5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮
| 中文名称 | 5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮 |
|---|---|
| 中文同义词 | 5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮;5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮|||SMI-4A;5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮,10 MM DMSO 溶液 |
| 英文名称 | SMI-4a |
| 英文同义词 | 5-[[3-(Trifluoromethyl)phenyl]methylene]-2,4-thiazolidinedione;SMI-4a ≥95%(NMR);CS-627;SMI-4q;SMI-4a5-[[3-(Trifluoromethyl)phenyl]methylene]-2,4-thiazolidinedione;2,4-Thiazolidinedione, 5-[[3-(trifluoromethyl)phenyl]methylene]-;2 Kinase Inhibitor V;5-(3-(Trifluoromethyl)benzylidene)thiazolidine-2,4-dione |
| CAS号 | 327033-36-3 |
| 分子式 | C11H6F3NO2S |
| 分子量 | 273.23 |
| EINECS号 | 200-258-5 |
| 相关类别 | 对照品 |
| Mol文件 | 327033-36-3.mol |
| 结构式 | ![5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮 结构式](CAS/20150408/GIF/327033-36-3.gif) |
5-[[3-(三氟甲基)苯基]亚甲基]-2,4-噻唑烷二酮 性质
| 熔点 | 181-183℃ (methanol ) |
|---|---|
| 密度 | 1.530±0.06 g/cm3(Predicted) |
| 储存条件 | 2-8°C |
| 溶解度 | ≥ 9.4mg/mL，溶于 DMSO |
| 酸度系数(pKa) | 7.26±0.20(Predicted) |
| 形态 | 白色至类白色固体。 |
| 颜色 | light beige |
| InChI | 1S/C11H6F3NO2S/c12-11(13,14)7-3-1-2-6(4-7)5-8-9(16)15-10(17)18-8/h1-5H,(H,15,16,17)/b8-5+ |
| InChIKey | NGJLOFCOEOHFKQ-VMPITWQZSA-N |
Pim
TCS-PIM-1-4a (10 μM; 24-48 hours; 6812/2 cells and Jurkat cells) treatment increases the population of cells in the G1 phase from 44.3% to 68.4% and from 56.2% to 67.1% in 6812/2 and Jurkat, respectively. S-phase cells are decreased in 6812/2, whereas only small changes are seen in Jurkat cells consistent with the lesser G1 block.
TCS-PIM-1-4a (5 μM; 6 hours; 6812/2 cells and Jurkat cells) induces cell death by the induction of apoptosis.
TCS-PIM-1-4a (5 μM; 4-8 hours; 6812/2 cells and Jurkat cells) prevents the increase in 4E-BP1 protein levels and inhibits mTOR-directed phosphorylation on Thr37/46.
Cell Cycle Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 10 μM |
| Incubation Time: | 24 hours, 48 hours |
| Result: | Induced cell-cycle arrest. |
Apoptosis Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 5 μM |
| Incubation Time: | 6 hours |
| Result: | Led to an increase in the number of the cells positive for annexin V and negative for PI from 8.25% in the control to 21.85%. |
Western Blot Analysis
| Cell Line: | 6812/2 cells and Jurkat cells |
| Concentration: | 10 μM |
| Incubation Time: | 4 hours, 8 hours |
| Result: | Prevented the increase in 4E-BP1 protein levels and inhibited mTOR-directed phosphorylation on Thr37/46. |
TCS-PIM-1-4a (SMI-4a; 60 mg/kg; oral gavage; twice daily; for 21 days; nu/nu nude mice) treatment causes a significant delay in the tumor growth without any change in the weight, blood counts, or chemistries.
| Animal Model: | 18 Nu/nu nude mice with 6812/2 murine pre–T-LBL cells |
| Dosage: | 60 mg/kg |
| Administration: | Oral gavage; twice daily; for 21 days |
| Result: | Caused a significant delay in the tumor growth. |