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2′,7′-Dichlorodihydrofluorescein Diacetate

2′,7′-Dichlorodihydrofluorescein Diacetate Structure
2′,7′-Dichlorodihydrofluorescein Diacetate structure
CAS No.
4091-99-0
Chemical Name:
2′,7′-Dichlorodihydrofluorescein Diacetate
Synonyms
DCFH-DA;DCFH;Dichlorofluorescin diacetate;187263;DCFH | DCFH-DA;H2DCFDA / DCFH-DA;H2DCFDA, 10 mM in DMSO;2'',7''-Dichlorofluorescein diac;2',7'-Dichlorofluorescin diacetat;2'，7'-dichlorofluorescein oxalate
CBNumber:
CB8497619
Molecular Formula:
C24H16Cl2O7
Molecular Weight:
487.29
MDL Number:
MFCD00128955
MOL File:
4091-99-0.mol
MSDS File:
SDS
Last updated:2025-09-10 18:36:46
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2′,7′-Dichlorodihydrofluorescein Diacetate Properties

Melting point 209-210 °C(lit.)
Boiling point 593.5±50.0 °C(Predicted)
Density 1.463±0.06 g/cm3(Predicted)
storage temp. -20°C
solubility DMF: soluble
form Crystalline solid
pka 3.79±0.36(Predicted)
color White to off-white
Appearance colorless solid
BRN 4610273
Stability Light Sensitive
InChI InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChIKey PXEZTIWVRVSYOK-UHFFFAOYSA-N
SMILES C(O)(=O)C1=CC=CC=C1C1C2=C(C=C(OC(C)=O)C(Cl)=C2)OC2=C1C=C(Cl)C(OC(C)=O)=C2
EPA Substance Registry System 2',7'-Dichlorohydrofluorescein diacetate (4091-99-0)
UNSPSC Code 12352202
NACRES NA.77

SAFETY

Risk and Safety Statements

Symbol(GHS)  GHS hazard pictograms
GHS07
Signal word  Warning
Hazard statements  H302-H315-H319
Precautionary statements  P501-P270-P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313-P301+P312+P330
Hazard Codes  Xi
Risk Statements  36/37/38
Safety Statements  22-24/25-26
WGK Germany  3
F  10-21
HS Code  29329990
NFPA 704
0
2 0

2′,7′-Dichlorodihydrofluorescein Diacetate price More Price(30)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D6883 2′,7′-Dichlorofluorescin diacetate ≥97% 4091-99-0 50mg $55.1 2025-07-31 Buy
Sigma-Aldrich 287810 2?,7?-Dichlorofluorescin Diacetate 4091-99-0 100mg $110 2025-07-31 Buy
TCI Chemical D4409 2',7'-Dichlorodihydrofluorescein Diacetate 4091-99-0 100MG $137 2025-07-31 Buy
Cayman Chemical 85155 2,7-Dichlorodihydrofluorescein diacetate ≥95% 4091-99-0 50mg $33 2024-03-01 Buy
Cayman Chemical 85155 2,7-Dichlorodihydrofluorescein diacetate ≥95% 4091-99-0 100mg $62 2024-03-01 Buy
Product number Packaging Price Buy
D6883 50mg $55.1 Buy
287810 100mg $110 Buy
D4409 100MG $137 Buy
85155 50mg $33 Buy
85155 100mg $62 Buy

2′,7′-Dichlorodihydrofluorescein Diacetate Chemical Properties,Uses,Production

Description

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 59,500 M−1cm−1).

Chemical Properties

white to yellow-beige or pale pink powder

Uses

Cell-permeable fluorogenic probe used to measure reactive oxygen species (ROS) within cells by detection of enzymatically formed hydrogen peroxide.

Uses

2',7'-Dichlorodihydrofluorescein as a cell-permeable non-fluorescent probe. It is de-esterified intracellularly and turns to highly fluorescent 2’7’dichlorofluorescein upon oxidation.

Uses

2′,7′-Dichlorofluorescin diacetate is a cell-permeable non-fluorescent probe. 2′,7′-Dichlorofluorescin diacetate is de-esterified intracellularly and turns to highly fluorescent 2′,7′-dichlorofluorescein upon oxidation.
Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

General Description

Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (?NO) and for the determination of the degree of overall oxidative stress.

