Acetylcholine chloride
- CAS No.
- 60-31-1
- Chemical Name:
- Acetylcholine chloride
- Synonyms
- ACH;ovisot;tl1505;miochol;acecholin;ACECOLINE;achchloride;arterocoline;ARTEROCHOLINE;Acetylcholine Cl
- CBNumber:
- CB9216208
- Molecular Formula:
- C7H16ClNO2
- Molecular Weight:
- 181.66
- MDL Number:
- MFCD00011698
- MOL File:
- 60-31-1.mol
- MSDS File:
- SDS
- TDS File:
- TDS
| Melting point | 146-150 °C (lit.) |
|---|---|
| Density | 1.1235 (rough estimate) |
| refractive index | 1.5560 (estimate) |
| storage temp. | room temp |
| solubility | H2O: 0.1 g/mL, clear, colorless |
| form | Crystalline Powder |
| color | White |
| biological source | synthetic |
| Water Solubility | Soluble IN COLD WATER |
| Sensitive | Hygroscopic |
| Merck | 14,87 |
| BRN | 3571875 |
| Stability | Stable. Substances to be avoided include strong oxidizing agents. Protect from moisture - very hygroscopic. |
| InChI | 1S/C7H16NO2.ClH/c1-7(9)10-6-5-8(2,3)4;/h5-6H2,1-4H3;1H/q+1;/p-1 |
| InChIKey | JUGOREOARAHOCO-UHFFFAOYSA-M |
| SMILES | [Cl-].CC(=O)OCC[N+](C)(C)C |
| LogP | -3.904 (est) |
| CAS DataBase Reference | 60-31-1(CAS DataBase Reference) |
| FDA UNII | AF73293C2R |
| EPA Substance Registry System | Ethanaminium, 2-(acetyloxy)-N,N,N-trimethyl-, chloride (60-31-1) |
| UNSPSC Code | 51151535 |
| NACRES | NA.75 |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319-H303-H335 | |||||||||
| Precautionary statements | P264-P280-P302+P352+P332+P313+P362+P364-P305+P351+P338+P337+P313-P261-P280a-P304+P340-P305+P351+P338-P405-P501a | |||||||||
| PPE | dust mask type N95 (US), Eyeshields, Gloves | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 26-37/39-36 | |||||||||
| WGK Germany | 2 | |||||||||
| RTECS | FZ9800000 | |||||||||
| F | 3-8-10-21 | |||||||||
| TSCA | TSCA listed | |||||||||
| HazardClass | IRRITANT | |||||||||
| HS Code | 29379000 | |||||||||
| Storage Class | 11 - Combustible Solids | |||||||||
| Toxicity | LD50 in mice, rats (mg/kg): 20, 22 i.v., 170, 250 s.c., 3000, 2500 i.p. (Molitor) | |||||||||
| NFPA 704 |
|
Acetylcholine chloride price More Price(88)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich | Y0000002 | Acetylcholine chloride European Pharmacopoeia (EP) Reference Standard | 60-31-1 | 50 mg | $167 | 2026-04-30 | Buy |
| Sigma-Aldrich | RDD043 | Acetylcholine chloride ≥99% (TLC), free-flowing, Redi-Dri? | 60-31-1 | 100G | $284 | 2026-04-30 | Buy |
| Sigma-Aldrich | A9101 | Acetylcholine chloride pkg of 150?mg (per vial) | 60-31-1 | 10vials | $358 | 2026-04-30 | Buy |
| Sigma-Aldrich | A2661 | Acetylcholine chloride suitable for cell culture | 60-31-1 | 25g | $89.2 | 2026-04-30 | Buy |
| Sigma-Aldrich | 1008501 | Acetylcholine chloride United States Pharmacopeia (USP) Reference Standard | 60-31-1 | 200mg | $455 | 2026-04-30 | Buy |
Acetylcholine chloride Chemical Properties, Uses, Production
Description
Acetylcholine is a neurotransmitter that binds to nicotinic and muscarinic acetylcholine receptors (AChRs) in the central and peripheral nervous systems. It mediates motor function at the neuromuscular junction but also has functions in the parasympathetic and sympathetic nervous systems. It is involved in learning and memory through actions at nicotinic AChRs in the CNS. The actions of acetylcholine are terminated primarily via the action of acetylcholinesterase, which breaks it down into acetate and choline. Acetylcholine (chloride) mimics the effects of acetylcholine and has been used to determine the function of acetylcholine in various biological processes. Acetylcholine (chloride) inhibits peptide aggregation of p53 mutants in vitro at micromolar concentrations. It increases alveolar fluid clearance in a dose-dependent manner and enhances Na+/K+-ATPase activity, effects which are blocked by atropine , in a mouse model of pulmonary edema.
