|
|
| | N-(2-Acetamido)iminodiacetic acid Basic information |
| | N-(2-Acetamido)iminodiacetic acid Chemical Properties |
| Melting point | 219 °C (dec.)(lit.) | | Boiling point | 325.64°C (rough estimate) | | density | 1.4957 (rough estimate) | | vapor pressure | 0Pa at 25℃ | | refractive index | 1.5010 (estimate) | | storage temp. | room temp | | solubility | 1 M NaOH: 0.05 M at 20 °C, clear, colorless | | form | Powder | | color | White to almost white | | PH | 1.5-3.0 (25℃, 0.01M in H2O) | | PH Range | 6.0 - 7.2 | | pka | 6.6(at 25℃) | | Water Solubility | soluble | | λmax | λ: 260 nm Amax: 0.20
λ: 280 nm Amax: 0.05 | | Merck | 14,147 | | BRN | 1787181 | | Major Application | diagnostic assay manufacturing | | InChI | 1S/C6H10N2O5/c7-4(9)1-8(2-5(10)11)3-6(12)13/h1-3H2,(H2,7,9)(H,10,11)(H,12,13) | | InChIKey | QZTKDVCDBIDYMD-UHFFFAOYSA-N | | SMILES | NC(=O)CN(CC(O)=O)CC(O)=O | | LogP | -3.12 at 20℃ | | CAS DataBase Reference | 26239-55-4(CAS DataBase Reference) | | EPA Substance Registry System | Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)- (26239-55-4) |
| Safety Statements | 24/25 | | WGK Germany | 3 | | TSCA | TSCA listed | | HazardClass | IRRITANT | | HS Code | 29241900 | | Storage Class | 13 - Non Combustible Solids | | Hazard Classifications | Acute Tox. 4 Oral |
| | N-(2-Acetamido)iminodiacetic acid Usage And Synthesis |
| Description | N-(2-Acetamido)iminodiacetic acid is a zwitterionic buffer used in biochemistry and molecular biology. It is one of the Good buffers developed in the 1960′s to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of ADA and its physical properties. The useful range of ADA buffer in aqueous solution is 6.0 - 7.2. | | Chemical Properties | N-(2-Acetamido)iminodiacetic acid is white to almost white powder | | Uses | Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4).Used to prepare immobilized pH gradients. | | Uses | N-(2-Acetamido)iminodiacetic acid is buffer for biological systems and chelator for metals. | | Uses | N-(2-Acetamido)iminodiacetic acid (ADA) could prevents oxidation and irreversible denaturation of proteins while conducting gel electrophoresis. Furthermore studies suggest that ADA interferes with BCA and Lowry protein assays. Also ADA often chelates metal ions such as Mn(II), Cu(II), Ni(II), Zn(II), and Co(II) in a 2:1 at or below physiological pH values. Moreover, ADA is soluble in sodium hydroxide and absorbs UV radiation in the range below 260nm. | | Application | ADA, or N-(2-Acetamido)iminodiacetic acid, can be used to study biological buffers and zwitterionic compounds. ADA has been used in a study to describe the application of scanning capacitively coupled contactless conductivity detection (SC(4)D) for the determination of pH dependant behaviour of two aminopolycarboxylates immobilised onto the surface of a monolithic capillary column.
Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4). Used to prepare immobilized pH gradients. | | Definition | ChEBI: 2,2'-[(2-amino-2-oxoethyl)imino]diacetic acid is a tricarboxylic acid amide that is a Good's buffer substance, pKa = 6.6 at 20 ℃. It is a dicarboxylic acid, a tricarboxylic acid amide and an ADA. It is functionally related to a nitrilotriacetic acid. It is a conjugate acid of a 2,2'-[(2-amino-2-oxoethyl)imino]diacetate(1-). | | Flammability and Explosibility | Not classified | | Purification Methods | Dissolve ADA in water, add one equivalent of NaOH solution (to final pH of 8-9), then acidify with HCl to precipitate the free acid. This is filtered off, washed with water and dried in vacuo. [Beilstein 4 IV 2441.] |
| | N-(2-Acetamido)iminodiacetic acid Preparation Products And Raw materials |
|