $110 2026-05-06 Buy
Product number Packaging Price Buy
RPOLSP6-RO 1000units $204 Buy
RPOLSP6-RO 5000units $780 Buy
K1096 2000U $55 Buy
K1096 5000U $70 Buy
K1096 10000U $110 Buy

SP6 RNA POLYMERASE Chemical Properties, Uses, Production

Uses

SP6 RNA Polymerase can transcribe RNA from cloned DNA templates that are downstream from an SP6 promoter. The synthesis can be performed with labeled NTPs to generate highly labeled RNA. Synthesized RNA can be used in many applications, including:

  • RNA or DNA blotting techniques
  • In situ hybridization
  • RNase protection studies: Transcripts synthesized by the enzyme are used as precursor RNA for studies on RNA splicing and processing.
  • Synthesis of capped RNA in vitro with addition of m7GpppG or m7GpppA in excess over GTP or ATP during the transcription reaction. The generated antisense RNA can be introduced into cells to suppress the expression of the corresponding genes.
  • The synthesis of antisense RNA probes

General Description

With this system, homogeneously labeled single-stranded RNA can be generated. Transcripts can be non-radioactively labeled with biotin or DIG-11-UTP or radioactively labeled to high specific activity with [α-32P] or [α-35S] labeled nucleotides.

Contents:

  • SP6 RNA Polymerase, ≥20 U/μl, in buffer, pH 7.9
  • Transcription Buffer, 10x concentrated

SP6 RNA POLYMERASE Preparation Products And Raw materials

Raw materials

Preparation Products

SP6 RNA POLYMERASE Suppliers

Global Suppliers ( 136)
Supplier Tel Email Country ProdList Advantage
Sigma-Aldrich 021-61415566 800-8193336 orderCN@merckgroup.com China 51389 80
Shanghai YuanYe Biotechnology Co., Ltd. 15026964105 2881489226@qq.com China 89667 60
Guangzhou LES biological Technology Co.,Ltd. 13672434928;13672434928 3307355104@qq.com China 3647 58
Zhejiang Lianshuo Biotechnology Co., Ltd. 18616526224 lianshuo@vip.126.com China 9605 58
Shanghai jingkang bioengineering co., ltd. 021-54721350 13524668266; 2881505714@qq.com China 7866 58
Shanghai Kanglang Biotechnology Co., Ltd. 021-61998208 18217752821; sales@klbio.cn China 9619 58
Shanghai Sig Biotechnology Co., LTD 021-57810052 18930344717 2089316240@qq.com China 9390 58
Zhejiang Huida Biotech Co., LTD 0571-89903022 18679456698 zilong@hizyme.com China 8001 58
Aleshan (Guangzhou) Biotechnology Co., Ltd 18636736628 Araratbio@163.com China 3341 58
Nantong Feiyu Biological Technology CO.,LTD 0513-68819626 15152877458 feiyubio@163.com China 4807 58
SP6 RNA POLYMERASE SP6 RNA SP6 R POLYMERASE