Chemical Properties
A white or almost white crystalline powder, or colorless crystals, highly hygroscopic, with a faint ammoniacal odor and a very salty taste. Melting point: 149-152°C. The pH of a 10% aqueous solution is approximately 5, and the substance rapidly decomposes in hot water and alkaline solutions. It is highly soluble in water, ethanol, and propylene glycol; soluble in chloroform and acetic acid; and insoluble in ether. The product exhibits low toxicity, with an LD50 (oral, rat) of 2500 mg/kg.
Uses
Acetylcholine Chloride is a cholinergic, antiarrhythmic, miotic, vasodilator (peripheral).
Definition
ChEBI: Acetylcholine chloride is the chloride salt of acetylcholine, and a parasympatomimetic drug. It contains an acetylcholine.
Application
Acetylcholine Chloride is a cholinergic neurotransmitter that can induce the opening of calcium channels and endogenous neurotransmitter at cholinergic synapses; amplifies sarcolemma action potential inducing muscle contractions.
Preparation
Acetylcholine chloride is synthesized by reacting chloroethanol and trimethylamine aqueous solution, followed by acylation with acetic anhydride.
brand name
Miochol (Novartis).
General Description
Acetylcholine chloride exerts a powerfulstimulant effect on the parasympathetic nervous system.Attempts have been made to use it as a cholinergic agent,but its duration of action is too short for sustained effects,because of rapid hydrolysis by esterases and lack of specificitywhen administered for systemic effects. It is a cardiacdepressant and an effective vasodilator. Stimulation of thevagus and the parasympathetic nervous system produces atonic action on smooth muscle and induces a flow from thesalivary and lacrimal glands. Its cardiac-depressant effectresults from (a) a negative chronotropic effect that causes adecrease in heart rate and (b) a negative inotropic action onheart muscle that produces a decrease in the force of myocardialcontractions.
Biological Activity
Endogenous neurotransmitter. Acts at nicotinic and muscarinic acetylcholine receptors.
Biochem/physiol Actions
Acetylcholine chloride, injected at 20 mg/kg body weight, reduces mortality and plasma proinflammatory cytokines in mice with experimentally-induced sepsis . The cholinergic anti-inflammatory mechanism is probably mediated by interaction of acetylcholine with α7n cholinoreceptor on monocytes, macrophages, and neutrophils, which decreases the levels of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6.
Clinical Use
Atropine blocks the depressant effect of ACh on cardiacmuscle and its production of peripheral vasodilation (i.e.,muscarinic effects) but does not affect the skeletal musclecontraction (i.e., nicotinic effect) produced.ACh chloride is a hygroscopic powder that is available inan admixture with mannitol to be dissolved in sterile waterfor injection shortly before use. It is a short-acting mioticwhen introduced into the anterior chamber of the eye and isespecially useful after cataract surgery during the placementof sutures. When applied topically to the eye, it has littletherapeutic value because of poor corneal penetration andrapid hydrolysis by AChE.
