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| | NAPHTHOL AS-BI PHOSPHATE Basic information |
| Product Name: | NAPHTHOL AS-BI PHOSPHATE | | Synonyms: | 2-NAPHTHALENECARBOXAMIDE, 7-BROMO-N-(2-METHOXYPHENYL)-3-(PHOSPHONOOXY);7-BROMO-N-(2-METHOXYPHENYL)-3-(PHOSPHONOOXY)-2-NAPHTHALENECARBOXAMIDE;7-BROMO-3-HYDROXY-2-NAPHTHOIC-O-ANISIDE PHOSPHATE;7-BROMO-3-HYDROXY-2-NAPHTHOIC-O-ANISIDIDE PHOSPHATE;6-BROMO-2-PHOSPHOHYDROXY-3-NAPHTHOIC ACID O-ANISIDIDE;NAPHTHOL AS-BL PHOSPHORIC ACID;NAPHTHOL AS-BI PHOSPHATE;NAPHTHOL AS-BI PHOSPHORIC ACID | | CAS: | 1919-91-1 | | MF: | C18H15BrNO6P | | MW: | 452.19 | | EINECS: | 217-645-0 | | Product Categories: | Substrates;Cytochemistry Products;Hematology and Histology | | Mol File: | 1919-91-1.mol |  |
| | NAPHTHOL AS-BI PHOSPHATE Chemical Properties |
| density | 1.679±0.06 g/cm3(Predicted) | | Fp | 37 °C | | storage temp. | 2-8°C | | solubility | methanol: soluble50mg/mL | | pka | 0.73±0.30(Predicted) | | form | Crystalline Powder | | color | Light yellow | | Appearance | Solid Powder | | BRN | 6668526 | | InChI | InChI=1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24) | | InChIKey | HUXIAXQSTATULQ-UHFFFAOYSA-N | | SMILES | C1=C2C(C=CC(Br)=C2)=CC(OP(O)(O)=O)=C1C(NC1=CC=CC=C1OC)=O | | CAS DataBase Reference | 1919-91-1 | | EPA Substance Registry System | 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)- (1919-91-1) |
| | NAPHTHOL AS-BI PHOSPHATE Usage And Synthesis |
| Description | Naphthol AS-BI-phosphate is a fluorogenic substrate for acid and alkaline phosphatases. Naphthol AS-BI-phosphate is cleaved to release the fluorescent moiety naphthol AS-BI (N-ASBI), which can be used to quantify the activity of acid and alkaline phosphatases. Acid and alkaline phosphatases are commonly used for diagnosis of diseases. | | Chemical Properties | Light yellow crystalline powder | | Uses | Naphthol AS-BI phosphate is suitable to use:- in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
- as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
- to perform alkaline phosphatase reaction
', 'Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells. | | Biological Activity | Naphthol AS-BI can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells. |
| | NAPHTHOL AS-BI PHOSPHATE Preparation Products And Raw materials |
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