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| | Biotin-PEG4-Dde-TAMRA-PEG3-Azide Basic information |
| | Biotin-PEG4-Dde-TAMRA-PEG3-Azide Chemical Properties |
| solubility | DMSO, DMF | | pka | 3.504±0.36(predicted) | | Appearance | Red amorphous solid |
| | Biotin-PEG4-Dde-TAMRA-PEG3-Azide Usage And Synthesis |
| Description | Dde TAMRA Biotin Azide is a trifunctional, cleavable probe that allows for efficient recovery of avidin-bound protein complexes in affinity-based assays. This reagent contains a fluorescent dye (TAMRA) and biotin moiety linked to azide group through a spacer arm containing a hydrazine-cleavable Dde moiety. Under mild conditions (2% aqueous hydrazine), the Dde liner is cleaved, releasing the biotin tag and any avidin conjugate bound to it. | | Uses | Biotin-PEG4-Dde-TAMRA-PEG3-Azide is a dye derivative of TAMRA (HY-135640) modified with a cleavable biotin group. Biotin-PEG4-Dde-TAMRA-PEG3-Azide contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups. | | IC 50 | Cleavable Linker | | References | [1] Beck A, et al. Strategies and challenges for the next generation of antibody-drug conjugates. Nat Rev Drug Discov. 2017 May;16(5):315-337. DOI:10.1038/nrd.2016.268 |
| | Biotin-PEG4-Dde-TAMRA-PEG3-Azide Preparation Products And Raw materials |
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