2-ニトロフェニルβ-D-ガラクトピラノシド
2-ニトロフェニルβ-D-ガラクトピラノシド 物理性質
- 融点 :
- 186-193 °C (dec.)
- 比旋光度 :
- -70 º (c=1, H2O)
- 沸点 :
- 442.39°C (rough estimate)
- 比重(密度) :
- 1.3709 (rough estimate)
- 屈折率 :
- -68.8 ° (C=1, H2O)
- 貯蔵温度 :
- 2-8°C
- 溶解性:
- DMSO（微量）、水（微量、加熱、超音波処理）
- 酸解離定数(Pka):
- 12.69±0.70(Predicted)
- 外見 :
- 粉
- 色:
- 白色～わずかに黄色
- 旋光度（Optical Rotation）:
- [α]20/D 70±1°, c = 1% in H2O
- 水溶解度 :
- 水に溶ける。
- BRN :
- 92207
- InChI:
- 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
- InChIKey:
- KUWPCJHYPSUOFW-RMPHRYRLSA-N
- SMILES:
- OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
- CAS データベース:
- 369-07-3(CAS DataBase Reference)
- NISTの化学物質情報:
- Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)
2-ニトロフェニルβ-D-ガラクトピラノシド 価格
もっと(28)
| メーカー |
製品番号 |
製品説明 |
CAS番号 |
包装 |
価格 |
更新時間 |
購入 |
|
富士フイルム和光純薬株式会社(wako)
|
W01MPB02102473 |
o-ニトロフェニル-β-D-ガラクトピラノシド, 結晶
o-Nitrophenyl-beta-D-galactopyranoside, Crystalline |
369-07-3 |
500mg |
￥6800 |
2024-03-01 |
購入 |
|
富士フイルム和光純薬株式会社(wako)
|
W01MPB02102473 |
o-ニトロフェニル-β-D-ガラクトピラノシド, 結晶
o-Nitrophenyl-beta-D-galactopyranoside, Crystalline |
369-07-3 |
1g |
￥7500 |
2024-03-01 |
購入 |
|
富士フイルム和光純薬株式会社(wako)
|
W01MPB02102473 |
o-ニトロフェニル-β-D-ガラクトピラノシド, 結晶
o-Nitrophenyl-beta-D-galactopyranoside, Crystalline |
369-07-3 |
5g |
￥10900 |
2024-03-01 |
購入 |
|
富士フイルム和光純薬株式会社(wako)
|
W01MPB02102473 |
o-ニトロフェニル-β-D-ガラクトピラノシド, 結晶
o-Nitrophenyl-beta-D-galactopyranoside, Crystalline |
369-07-3 |
25g |
￥42400 |
2024-03-01 |
購入 |
|
東京化成工業
|
N0418 |
2-ニトロフェニルβ-D-ガラクトピラノシド >98.0%(HPLC)
2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]
>98.0%(HPLC) |
369-07-3 |
1g |
￥3500 |
2024-03-01 |
購入 |
2-ニトロフェニルβ-D-ガラクトピラノシド 化学特性,用途語,生産方法
外観
白色～わずかにうすい褐色, 結晶性粉末～粉末
溶解性
水, メタノールにやや溶けにくく、エタノールに溶けにくく、ジエチルエーテルにほとんど溶けない。
用途
β-D-galactosidaseの測定用基質。
説明
2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
化学的特性
White Crystalline Solid
使用
2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
定義
ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
一般的な説明
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
純化方法
Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a
chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]
2-ニトロフェニルβ-D-ガラクトピラノシド 上流と下流の製品情報
原材料
準備製品
2-ニトロフェニルβ-D-ガラクトピラノシド 生産企業
Global( 543)Suppliers
2-ニトロフェニルβ-D-ガラクトピラノシド スペクトルデータ(1HNMR、IR1、IR2、MS、Raman)
369-07-3(2-ニトロフェニルβ-D-ガラクトピラノシド)キーワード:
- 369-07-3
- 2-Nitrophenyl hexopyranoside
- 2-Nitrophenyl-beta-delta-galactopyranoside
- 2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
- o-Nitrophenyl-β-D-galactopyranoside - CAS 369-07-3 - Calbiochem
- beta-D-Galactopyranoside, 2-nitrophenyl
- beta-d-galactopyranoside,2-nitrophenyl
- Galactopyranoside, o-nitrophenyl, beta-D-
- o-Nitrophenyl beta-galactoside
- ONPG o-Nitrophenyl-?-D-galactoside
- 2'-Nitrophenyl-D-galactopyranoside (ONPG)
- 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE, 99+%
- 2-Nitrophenyl-B-D-Galactopyranoside(Onpg)
- 2-Nitrophenyl-Beta-D-Galactopyranside
- O-Nitrophenyl-Beta-D-GalactopyranosideA.R.
- o-NPG=ONPG
- Nitrophenyl-b-D-galactopyranoside
- 2-nitrophenyl-á-d-galactopyranoside
- ONPG（2-Nitrophenyl-beta-D-galactopyranoside
- 2-Nitrophenyl b-D-galactoside-(1,5), ONPG
- 2-Nitrophenyl-β-D-galactoside
- 2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]
- ortho-Nitrophenyl-β-galactoside
- (2R,3R,4S,5R,6S)-2-(hydroxyMethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
- Ortho nitroalpha -D-glucopyranoside
- The 2- nitrophenyl beta-D- galactopyranoside
- ortho-nitrophenyl β-D-galactopyranoside
- 2-nitrophenyl β-d-[1-13C]galacto-pyranoside
- 2-nitrophenyl β-d-[2-13C]galacto-pyranoside
- TOLUIDINE BLUE O HIGH PURITY GRADE
- 2-Nitrophenyl--D-galactopyranoside for synthesis
- 2-ニトロフェニルβ-D-ガラクトピラノシド
- 2-ニトロフェニルβ-D-galacto-ヘキソピラノシド
- o-ニトロフェニルβ-D-ガラクトピラノシド
- o-ニトロフェニル-β-D-ガラクトピラノシド
- (2-ニトロフェニル)β-D-ガラクトピラノシド
- 2-ニトロフェニル-Β-D-ガラクトピラノシド
- 2‐ニトロフェニル‐Β‐D‐ガラクトピラノシド
- 2-ニトロフェニル Β-D-ガラクトピラノシド
- O-ニトロフェニル-Β-D-ガラクトピラノシド, 結晶
- NITROPHENYL-B-D-GALACTOPYRANOSIDE RESEARCH GRADE
- ニトロフェニル-Β-D-ガラクトピラノシド, 2-
- 1-O-(2-ニトロフェニル)-β-D-ガラクトピラノース
- (2R,3R,4S,5R,6S)-2-(ヒドロキシメチル)-6-(2-ニトロフェノキシ)オキサン-3,4,5-トリオール
- ガラクトース
- グリコシド
- 生化学
- 糖