| Company Name: |
Sichuan Kulinan Technology Co., Ltd
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| Tel: |
400-1166-196 18981987031 |
| Email: |
cdhxsj@163.com |
| Products Intro: |
Product Name:SYBR GREEN II
CAS:172827-25-7
Purity:99% HPLC Package:10g;50g;100g;500g;1kg;5kg;25kg
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| Company Name: |
Nanjing Pars Biochem CO.,Ltd
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| Tel: |
18252001943 |
| Email: |
info@pariselements.com |
| Products Intro: |
Product Name:SUPER Green II RNA gel stain *10,000× concentrate in DMSO* (Electrophoresis Grade)
CAS:172827-25-7
Package:50UG/
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| Company Name: |
Guangzhou Younan Technology Co., Ltd
|
| Tel: |
020-82000279 18988968278 |
| Email: |
YN_research@163.com |
| Products Intro: |
Product Name:5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers*
CAS:172827-25-7
Purity:0.97 Package:5mg;10mg;25mg;50mg;100mg;250mg;500mg;1g
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| Company Name: |
Energy Chemical
|
| Tel: |
021-58432009 400-005-6266 |
| Email: |
marketing1@energy-chemical.com |
| Products Intro: |
Product Name:SYBR(R) Green II RNA gel stain 10,000 x in DMSO
CAS:172827-25-7
Purity:NULL Package:0.5ml Remarks:NULL
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| | SYBR GREEN II Basic information |
| Product Name: | SYBR GREEN II | | Synonyms: | SYBR GREEN II;SYBR?Green II RNA gel stain;SYBR(R) Green II RNA gel stain 10,000 x in DMSO;Green II RNA gel stain;5(6)-CR110 [5-(and 6)-Carboxyrhodamine 110] *Mixed isomers*;SYBR(R) GREEN II NUCLEIC ACID GEL STAIN;SYBR Green Ⅱ;SYBR? Green II RNA gel stain | | CAS: | 172827-25-7 | | MF: | | | MW: | 0 | | EINECS: | 200-664-3 | | Product Categories: | | | Mol File: | Mol File | ![SYBR GREEN II Structure]() |
| | SYBR GREEN II Chemical Properties |
| Fp | 87℃ | | storage temp. | −20°C | | Appearance | dark orange solution |
| RIDADR | NA 1993 / PGIII | | WGK Germany | 1 | | F | 3 |
| | SYBR GREEN II Usage And Synthesis |
| Description | ssGreen® is one of the most sensitive stains for post-electrophoresis staining of RNA and single-stranded DNA (ssDNA) in agarose or polyacrylamide gels. The fluorescence quantum yield of the ssGreen/RNA complex is more than 7× higher than that of the ethidium bromide/RNA complex. Although ssGreen is not selective for RNA staining, the dye exhibits about 1.5× greater quantum yield when bound to RNA than double-stranded DNA, which makes it unique among all nucleic acid dyes.
ssGreen staining is compatible with denaturing gels. On agarose/formaldehyde and polyacrylamide/urea gels, the sensitivity of ssGreen is slightly reduced but still superior to that of ethidium bromide. Staining agarose/formaldehyde gels with ssGreen does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.
Staining gels with ssGreen has fewer steps than those with ethidium bromide. Because the fluorescence of ssGreen/RNA complexes is not quenched by formaldehyde or urea, there is no need to wash these denaturants out of gels before staining. Also, ssGreen stain has a low intrinsic fluorescence, allowing gel viewing and photographing without preliminary removing unbound dye. | | Uses | SYBR(R) Green II RNA gel stain has been used:
- for the quantification of ribonucleic acid (RNA) in Escherichia coli
- for staining 5μ-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis
- to stain formaldehyde agarose gels to visualize RNA bands
| | Abs/Em Maxima | 483 (complex)/518 (complex) nm |
| | SYBR GREEN II Preparation Products And Raw materials |
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