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Biocytin

Biocytin structure

CAS No.: 576-19-2

Chemical Name:: Biocytin

Synonyms: BCT;BIOCYTIN;L-BIOCYTIN;d-Biocytin;H-LYS(BIOT)-OH;H-Lys(biotinyl);H-LYS(BIOTINYL)-OH;BIOCYTIN FREE BASE;Biocytin (εL-Lys(biotinyl)-OH

CBNumber:: CB5184561

Molecular Formula:: C16H28N4O4S

Molecular Weight:: 372.48

MDL Number:: MFCD00077319

MOL File:: 576-19-2.mol

MSDS File:: SDS

Last updated:2025-10-09 14:55:14

Product description	Number	Pack Size	Price
Biocytin ≥98% (TLC)	B4261	5mg	$55.42
Biocytin ≥98% (TLC)	B4261	25mg	$101.92
Biocytin ≥98%	16751	10mg	$37
Biocytin ≥98%	16751	25mg	$72
Biocytin ≥98% (TLC)	B4261	50mg	$164
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Biocytin Properties

Melting point	~245 °C (dec.) (lit.)
alpha	D25 +53° (c = 1.05 in 0.1N NaOH)
Boiling point	748.0±60.0 °C(Predicted)
Density	1.234
storage temp.	-20°C
solubility	Aqueous Base (Slightly), Water (Slightly, Sonicated)
form	Solid
pka	2.53±0.24(Predicted)
color	White to Off-White
Appearance	White to off-white powder
optical activity	[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)
Water Solubility	Soluble in water (50 mM)
Merck	13,1227
BRN	97197
InChIKey	BAQMYDQNMFBZNA-MNXVOIDGSA-N
SMILES	C(O)(=O)[C@H](CCCCNC(=O)CCCC[C@H]1[C@@]2([H])NC(=O)N[C@@]2([H])CS1)N
FDA UNII	G6D6147J22
UNSPSC Code	12352209
NACRES	NA.26

SAFETY

Risk and Safety Statements

WGK Germany	3
HS Code	29362900

Biocytin price More Price(33)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	B4261	Biocytin ≥98% (TLC)	576-19-2	5mg	$55.42	2025-07-31	Buy
Sigma-Aldrich	B4261	Biocytin ≥98% (TLC)	576-19-2	25mg	$101.92	2025-07-31	Buy
Cayman Chemical	16751	Biocytin ≥98%	576-19-2	10mg	$37	2024-03-01	Buy
Cayman Chemical	16751	Biocytin ≥98%	576-19-2	25mg	$72	2024-03-01	Buy
Sigma-Aldrich	B4261	Biocytin ≥98% (TLC)	576-19-2	50mg	$164	2025-07-31	Buy

Product number	Packaging	Price	Buy
B4261	5mg	$55.42	Buy
B4261	25mg	$101.92	Buy
16751	10mg	$37	Buy
16751	25mg	$72	Buy
B4261	50mg	$164	Buy

Biocytin Chemical Properties,Uses,Production

Description

Biocytin is a conjugate of biotin and lysine known formally as ε-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. For this and other applications, biocytin is detected with avidin or streptavidin probes.

Chemical Properties

White Solid

Uses

Useful for the synthesis of Biocytin containing peptides to study the mechanism of reactions catalyzed by biotin - containing enzymes

Uses

Biocytin can be used as a versatile fluorescent marker for neuroanatomical investigations and for a biotinidase assay.

Uses

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Definition

ChEBI: A monocarboxylic acid amide that results from the formal condensation of the carboxylic acid group of biotin with the N6-amino group of L-lysine.

Biological Activity

Versatile marker used in anterograde, retrograde and intracellular neuroanatomical investigations and in biotinidase assays. Displays high solubility in aqueous solutions and has a low molecular weight facilitating injection using micropipettes. Can be incorporated with a variety of avidin and streptavidin conjugates for detection by light, fluorescence or electron microscope.