Safety Profile
Poison by subcutaneous, intravenous, and parenteral routes. Moderately toxic by ingestion. When heated to decomposition it emits very toxic fumes of NOx, and Cl-. A cholinergic agent. See also CHOLINE ACETATE (ESTER).
storage
Desiccate at RT
Purification Methods
It is very soluble in H2O (>10%), and is very hygroscopic. If pasty, dry it in a vacuum desiccator over H2SO4 until a solid residue is obtained. Dissolve this in absolute EtOH, filter it and add dry Et2O, when the hydrochloride separates. Collect by filtration and store it under very dry conditions. [Jones & Major J Am Chem Soc 52 307 1930.] The chloroplatinate crystallises from hot H2O in yellow needles and can be recrystallised from 50% EtOH, m 242-244o [Dudley Biochem J 23 1069 1929]; other m given is 256-257o. The perchlorate crystallises from EtOH as prisms m 116-117o. [J Am Pharm Assocn 36 272 1947, Beilstein 4 IV 1446.]
References
[1] PRASHANT TIWARI . Basic and modern concepts on cholinergic receptor: A review[J]. Asian Pacific Journal of Tropical Disease, 2013, 3 5: Pages 413-420. DOI: 10.1016/s2222-1808(13)60094-8
[2] ZHAOLIN CHEN M K. Inhibition of p53 Mutant Peptide Aggregation In Vitro by Cationic Osmolyte Acetylcholine Chloride.[J]. Protein and Peptide Letters, 2017, 57 1: 353-357. DOI: 10.2174/0929866524666170123142858
[3] XIA LI . Endogenous acetylcholine increases alveolar epithelial fluid transport via activation of alveolar epithelial Na,K-ATPase in mice[J]. Respiratory Physiology & Neurobiology, 2015, 217: Pages 25-31. DOI: 10.1016/j.resp.2015.05.005
Acetylcholine chloride Preparation Products And Raw materials
| Supplier | Tel | Country | ProdList | Advantage | |
|---|---|---|---|---|---|
| Shanghai Xingui Biotechnology Co., Ltd. | 15121041756 | xingui2022@126.com | China | 9954 | 58 |
| Hubei Hicks Biochemical Co., Ltd | 17362916295; 17362916295 | w17362916295@163.com | China | 3985 | 58 |
| J & K SCIENTIFIC LTD. | 18210857532 18210857532 | jkinfo@jkchemical.com | China | 96815 | 76 |
| Meryer (Shanghai) Chemical Technology Co., Ltd. | 4006356688 18621169109 | market03@meryer.com | China | 40202 | 62 |
| 3B Pharmachem (Wuhan) International Co.,Ltd. | 18930552037 | 3bsc@sina.com | China | 15813 | 69 |
| Alfa Aesar | 400-6106006 | saleschina@alfa-asia.com | China | 30103 | 84 |
| TCI (Shanghai) Development Co., Ltd. | 021-67121386 | Sales-CN@TCIchemicals.com | China | 24512 | 81 |
| Energy Chemical | 400-0056266 | sales8178@energy-chemical.com | China | 44850 | 61 |
| Adamas Reagent, Ltd. | 400-6009262 15618770481 | yhx@titansci.com | China | 14098 | 59 |
| Nanjing Chemlin Chemical Co., Ltd | 025-83697070 13770331960 | info@chemlin.com.cn | China | 16305 | 64 |
View Latest Price from Acetylcholine chloride manufacturers
| Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
|---|---|---|---|---|---|---|---|---|
 |
2026-09-17 | Acetylcholine chloride
60-31-1
|
0.99 | RongNa Biotechnology Co.,Ltd | ||||
 |
2026-09-14 | Acetylcholine chloride
60-31-1
|
$100.00 | 1KG | 99% | 1000 KG | HANGZHOU DINGYAN CHEM CO.,LTD | |
 |
2026-08-20 | Acetylcholine chloride USP/EP/BP
60-31-1
|
$1.10 | 1g | 99.9% | 100 Tons min | Dideu Industries Group Limited |
-
- Acetylcholine chloride
60-31-1
- 0.99
- RongNa Biotechnology Co.,Ltd
-
- Acetylcholine chloride
60-31-1
- $100.00
- 99%
- HANGZHOU DINGYAN CHEM CO.,LTD
-
- Acetylcholine chloride USP/EP/BP
60-31-1
- $1.10
- 99.9%
- Dideu Industries Group Limited
