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Boao Pike |
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| | NF-κB (p65) Monoclonal Antibody (Clone 112A1021) Basic information |
| | NF-κB (p65) Monoclonal Antibody (Clone 112A1021) Chemical Properties |
| | NF-κB (p65) Monoclonal Antibody (Clone 112A1021) Usage And Synthesis |
| Description | NF-κB p65 is a ubiquitously expressed transcription factor that is a subunit of the NF-κB complex and is encoded by the RELA gene in humans. It is composed of an N-terminal Rel homology domain, which contains the nuclear localization signal (NLS), and mediates dimerization, nuclear localization, and DNA and protein interactions, and two C-terminal transactivation domains that are subject to a variety of post-translational modifications and regulate the transcriptional activity of p65. NF-κB p65 regulates the expression of a large number of genes in response to inflammatory and environmental cues that play critical roles in innate and adaptive immunity and cellular differentiation. Silencing of Rela induces tumor cell apoptosis in a murine Lewis lung carcinoma model, and RELA silencing in THP-1 monocytes decreases secreted levels of IL-1β and TNF-α induced by LPS. Genome-wide deletion of Rela in mice is embryonic lethal. NF-κB p65 is overexpressed in the inflamed joints of patients with rheumatoid arthritis, and naïve CD4 T cells isolated from the whole blood of patients with multiple sclerosis have increased phosphorylation of NF-κB p65. Cayman’s NF-κB (p65) Monoclonal Antibody (Clone 112A1021) can be used for flow cytometry (FC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes NF-κB (p65) from human, mouse, and rat samples. | | References | [1] JENNIFER M PETERSON Denis C G Nadine Bakkar. NF-κB signaling in skeletal muscle health and disease.[J]. Current Topics in Developmental Biology, 2011, 96: 85-119. DOI: 10.1016/b978-0-12-385940-2.00004-8
[2] SIVAGAMI GIRIDHARAN M S. Mechanisms of NF-κB p65 and strategies for therapeutic manipulation.[J]. Journal of Inflammation Research, 2018, 11: 407-419. DOI: 10.2147/jir.s140188
[3] Y QU R W X Zhang. Knockdown of NF-κB p65 subunit expression suppresses growth of nude mouse lung tumor cell xenografts by activation of Bax apoptotic pathway.[J]. Neoplasma, 2015, 62 1: 34-40. DOI: 10.4149/neo_2015_005
[4] CHUNTING WU. SiRNA directed against NFκB inhibits mononuclear macrophage cells releasing proinflammatory cytokines in vitro.[J]. Molecular medicine reports, 2017: 9060-9066. DOI: 10.3892/mmr.2017.7715
[5] AMER A. BEG David B. An Essential Role for NF-κB in Preventing TNF-α-Induced Cell Death[J]. Science, 1996, 274 5288. DOI: 10.1126/science.274.5288.782
[6] MAKAROV S. NF-kappaB in rheumatoid arthritis: a pivotal regulator of inflammation, hyperplasia, and tissue destruction[J]. Arthritis Research, 2001, 40 1: 200-206. DOI: 10.1186/ar300
[7] WILLIAM J. HOUSLEY. Genetic variants associated with autoimmunity drive NFκB signaling and responses to inflammatory stimuli[J]. Science Translational Medicine, 2015, 7 291. DOI: 10.1126/scitranslmed.aaa9223 |
| | NF-κB (p65) Monoclonal Antibody (Clone 112A1021) Preparation Products And Raw materials |
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