STF-1 (D1Z2A) XP Rabbit mAb

Source

Rabbit

Reactivity

Human;Mouse

Background

The orphan nuclear receptor, steroidogenic factor 1, is encoded by the NR5A1 gene and plays an instrumental role in directing the transcriptional control of steroidogenesis. Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, research studies of both global and tissue-specific knockout mice have demonstrated that STF-1 is required for the development of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with mutations that render STF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency. Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis and adrenocortical carcinoma.Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an amino-terminal zinc finger DNA-binding domain, a ligand-binding domain, a carboxy-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box, which functions as an accessory DNA binding domain. STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors. In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function, acetylation enhances its transcriptional activity.

References

[1] Parker, K.L. and Schimmer, B.P. (1997) Endocr Rev 18, 361-77.
[2] Luo, X. et al. (1994) Cell 77, 481-90.
[3] Zhao, L. et al. (2001) Development 128, 147-54.
[4] Jeyasuria, P. et al. (2004) Mol Endocrinol 18, 1610-9.
[5] Pelusi, C. et al. (2008) Biol Reprod 79, 1074-83.
[6] Zhao, L. et al. (2008) Mol Endocrinol 22, 1403-15.
[7] Achermann, J.C. et al. (1999) Nat Genet 22, 125-6.
[8] Lourenço, D. et al. (2009) N Engl J Med 360, 1200-10.
[9] Bulun, S.E. et al. (2009) Mol Cell Endocrinol 300, 104-8.
[10] Figueiredo, B.C. et al. (2005) J Clin Endocrinol Metab 90, 615-9.

Raw materials

Preparation Products