Fluorescein diacetate
- CAS No.
- 596-09-8
- Chemical Name:
- Fluorescein diacetate
- Synonyms
- FDA;Mfcd5062;Nsc 4726;Nsc 667259;Diacetylfluor;-Cellstain-Fda;fluoresceinacetate;DIACETYLFLUORESCEIN;3,6-DIACETOXYFLUORAN;Flomoxef Impurity 45
- CBNumber:
- CB5305799
- Molecular Formula:
- C24H16O7
- Molecular Weight:
- 416.38
- MDL Number:
- MFCD00005062
- MOL File:
- 596-09-8.mol
| Product description | Number | Pack Size | Price |
| Diacetylfluorescein European Pharmacopoeia (EP) Reference Standard | Y0000454 | 120 mg | $156 |
| Fluorescein Diacetate - CAS 596-09-8 - Calbiochem | 343209 | 5G | $114 |
| Diacetylfluorescein United States Pharmacopeia (USP) Reference Standard | 1182000 | 200mg | $398 |
| Fluorescein Diacetate >98.0%(HPLC) | F0240 | 5g | $80 |
| Fluorescein diacetate used as cell viability stain | F7378 | 5g | $90.7 |
| More product size | |||
| Melting point | 200-203 °C(lit.) |
|---|---|
| Boiling point | 494.73°C (rough estimate) |
| Density | 1.2738 (rough estimate) |
| refractive index | 1.6810 (estimate) |
| Flash point | 264° |
| storage temp. | −20°C |
| solubility | acetone: soluble25mg/mL |
| form | Crystalline Powder |
| pka | 6.4(at 25℃) |
| color | Light yellow |
| PH Range | Weak green uorescence (6.0) to strong green uorescence (7.2) |
| Water Solubility | Soluble in chloroform, DMSO, ethanol, and water. |
| ε(extinction coefficient) | ≥86000 at 488-494nm in 0.1 M NaOH |
| λmax | 490nm |
| BRN | 62575 |
| Major Application | LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia |
| Biological Applications | Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease |
| CAS DataBase Reference | 596-09-8(CAS DataBase Reference) |
| NCI Dictionary of Cancer Terms | FDA |
| FDA UNII | YL39R93PRE |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8) |
| UNSPSC Code | 41116107 |
| NACRES | NA.77 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319 | |||||||||
| Precautionary statements | P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313 | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 24/25-36/37/39-27-26 | |||||||||
| WGK Germany | 3 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 32129000 | |||||||||
| NFPA 704 |
|
Fluorescein diacetate price More Price(28)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | Y0000454 | Diacetylfluorescein European Pharmacopoeia (EP) Reference Standard | 596-09-8 | 120 mg | $156 | 2025-07-31 | Buy |
| Sigma-Aldrich | 343209 | Fluorescein Diacetate - CAS 596-09-8 - Calbiochem | 596-09-8 | 5G | $114 | 2025-07-31 | Buy |
| Sigma-Aldrich | 1182000 | Diacetylfluorescein United States Pharmacopeia (USP) Reference Standard | 596-09-8 | 200mg | $398 | 2025-07-31 | Buy |
| TCI Chemical | F0240 | Fluorescein Diacetate >98.0%(HPLC) | 596-09-8 | 5g | $80 | 2025-07-31 | Buy |
| Sigma-Aldrich | F7378 | Fluorescein diacetate used as cell viability stain | 596-09-8 | 5g | $90.7 | 2025-07-31 | Buy |
Fluorescein diacetate Chemical Properties,Uses,Production
Description
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.
Chemical Properties
Light yellow powder
Uses
Fluorescein diacetate is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. It has been used for the differentiation of live cells from dead cells. It can be also used as a fluorogenic substrate for hGSTP1-1.
Application
Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.
Biotechnological Applications
Fluorescein Diacetate Stain is an esterase substrate that can permeate cell membranes. In viable cells with an active metabolism, intracellular esterases hydrolyze Fluorescein Diacetate Stain to produce the green fluorescent compound fluorescein. Fluorescein accumulates in viable cells, causing them to fluoresce green.
Fluorescein Diacetate Stain can be used with Propidium Iodide Stain for Yeast to assess cell viability with the automated fluorescence cell counters of the LUNA® family. Viable cells convert fluorescein diacetate into fluorescein and thus fluoresce green. Nonviable cells take up propidium iodide and fluoresce red.
Fluorescence Signal Enhancer 1 can be used with Fluorescein Diacetate Stain to inhibit ATP-dependent fluorescein efflux from metabolically active cells. This will result in lower background and increased signal intensity, thereby enhancing the fluorescein signal-to-noise ratio.
Fluorescein diacetate Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29852 | 58 |
| BOC Sciences | +1-631-485-4226 | inquiry@bocsci.com | United States | 19552 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49975 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-29-87569266 +86-17392581500 | 1015@dideu.com | China | 3965 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354; +17819995354 | marketing@targetmol.com | United States | 32434 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 | sales@tnjchem.com | China | 34563 | 58 |
| ANHUI WITOP BIOTECH CO., LTD | +8615255079626 | eric@witopchemical.com | China | 23541 | 58 |
| Dayang Chem (Hangzhou) Co.,Ltd. | +86-0571-88938639 +8617705817739 | info@dycnchem.com | China | 52846 | 58 |
| changzhou huayang technology co., ltd | +8615250961469 | 2571773637@qq.com | China | 9644 | 58 |
| Nanjing Duly Biotech Co.,Ltd | +undefined18013301590 | doris@njduly.com | China | 2448 | 58 |
View Lastest Price from Fluorescein diacetate manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2025-09-11 | Fluorescein diacetate
596-09-8
|
US $42.00 / mg | 99.61% | 10g | TargetMol Chemicals Inc. | ||
 |
2019-07-06 | FLUORESCEIN DIACETATE
596-09-8
|
US $8.00 / kg | 1kg | 99% | 10MT | Career Henan Chemical Co |
-
- Fluorescein diacetate
596-09-8
- US $42.00 / mg
- 99.61%
- TargetMol Chemicals Inc.
-
- FLUORESCEIN DIACETATE
596-09-8
- US $8.00 / kg
- 99%
- Career Henan Chemical Co
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