EGFR Extracellular Domain (human, recombinant)

Description

Epidermal growth factor receptor (EGFR), also known as HER1 and ERBB1, is a cell surface receptor and member of the EGF family of receptor tyrosine kinases with roles in cell proliferation, differentiation, and survival.1,2 It is a 170 kDa transmembrane receptor composed of an intracellular tyrosine kinase domain, a transmembrane lipophilic segment, and an extracellular domain that is expressed in epithelial, mesenchymal, and neuronal tissues.1,2,3 Under unstimulated conditions, EGFR is an auto-inhibited monomer in the plasma membrane.1 Upon canonical ligand binding, EGFR undergoes homodimerization or heterodimerization with HER2, HER3, or HER4, which induces a conformational change in the cytoplasmic domain that facilitates autophosphorylation and intracellular signaling. EGFR inhibits autophagy under nutrient-rich growth conditions and, conversely, induces autophagy under serum-starved conditions by interacting with the autophagy inhibitor Rubicon to induce its dissociation from Beclin-1. Overexpression of EGFR is found in multiple solid tumors, including renal, breast, ovarian, and head and neck cancer, as well as non-small cell lung cancer (NSCLC).2 EGFRL858R is associated with increased susceptibility to tyrosine kinase inhibition and cell death, while EGFRT790M is associated with kinase inhibitor resistance in NSCLC.4 Inhibition of EGFR reduces angiotensin II-induced cardiac hypertrophy in mice.5 Cayman’s EGFR Extracellular Domain (human, recombinant) protein can be used for ELISA. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of the EGFR extracellular domain (amino acids 25-645) fused to human IgG1 Fc at its C-terminus, consists of 860 amino acids and has a calculated molecular weight of 95 kDa, and a predicted N-terminus of Leu25 after signal peptide cleavage. As a result of glycosylation, the monomer migrates at approximately 130 to 140 kDa by SDS-PAGE under reducing conditions.WARNING This product is not for human or veterinary use.