フルオ 4-AMスペシャルパッケージ+DMSO (1ML)
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- ￥37000 - ￥50800
- 化学名: フルオ 4-AMスペシャルパッケージ+DMSO (1ML)
- 英語名: FLUO-4-AM FOR FLUORESCENCE PGE. WITH 5
- 別名:フルオ 4-AMスペシャルパッケージ+DMSO (1ML);フルオ4-AM;2,2′-[[2-[2-[2-[ビス[2-(アセトキシメトキシ)-2-オキソエチル]アミノ]-5-メチルフェノキシ]エトキシ]-4-[2,7-ジフルオロ-6-(アセトキシメトキシ)-3-オキソ-3H-キサンテン-9-イル]フェニル]イミノ]ビス(酢酸アセトキシメチル);(アセチルオキシ)メチル 2-({2-[2-(5-{6-[(アセチルオキシ)メトキシ]-2,7-ジフルオロ-3-オキソ-3H-キサンテン-9-イル}-2-[ビス({2-[(アセチルオキシ)メトキシ]-2-オキソエチル})アミノ]フェノキシ)エトキシ]-4-メチルフェニル}({2-[(アセチルオキシ)メトキシ]-2-オキソエチル})アミノ)アセタート;N-[2-(アセトキシメトキシ)-2-オキソエチル]-N-[2-[2-[5-[2,7-ジフルオロ-3-オキソ-6-(アセトキシメトキシ)-3H-キサンテン-9-イル]-2-[ビス[2-(アセトキシメトキシ)-2-オキソエチル]アミノ]フェノキシ]エトキシ]-4-メチルフェニル]グリシンアセトキシメチル
- CAS番号: 273221-67-3
- 分子式: C51H50F2N2O23
- 分子量: 1096.94
- EINECS:
- MDL Number:MFCD18837018
- 富士フイルム和光純薬株式会社(wako)
- 1mg
- 50μg×8
- 生産者富士フイルム和光純薬株式会社(wako)
- 製品番号W01T02F312
- 製品説明フルオ 4-AMスペシャルパッケージ+DMSO (1ml)
- 英語製品説明Fluo 4-AM Special Packaging + DMSO (1ml)
- 包装単位50μg×8
- 価格￥37000
- 更新しました2024-03-01
- 購入
- 生産者富士フイルム和光純薬株式会社(wako)
- 製品番号W01T02F311
- 製品説明フルオ4-AM
- 英語製品説明Fluo 4-AM
- 包装単位1mg
- 価格￥50800
- 更新しました2024-03-01
- 購入
| 生産者 | 製品番号 | 製品説明 | 包装単位 | 価格 | 更新時間 | 購入 |
|---|---|---|---|---|---|---|
| 富士フイルム和光純薬株式会社(wako) | W01T02F312 | フルオ 4-AMスペシャルパッケージ+DMSO (1ml) Fluo 4-AM Special Packaging + DMSO (1ml) |
50μg×8 | ￥37000 | 2024-03-01 | 購入 |
| 富士フイルム和光純薬株式会社(wako) | W01T02F311 | フルオ4-AM Fluo 4-AM |
1mg | ￥50800 | 2024-03-01 | 購入 |
プロパティ
沸点 :1063.2±65.0 °C(Predicted)
比重(密度) :1.47
貯蔵温度 :−20°C
溶解性 :DMSO: soluble
外見 :Solid
酸解離定数(Pka) :1.92±0.50(Predicted)
色 :Brown to reddish brown
外観 :orange-red powder
極大吸収波長 (λmax) :456nm
生物学的応用 :Calcium indicator
InChIKey :QOMNQGZXFYNBNG-UHFFFAOYSA-N
SMILES :C(OCOC(C)=O)(=O)CN(C1=CC=C(C2C3=C(C=C(OCOC(C)=O)C(F)=C3)OC3C=2C=C(F)C(=O)C=3)C=C1OCCOC1=CC(C)=CC=C1N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
安全情報
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説明
Fluo-4 AM is a cell-permeable Ca2+-indicator that is metabolized by intracellular esterase, leading to a bright green fluorescent signal upon Ca2+-binding (excitation/emission λ at 494/506 nm). Fluo-4 AM is used for visualization and measurement of intracellular Ca2+. It is well suited for fluorometric and imaging applications such as microscopy, flow cytometry, spectrofluorometry, and fluorometric high-throughput microplate screening assays [1].
Fluo-4 AM is similar in structure and spectral properties to the widely used Ca2+-indicator, Fluo-3, but it has certain advantages over Fluo-3, such as brighter fluorescence emission, high rate of cell permeation, and a Kd for Ca2+ in buffer of 345 nM. Because of its higher fluorescence emission intensity, Fluo-4 AM can be used at lower intracellular concentrations, making its use less toxic for live cells.
As Fluo-4 AM does not covalently bind to cellular components, it may be actively effluxed from the cell by organic anion transporters. In vivo cell imaging with Fluo-4 AM is usually performed within one or two hours after loading, but the dye can be re-loaded to cells if it is needed. Fluo-4 AM can also be fixed in situ by EDC/EDAC for downstream immunofluorescence studies.
Fluo-4 AM has low solubility in the water. It is recommended to prepare 1 mM stock solution in labeling grade DMSO prior to cell loading. Use the final concentration of 1-5 μM and incubation at 37 °C for 15-60 min as a start point of your protocol.