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| | MYOGLOBIN Basic information |
| | MYOGLOBIN Chemical Properties |
| storage temp. | −20°C | | form | buffered aqueous solution |
| | MYOGLOBIN Usage And Synthesis |
| Definition | A globular protein occurringwidely in muscle tissue as anoxygen carrier. It comprises a singlepolypeptide chain and a haem group, which reversibly binds a moleculeof oxygen. This is only relinquishedat relatively low externaloxygen concentrations, e.g. duringstrenuous exercise when muscle oxygendemand outpaces supply fromthe blood. Myoglobin thus acts as anemergency oxygen store. | | Purification Methods | Myoglobin is purified by CM-cellulose chromatography and Sephadex G-50 followed by chromatography on Amberlite IRC-50 Type III or BioRex 70 (<400mesh). The crystalline product as a paste in saturated (NH4)2SO4 at pH 6.5-7.0 may be stored at 4o for at least 4years unchanged, but must not be kept in a freezer. [Anres & Atassi Biochemistry 12 942 1980, Edmundson Biochemical Preparations 12 41 1968.] |
| | MYOGLOBIN Preparation Products And Raw materials |
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