Biotin-16-UTP 化学特性,用途語,生産方法
説明
Biotin-16-UTP is a biotin-labeled UTP used for in vitro transcription to prepare RNA probes[1]. Biotin-16-UTP is an active substrate for RNA polymerase and can be incorporated by a variety of RNA polymerases (including SP6, T3, and T7 RNA polymerases) to form labeled RNA[2, 3]. Biotin-16-UTP-labeled RNA can be detected by reporter molecules linked to streptavidin, avidin, or anti-biotin antibodies[4, 5]. Biotin-16-UTP can be used for molecular hybridization and RNA-protein interaction studies[6].
使用
Biotin-labeled RNA can be used as a hybridization probe for:
- Northern blots
- Southern blots
- Plaque or colony lifts
- RNase protection experiments
- In situ hybridization
- Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.
一般的な説明
For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the
in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is
in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.
Biotin-16-UTP 上流と下流の製品情報
原材料
準備製品