Solvent Black 3
- CAS No.
- 4197-25-5
- Chemical Name:
- Solvent Black 3
- Synonyms
- SUDAN BLACK B;CI 26150;Nsc 11239;Black 2B;sot-black6;BSudan Bla;Sudanblack;199664-25G;CI NO 26150;typogenblack
- CBNumber:
- CB7198632
- Molecular Formula:
- C29H24N6
- Molecular Weight:
- 456.54
- MDL Number:
- MFCD00006919
- MOL File:
- 4197-25-5.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 120-124 °C(lit.) |
|---|---|
| Boiling point | 552.68°C (rough estimate) |
| Density | 1.4899 (rough estimate) |
| refractive index | 1.4570 (estimate) |
| storage temp. | Store at RT. |
| solubility | Insoluble in water; soluble in ethanol, acetone, benzene, toluene, xylene, ethylene glycol |
| pka | 2.94±0.40(Predicted) |
| Colour Index | 26150 |
| form | Powder/Solid |
| color | Very dark brown to black |
| Water Solubility | Soluble in oils, fats, warm petrolatum, paraffin, phenol, ethanol, acetone, benzene, toluene and hydrocarbon. Insoluble in water. |
| λmax | 598 nm, 415 nm |
| ε(extinction coefficient) | ≥20000 at 596-603nm in ethanol |
| Merck | 13,8970 |
| BRN | 723248 |
| Stability | Light Sensitive |
| Biological Applications | Diagnosis of acute myeloid leukemia (AML); detecting neuron-specific nuclear protein NeuN; drug screening |
| Major Application |
diagnostic assay manufacturing hematology histology |
| Cosmetics Ingredients Functions | HAIR DYEING |
| InChI | 1S/C29H24N6/c1-29(2)30-26-14-8-13-22-25(17-18-27(31-29)28(22)26)35-34-24-16-15-23(20-11-6-7-12-21(20)24)33-32-19-9-4-3-5-10-19/h3-18,30-31H,1-2H3/b33-32+,35-34+ |
| InChIKey | YCUVUDODLRLVIC-VPHDGDOJSA-N |
| SMILES | CC1(C)Nc2cccc3c(ccc(N1)c23)\N=N\c4ccc(\N=N\c5ccccc5)c6ccccc46 |
| CAS DataBase Reference | 4197-25-5(CAS DataBase Reference) |
| EWG's Food Scores | 1-4 |
| FDA UNII | 9YDL1Q990E |
| EPA Substance Registry System | C.I. Solvent Black 3 (4197-25-5) |
| UNSPSC Code | 12171500 |
| NACRES | NA.47 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS08 |
|---|---|
| Signal word | Warning |
| Hazard statements | H341 |
| Precautionary statements | P201-P202-P280-P308+P313-P405-P501 |
| PPE | Eyeshields, Gloves, type N95 (US) |
| Hazard Codes | F,Xi |
| Risk Statements | 11-36/37/38 |
| Safety Statements | 24/25-16-37/39-26 |
| WGK Germany | 3 |
| RTECS | SD4431500 |
| TSCA | TSCA listed |
| HazardClass | IRRITANT |
| HS Code | 32041900 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Muta. 2 |
| Toxicity | LD50 ivn-mus: 63 mg/kg CSLNX* NX#04918 |
Solvent Black 3 price More Price(47)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | 199664 | Sudan Black B certified by the Biological Stain Commission | 4197-25-5 | 25g | $104 | 2026-04-30 | Buy |
| TCI Chemical | S0113 | Sudan Black B | 4197-25-5 | 25g | $50 | 2026-04-30 | Buy |
| Biosynth | FS33539 | Sudan Black B - Technical | 4197-25-5 | 0.5kg | $421.85 | 2026-06-04 | Buy |
| Biosynth | FS33539 | Sudan Black B - Technical | 4197-25-5 | 1kg | $649 | 2026-06-04 | Buy |
| Biosynth | FS33539 | Sudan Black B - Technical | 4197-25-5 | 2kg | $973.5 | 2026-06-04 | Buy |
Solvent Black 3 Chemical Properties,Uses,Production
Chemical Properties
It is a very dark brown to black fine crystalline powder. It is soluble in ethanol, toluene, acetone, and other solvents. In concentrated sulfuric acid, it is violet-black; when diluted, it is dark greenish-blue and produces a blue to black precipitate. The ethanol solution of the dye is bluish-black when concentrated hydrochloric acid is added and dark blue when concentrated sodium hydroxide solution is added.