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.

storage

-20°C

Purification Methods

Recrystallise biocytin rapidly from dilute MeOH or Me2CO. It can also be recrystallised from H2O by slow evaporation or by dissolving in the minimum volume of H2O and adding Me2CO until solid separates. It is freely soluble in H2O and AcOH but insoluble in Me2CO. [Wolf et al. J Am Chem Soc 74 2002 1952, 72 1048 1950.] It has been purified by chromatography on superfiltrol-Celite, Al2O3 and by countercurrent distribution and then recrystallised [IR: Peck et al. J Am Chem Soc 74 1991 1952]. The hydrochloride recrystallises from aqueous Me2CO/HCl and has m 227o (dec). [Beilstein 27 III/IV 7984.]

References

[1] MARGARET A. MACNEIL Paulette A G. Biocytin wide-field bipolar cells in rabbit retina selectively contact blue cones[J]. Journal of Comparative Neurology, 2007, 506 2: spc1. DOI: 10.1002/cne.21590
[2] E. FRIAUF C S S Mcconnell. Functional synaptic circuits in the subplate during fetal and early postnatal development of cat visual cortex[J]. Journal of Neuroscience, 1990, 15 1: 2601-2613. DOI: 10.1523/jneurosci.10-08-02601.1990
[3] RITA BOPP. Pyramidal cells make specific connections onto smooth (GABAergic) neurons in mouse visual cortex.[J]. PLoS Biology, 2014, 12 8: e1001932. DOI: 10.1371/journal.pbio.1001932

Biocytin Preparation Products And Raw materials

Raw materials

Preparation Products

Biocytin Suppliers

Global( 221)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
ATK CHEMICAL COMPANY LIMITED	+undefined-21-51877795	ivan@atkchemical.com	China	33024	60
GenicBio Limited	+86-21-37621270	service@genicbio.com	CHINA	991	58
Hubei xin bonus chemical co. LTD	86-13657291602	linda@hubeijusheng.com	CHINA	22963	58
BOC Sciences	+1-631-485-4226	inquiry@bocsci.com	United States	19552	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49975	58
career henan chemical co	+86-0371-86658258 +8613203830695	factory@coreychem.com	China	29799	58
Shaanxi Dideu Medichem Co. Ltd	+86-29-87569266 +86-17392581500	1015@dideu.com	China	3965	58
Guangzhou Yuheng Pharmaceutical Technology Co., Ltd	+8613580539051	joe@yuhengpharm.com	CHINA	21142	58
TargetMol Chemicals Inc.	+1-781-999-5354; +17819995354	marketing@targetmol.com	United States	32434	58
Career Henan Chemica Co	+86-0371-86658258 +8613203830695	laboratory@coreychem.com	China	30229	58

Supplier	Advantage
ATK CHEMICAL COMPANY LIMITED	60
GenicBio Limited	58
Hubei xin bonus chemical co. LTD	58
BOC Sciences	58
CONIER CHEM AND PHARMA LIMITED	58
career henan chemical co	58
Shaanxi Dideu Medichem Co. Ltd	58
Guangzhou Yuheng Pharmaceutical Technology Co., Ltd	58
TargetMol Chemicals Inc.	58
Career Henan Chemica Co	58

View Lastest Price from Biocytin manufacturers

Update time	Product	Price	Min. Order	Purity	Supply Ability	Manufacturer
2025-10-09	Biocytin 576-19-2	US $48.00-89.00 / mg		97.23%	10g	TargetMol Chemicals Inc.
2025-06-07	Biocytin 576-19-2	US $0.00 / g	1g	>95.00%	1g	Xian confluore Biological Technology Co., Ltd.
2022-02-19	H-Lys(biotinyl)-OH 576-19-2	US $0.00 / KG	1KG	98.9%	100 tons	Honest Joy Holdings Limited

Biocytin
576-19-2
US $48.00-89.00 / mg
97.23%
TargetMol Chemicals Inc.

Biocytin
576-19-2
US $0.00 / g
>95.00%
Xian confluore Biological Technology Co., Ltd.