Biochem/physiol Actions

Cell permeable: yes

storage

Store at -20°C

References

[1] N W KOOY  H I  J A Royall. Oxidation of 2’,7’-dichlorofluorescin by peroxynitrite.[J]. Free Radical Research, 1997, 27 3: 245-254. DOI: 10.3109/10715769709065763
[2] TARABOVá B, LUKE? P, HAMMER M U, et al. Fluorescence measurements of peroxynitrite/peroxynitrous acid in cold air plasma treated aqueous solutions[J]. Physical Chemistry Chemical Physics, 2019, 17: 8883-8896. DOI: 10.1039/c9cp00871c
[3] HYEJIN PARK. Oridonin Enhances Radiation-Induced Cell Death by Promoting DNA Damage in Non-Small Cell Lung Cancer Cells.[J]. International Journal of Molecular Sciences, 2018, 19 8. DOI: 10.3390/ijms19082378
[4] THAMIZHINIYAN VENKATESAN  Young K K  Young Woong Choi. Effect of an extraction solvent on the antioxidant quality of Pinus densiflora needle extract[J]. Journal of Pharmaceutical Analysis, 2019, 9 3: Pages 193-200. DOI: 10.1016/j.jpha.2019.03.005
[5] LINA DU. Probenecid and N-Acetylcysteine Prevent Loss of Intracellular Glutathione and Inhibit Neuronal Death after Mechanical Stretch Injury In Vitro.[J]. Journal of neurotrauma, 2016, 33 20 1: 1913-1917. DOI: 10.1089/neu.2015.4342
[6] JIN-YI YANG  Wei Y Y. Spatiotemporally controlled initiation of Parkin-mediated mitophagy within single cells.[J]. Autophagy, 2011: 1230-1238. DOI: 10.4161/auto.7.10.16626

Abs/Em Maxima

511/533 nm

Excitation / Emission Maximum

492 nm/515 nm

Fluoresene quantum yield

0.76

Extinction Coefficient

118626

CF260

0.17

CF280

0.14

2′,7′-Dichlorodihydrofluorescein Diacetate Preparation Products And Raw materials

Raw materials

2',7'-DICHLOROFLUORESCIN DIACETATE

Preparation Products

2',7'-Dichlorofluorescein 2',7'-dichlorodihydrofluorescein

2′,7′-Dichlorodihydrofluorescein Diacetate Suppliers

Global( 283)Suppliers
Supplier Tel Email Country ProdList Advantage
Shaanxi Dideu Medichem Co. Ltd 		+86-29-81139210 +86-18192627656 1059@dideu.com China 3391 58
Hebei Mujin Biotechnology Co.,Ltd 		+8613288715578 sales@hbmojin.com China 12384 58
Henan Tianfu Chemical Co.,Ltd. 		+86-0371-55170693 +86-19937530512 info@tianfuchem.com China 21622 55
ATK CHEMICAL COMPANY LIMITED 		+undefined-21-51877795 ivan@atkchemical.com China 33024 60
CONIER CHEM AND PHARMA LIMITED 		+8618523575427 sales@conier.com China 49732 58
career henan chemical co 		+86-0371-86658258 +8613203830695 factory@coreychem.com China 29801 58
TargetMol Chemicals Inc. 		+1-781-999-5354; +17819995354 marketing@targetmol.com United States 32430 58
Hubei Ipure Biology Co., Ltd 		+8613367258412 ada@ipurechemical.com China 10242 58
Hefei TNJ Chemical Industry Co.,Ltd. 		+86-0551-65418671 +8618949823763 sales@tnjchem.com China 34563 58
HONG KONG IPURE BIOLOGY CO.,LIMITED 		86 18062405514 18062405514 ada@ipurechemical.com CHINA 3461 58
Supplier Advantage
Shaanxi Dideu Medichem Co. Ltd 		58
Hebei Mujin Biotechnology Co.,Ltd 		58
Henan Tianfu Chemical Co.,Ltd. 		55
ATK CHEMICAL COMPANY LIMITED 		60
CONIER CHEM AND PHARMA LIMITED 		58
career henan chemical co 		58
TargetMol Chemicals Inc. 		58
Hubei Ipure Biology Co., Ltd 		58
Hefei TNJ Chemical Industry Co.,Ltd. 		58
HONG KONG IPURE BIOLOGY CO.,LIMITED 		58

