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Sigma-Aldrich |
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021-61415566 800-8193336 |
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orderCN@merckgroup.com |
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Skygen, Inc. |
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19852949191 19852949191 |
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| | PBR322 PLASMID DNA Basic information |
| Product Name: | PBR322 PLASMID DNA | | Synonyms: | Deoxyribonucleic acid, Plasmid (pBR 322 Plasmid) from E. coli RRI;PBR322 VECTOR;PBR325 PLASMID DNA;PBR322 PLASMID DNA;PLASMID PBR322 DEOXYRIBONUCLEIC ACID;PLASMID PBR322 DNA;DNA, PBR322;DEOXYRIBONUCLEIC ACID, PLASMID (PBR 322 PLASMID) | | CAS: | 93384-17-9 | | MF: | | | MW: | 0 | | EINECS: | 297-227-2 | | Product Categories: | | | Mol File: | Mol File | ![PBR322 PLASMID DNA Structure]() |
| | PBR322 PLASMID DNA Chemical Properties |
| storage temp. | -20°C | | form | lyophilized powder | | biological source | Escherichia coli |
| WGK Germany | 3 | | F | 3-10-21 | | Storage Class | 11 - Combustible Solids |
| | PBR322 PLASMID DNA Usage And Synthesis |
| Uses | Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication. | | Uses | Select for recombinant DNA. The Pst I and Pvu I cleavage sites in the ampicillin gene, or the Cla I, Hind III, BamH I, and Sal I sites in the tetracycline gene allow the insertion of foreign DNA fragments, and inactivation of one of these genes. pBR322 DNA has been used in to study artificial metallonuclease activity. | | Uses | pBR322 Plasmid DNA fromE. coliRRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide. | | General Description | Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.
The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I). | | Biochem/physiol Actions | This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. |
| | PBR322 PLASMID DNA Preparation Products And Raw materials |
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