H-Lys(biotinyl)-OH
576-19-2
US $0.00 / KG
98.9%
Honest Joy Holdings Limited

Biocytin Spectrum

Biocytin(576-19-2)MS

576-19-2(Biocytin)Related Search:

Imidazole D-Biotin 5-Hydroxytryptophan (S)-2-amino-3-(4-(2-tert-butoxy-2-oxoethyl)phenyl)propanoic acid H-LYS(ALLOC)-OH

H-L-Lys-NEt2.2HCl N-EPSILON-ACETYL-L-LYSINE H-Lys(Boc)-OBzl·HCl H-Lys(Boc)-OH H-L-Pen-OH.HCl.H2O

H-L-Tyr-NEt2.HCl L-Phenylalanine, 4-cyclohexyl- FMOC-BCT-OH,N-ALPHA-(9-FLUORENYLMETHOXYCARBONYL)-L-BIOCYTIN,FMOC BIOCYTIN N-(3-MALEIMIDOPROPIONYL)BIOCYTIN,N-(3-MALEIMIDOPROPIONYL)BIOCYTIN 65+%,3-(N-MALEIMIDOPROPIONYL)BIOCYTIN BCT

N-ALPHA-(9-FLUORENYLMETHOXYCARBONYL)-D-BIOCYTIN,FMOC-D-BCT-OH BOC-BCT-OH,nα-boc-biocytin,N-ALPHA-BOC-BIOCYTIN,N-ALPHA-T-BUTOXYCARBONYL-L-BIOCYTIN,N-T-BOC-BIOCYTIN,BOC-BIOCYTIN 3-(N-MALEIMIDYLPROPIONYL)BIOCYTIN

1of4

NE-BIOTYNYL-L-LYSINE N-EPSILON-BIOTIN-L-LYSINE N-EPSILON-(+)-BIOTINYL-L-LYSINE N-EPSILON-BIOTINYL-L-LYSINE N-EPSILON-D-BIOTINYL-L-LYSINE LYSINE(BIOTINYL)-OH L-BIOCYTIN EPSILON-BIOTINOYL-L-LYSINE H-LYS(BIOTINYL)-OH H-LYS(BIOT)-OH BIOCYTIN BCT BIOCYTIN FREE BASE (2S)-2-amino-6-[5-[(1S,2S,5R)-7-oxo-3-thia-6,8-diazabicyclo[3.3.0]oct-2-yl]pentanoylamino]hexanoic acid N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine (S)-2-aMino-6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]iMidazol-4-yl)pentanaMido)hexanoic acid Biocytin (ε-Biotinoyl-L-lysine) Bct, Nε-(+)-Biotinyl-L-lysine d-Biocytin N6-[5-[(3aS,4S,6aR)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-yl]-1-oxopentyl]-L-lysine N6-[5-[(3aS,6aβ)-Hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4α-yl]-1-oxopentyl]-L-lysine (2S)-6-[5-[(3aR,6S,6aS)-2-keto-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-amino-hexanoic acid (2S)-6-[5-[(3aR,6S,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-aminohexanoic acid (2S)-6-[5-[(3aR,6S,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-6-yl]pentanoylamino]-2-azanyl-hexanoic acid Biocytin 〔Nε-Biotinyl-L-lysine〕 Biocytin (&epsilon -Biotinoyl-L-lysine) H-Lys(biotinyl) L-Lys(biotinyl)-OH Nε-Biotinyl-L-lysine≥ 95% (NMR) Anti-BTD antibody produced in goat OTTHUMP00000160300 OTTHUMP00000209134 OTTHUMP00000209135 OTTHUMP00000209138 Biocytin *CAS 576-19-2* (2S)-6-{5-[(3aS,4S,6aR)-2-oxo-hexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamido}-2-aminohexanoic acid L-Lysine, N6-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]- Biocytin USP/EP/BP (2s)-6-[5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-2-aminohexanoic Acid inhibit,Endogenous Metabolite,Biocytin,(+)-Biocytin,Inhibitor Biocytin [Epsilon-Biotinoyl-L-Lysine] - 100 mg 576-19-2 C16H28N4O4S Substrates by Enzyme Modified Amino Acids Enzymes, Inhibitors, and Substrates A - H Enzyme Substrates Biotinidase Biochemicals and Reagents Amino Acids and Peptides BioChemical Amino Acids & Derivatives Biotin Derivatives