Uses
Immunoprecipitation from RIP lysate:Representative lot data.RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 μg of either a normal rabbit IgG, (Cat. #PP64B), or 5 μg of Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: STAU1) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-STAU1 (Staufen 1) antibody (Cat. # CS204405, 1:1000). Proteins were visualized using One-Step IP-Western kit (GenScript Cat. # L00232) .Arrow indicates STAU1. (Figure 2).Western Blot Analysis: Representative lot data.HepG2 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-STAU1 antibody (Cat. # CS204405, 1:1000 dilution). Proteins were visualized using a Go at Anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.Arrow indicates STAU1 (~63 kDa). (Figure 3).Note: Uncharacterized bands appear at ~22 kDa and ~53 kDa in', 'Research Category
Epigenetics & Nuclear Function', 'Research Sub Category
Transcription Factors
RNA Metabolism & Binding Proteins
