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| | FADD Antibody Chemical Properties |
| | FADD Antibody Usage And Synthesis |
| Source | Rabbit | | Applications | FADD antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Recommended dilution range for Western blot is 1:1,000~1:2,000 and immunohistochemistry analysis is 1:50~100. Recommended startin | | Reactivity | Human | | Immunogen | Anti-human FADD mAb is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human FADD amino acids 1-208 purified from E. coli. | | Background | FADD is an adaptor protein that cooperates with a variety of cell surface receptors and mediates cell apoptotic signals. Using its C-terminal death domain, FADD is recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and consequently it take parts in the death signaling initiated by these receptors. FADD interaction with the receptors reviels the N-terminal effector domain of, which allows it to recruit caspase-8, and thus initiate the cysteine protease cascade. Knockout studies in mice furthermore propose the significance of FADD in premature T cell development. FADD plays a role in survival/proliferation and cell cycle development. FADD also takes part in cellular sublocalization, protein phosphorylation, and inhibitory molecules. | | References | [1] Ashkenazi, A. and Dixit, V.M. (1998) Science 281, 1305-1308.
[2] Kuang, A. A. et al. (2000) J. Biol. Chem. 275, 25065-25068.
[3] Scaffidi, C. et al. (2000) J. Immunol. 164, 1236-1242.
[4] Newton, K. et al. (2000) EMBO J. 19, 931-941.
[5] Zhang, J. et al. (2001) J. Biol. Chem. 276, 29815-29818. |
| | FADD Antibody Preparation Products And Raw materials |
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