| Company Name: |
Merck Millipore
|
| Tel: |
1-400-889-1988 400-889-1988 |
| Email: |
bioscience@merckgroup.com |
| Products Intro: |
Product Name:Human IgM, Plasma
|
| Company Name: |
Enzo Biochem Inc
|
| Tel: |
Enzo Biochem Inc. 13797054060 |
| Email: |
Enzoname@qq.com |
| Products Intro: |
Product Name:Human IgM
|
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| Product Name: | HUMAN IGM | | Synonyms: | HUMAN IGM, POLYCLONAL;HUMAN IGM, MYELOMA;HUMAN IGM (MYELOMA), WHOLE MOLECULE;HUMAN IGM;IGM, HUMAN;IGM HUMAN CONTROL;HUMAN IGM, WHOLE MOLECULE;HUMAN IGM, PLASMA | | CAS: | | | MF: | | | MW: | 0 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File | ![HUMAN IGM Structure]() |
| | HUMAN IGM Chemical Properties |
| storage temp. | 2-8°C | | form | buffered aqueous solution |
| Hazard Codes | B,Xn | | Risk Statements | 22 | | WGK Germany | - | | Storage Class | 12 - Non Combustible Liquids |
| | HUMAN IGM Usage And Synthesis |
| Uses | Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml) and dot blot assay. Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.
IgM from human serum has been used
- in ELISA inhibition assay
- in immunoblotting
- as MW (molecular weight) standards
- in IgM binding assay
- to confirm the depletion of immunoglobulins
| | General Description | There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures. | | Biochem/physiol Actions | Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. | | Description | Human Immunoglobulin-M produced in human plasma having a molecular mass of 950kDa. | | Source | Human plasma | | Background | Immunoglobulin M (IgM) is a basic antibody produced by B cells. IgM is the first antibody to emerge in response to initial exposure to an antigen. IgM antibodies are found in the blood and lymph fluid and are the third most widespread serum Ig. Immunoglobulin M (IgM), being the 3rd most widespread serum Ig and exists in two forms- mostly as a pentamer (970kDa) but also as a hexamer. The pentameric IgM has 10 binding sites since each monomer has two antigen binding sites. Due to distance constraints in the hexameric complex, the J chain is found in pentameric IgM but not in the hexameric form. IgM antibodies, which appear early in the course of an infection, typically reappear to a smaller extent after additional exposure. IgM, as opposed to IgG antibodies, do not pass across the human placenta. These properties of IgM make it suitable for the diagnosis of infectious diseases. |
| | HUMAN IGM Preparation Products And Raw materials |
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