AF488-dUTP

AF 488 dUTP is a nucleotide labeled with the fluorescent dye AF 488, used to synthesize labeled DNA probes. Nucleotides can be incorporated into nucleic acid using standard molecular biology techniques such as nick translation, random primer labeling, reverse transcription, PCR, and end-labeling with terminal deoxynucleotidyl transferase. Labeled DNA probes can be used for techniques such as FISH, microarrays, and blotting.

AF 488 is a fluorescent dye that is insensitive to pH in the range from 4 to 10. AF 488 has absorption maxima at 495 nm and emission maxima at 519 nm, which corresponds to the green region of the spectrum.