Uses
Sudan Black is a basic black dye that is used in staining triglycerides and lipids off some lipoproteins. Biological stain. Dyes and metabolites, Environmental Testing.
Preparation
aniline diazotization, and Naphthalen-1-amine coupling, and its product to diazotization, and 2,2-Dimethyl-2,3-dihydro-1H-perimidinecoupling.
Definition
ChEBI: Sudan black B is a member of the class of perimidines that is 2,2-dimethyl-2,3-dihydro-1H-perimidine carrying a [4-(phenyldiazenyl)naphthalen-1-yl]diazenyl substituent at position 6. A fat-soluble dye predominantly used for demonstrating triglycerides in frozen sections and for staining of protein bound lipids in paraffin sections. It has a role as a histological dye. It is a member of azobenzenes, a bis(azo) compound and a member of perimidines.
Biological Activity
Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn′t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.
Safety Profile
A poison by intravenous route. When heated to decomposition it emits toxic vapors of NOx.
Staining Procedures
1 % Sudan black B is dissolved in 70% ethanol, the tissue is stained 1-1.5 h, subsequently washed in 70% ethanol (3 x 15 s), and mounted in 75% glycerol before microscopy. This staining method reveals positive results for lipids.
Properties and Applications
Blue-black. Soluble in ethanol, acetone and toluene. In concentrated sulfuric acid for purple light black, dilution after deep green light blue, blue to black precipitation. Dye ethanol solution to join concentrated hydrochloric acid for blue black; Join concentrated sodium hydroxide solution for skipper.
| Standard | Light Fastness | Heat-resistant(℃) | water | Sodium Carbonate(5%) | Hydrochloric acid(5%) | |
| Melting point | Stable | |||||
| ISO | General | 126 | 100 | Insoluble | No change | No change |
Solvent Black 3 Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai Biological Technology Development Co., Ltd. | 021-69955236-807 13918189704 | chinaruji@chinaruji.com | China | 5093 | 55 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| TAIYUAN RHF CO.,LTD. | +86 351 7031519 | sales@RHFChem.com | China | 2334 | 56 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Beijing HwrkChemical Technology Co., Ltd | 89508211 18501085097 | sales.bj@hwrkchemical.com | China | 9407 | 55 |
| Pure Chemistry Scientific Inc. | 001-857-928-2050 or 1-888-588-9418 | sales@chemreagents.com | United States | 10174 | 62 |
| Winchem Industrial Co. Ltd | 0574-83851061 | info@win-chemical.com | China | 500 | 55 |
| Shanghai Hanhong Scientific Co.,Ltd. | 021-54306202 13764082696 | info@hanhongsci.com | China | 42917 | 64 |
| Sichuan Kulinan Technology Co., Ltd | 400-1166-196 18981987031 | cdhxsj@163.com | China | 11674 | 57 |
View Lastest Price from Solvent Black 3 manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-11 | SOLVENT BLACK 3
4197-25-5
|
25kg | 0.99 | 20MT | Winchem industrial co limited | ||
 |
2026-09-11 | Solvent Black 3
4197-25-5
|
1kg | 99% | 1000kg | WUHAN FORTUNA CHEMICAL CO., LTD | ||
 |
2026-09-08 | Sudan Black B
4197-25-5
|
100g | BS | 1000 KG | Henan Alfa Chemical Co., Ltd |
-
- SOLVENT BLACK 3
4197-25-5
- $0.00
- 0.99
- Winchem industrial co limited
-
- Solvent Black 3
4197-25-5
- 99%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Sudan Black B
4197-25-5
- BS
- Henan Alfa Chemical Co., Ltd
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