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| | Alexa Fluor 350 Basic information |
| Product Name: | Alexa Fluor 350 | | Synonyms: | Alexa Fluor 350;BF 350, SE;2H-1-Benzopyran-3-acetic acid, 7-amino-4-methyl-2-oxo-6-sulfo-, 3-(2,5-dioxo-1-pyrrolidinyl) ester;Alexa Fluor 350 NHS ester;AF350 NHS Ester;Aladdin? 350 NHS Ester(Succinimidyl Ester);BP Fluor 350 NHS Ester;Adenosine 5′-(tetrahydrogen triphosphate), 3′-deoxy-, trisodium salt | | CAS: | 200554-19-4 | | MF: | C16H14N2O9S | | MW: | 410.35 | | EINECS: | | | Product Categories: | | | Mol File: | 200554-19-4.mol |  |
| | Alexa Fluor 350 Chemical Properties |
| density | 1.74±0.1 g/cm3(Predicted) | | solubility | Soluble in water, dimethyl sulfoxide, methanol | | pka | 0.46±0.20 Most Basic Temperature: 25°C | | color | Pale yellow precipitate2 or yellow/ orange solid | | Appearance | Gray yellow solid | | ex/em | 346/445 nm | | ε(extinction coefficient) | 19,000 | | Φ(quantum yield) | 0.73 | | λmax | 346nm (Buffer pH 7); 353nm (MeOH) | | Major Application | Poly(vinyl alcohol)coated microfluidic devices; selfassembled monolayers; silica particles/beads | | Biological Applications | Analyzing peptides; detecting mutations; detecting/identifying nucleic acids; detecting/isolating/purifying proteins; investigating location of phosphatidylinositol,bisphosphate (PI(,)P) in cell membranes; colloidal diagnostic devices; multiplex genetic analysis; as nucleic acid hybridization probe; useful for synthesizing nucleic acid,, peptides, |
| | Alexa Fluor 350 Usage And Synthesis |
| Chemical Properties | Appearance: Grayish yellow solid
ex/em: 346/445 nm
Extinction coefficient (ε): 19,000
Quantum yield (Φ): 0.73 | | Uses | BP Fluor 350 NHS ester is an amine reactive, water soluble, blue emitting dye used to specifically and efficiently modify primary amines at pH 7-9 to form stable covalent amide bonds[1]. | | Definition | ChEBI: Alexa Fluor 350 is a chromenone, a pyrrolidinone and an organosulfonic acid. It has a role as a fluorochrome. | | References | [1] Khoury JT, et al. Pulsed fluorescence measurements of trapped molecular ions with zero background detection. J Am Soc Mass Spectrom. 2002 Jun;13(6):696-708. DOI:10.1016/S1044-0305(02)00364-1 |
| | Alexa Fluor 350 Preparation Products And Raw materials |
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