View Lastest Price from 2′,7′-Dichlorodihydrofluorescein Diacetate manufacturers

Image Update time Product Price Min. Order Purity Supply Ability Manufacturer
H2DCFDA pictures 2025-09-10 H2DCFDA
4091-99-0
 US $37.00-50.00 / mg 98.68% 10g TargetMol Chemicals Inc.
2′,7′-Dichlorodihydrofluorescein Diacetate pictures 2025-08-08 2′,7′-Dichlorodihydrofluorescein Diacetate
4091-99-0
 US $0.00 / KG 1KG 99% 50000KG/month Hebei Mujin Biotechnology Co.,Ltd
2′,7′-Dichlorodihydrofluorescein Diacetate pictures 2024-08-06 2′,7′-Dichlorodihydrofluorescein Diacetate
4091-99-0
 US $30.00-20.00 / KG 1KG 99% 200000KG Shaanxi Dideu Medichem Co. Ltd
  • H2DCFDA pictures
  • H2DCFDA
    4091-99-0
  • US $37.00-50.00 / mg
  • 98.68%
  • TargetMol Chemicals Inc.

2′,7′-Dichlorodihydrofluorescein Diacetate Spectrum

2′,7′-Dichlorodihydrofluorescein Diacetate(4091-99-0)1HNMR

4091-99-0(2′,7′-Dichlorodihydrofluorescein Diacetate)Related Search:

Adenine Sulfate Dihydrate 2',7'-Dichlorofluorescein 2′,7′-Dichlorodihydrofluorescein Diacetate 2',7'-DICHLOROFLUORESCIN DIACETATE 5-(AND-6)-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE
5-(and-6)-carboxy-2`,7`-dichlorofluorescein diacetate 6-Carboxy-2',7'-dichlorofluorescein 3',6'-Diacetate SucciniMidyl Ester 5(6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER 2'',7''-DICHLOROFLUORESCIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE (2',7'-DICHLOROFLUORESCIN DIACETATE; H2DCFDA)
5-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER 6-CARBOXY-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, DI(ACETOXYMETHYL ESTER) 6-CARBOXY-2'',7''-DICHLORODIHYDROFLUORESCIN DIACETATE, DI(ACETOXYMETHYL ESTER) CALCIUM GREEN(TM)-2, AM
5-(AND-6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN DIACETATE 5-(AND-6)-CHLOROMETHYL-2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE 2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE, SUCCINIMIDYL ESTER

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187263 2,7-Dichlorofluorescin Diacetate - CAS 4091-99-0 - Calbiochem 2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate 2',7'-DICHLOROFLUORESCIN DIACETATE, FOR FLUORESCENCE 2',7'-Dichlorodihydrofluoresceinediacetate Dichlorofluorescin diacetate 2'',7''-Dichlorofluorescein diac 2-(2,7-Dichloro-3,6-diacetoxy-9H-xanthen-9-yl)benzoic acid 2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid 2-(3,6-diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid 2',7'-DICHLOROFLUORESCIN DI-ACETATE ANALYTICAL GRADE 2′,7′-Dichlorofluorescin diacetate,2′,7′-Dichlorodihydrofluorescein diacetate 2',7'-Dichlorofluorescin diacetat DCFH o-(2,7-Dichloro-3,6-dihydroxyxanthen-9-yl)benzoic Acid Diacetate Dichlorofluorescin diacetate analytical grade H2DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] DCFH-DA H2DCFDA [2',7'-DICHLORODIHYDROFLUORESCEIN DIACETATE];DCFH-DA 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl) -Dichlorodihydrofluorescein diacetate 2',7'-Dichlorodihydrofluorescein diacetate [2',7'-Dichlorofluorescin diacetate] *CAS 4091-99-0* 2' 7'-DICHLOROFLUORESCIN DIACETATE FOR& 2-[3,6-bis(acetyloxy)-2,7-dichloro-9h-xanthen-9-yl]-benzoicaci Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]- H2DCFDA[2',7'-DICHLORODIHYDROFLUORESCEINDIACETATChemicalbookE] DCFH | DCFH-DA H2DCFDA / DCFH-DA 2′,7′-Dichlorofluorescin diacetate, ≥97% 2,7-Dichlorofluorescein Diacetate (DCFH) extrapure, 97% 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoicaci 2',7'-Dichlorodihydrofluorescein diacetate 2',7'-Dichlorodihydrofluorescein diacetate|||DCFH-DA 2'，7'-dichlorofluorescein oxalate 2',7'-Dichlorofluorescein diacetate DCFH-DA reactive oxygen species ROS fluorescent probe 2'', 7''-Dichlorofluorescin diacetate H2DCFDA, 10 mM in DMSO H2-DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] - 100 mg H2-DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] - 100 mg 2'，7'-dichlorofluorescein oxalate DCFH-DA ROS Fluorescent Probes (Green) 4091-99-0 C24H16Cl2O7