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| | Cyclic GMP ELISA Kit Basic information |
| | Cyclic GMP ELISA Kit Chemical Properties |
| | Cyclic GMP ELISA Kit Usage And Synthesis |
| Description | The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2'' hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions. Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106 cells). | | References | [1] L CHEN J L M M N Salafranca. Cyclooxygenase inhibition decreases nitric oxide synthase activity in human platelets.[J]. American Journal of Physiology, 1997, 273 4: H1854-9. DOI: 10.1152/ajpheart.1997.273.4.h1854
[2] O V MILLER R R G. Modulation of platelet cyclic nucleotide content by PGE1 and the prostaglandin endoperoxide PGG2.[J]. Journal of cyclic nucleotide research, 1976, 2 2: 79-87.
[3] CHIN-CHUNG WU. YC-1 inhibited human platelet aggregation through NO-independent activation of soluble guanylate cyclase[J]. British Journal of Pharmacology, 1995, 116 3: 1973-1978. DOI: 10.1111/j.1476-5381.1995.tb16400.x
[4] TAKASHI ARIMA . Cyclic GMP Elevation by 5-Hydroxytryptamine Is Due to Nitric Oxide Derived from Endogenous Nitrosothiol in NG108-15 Cells[J]. Biochemical and biophysical research communications, 1996, 227 2: Pages 473-478. DOI: 10.1006/bbrc.1996.1531
[5] J. KOLB. Interleukin-4 stimulates cGMP production by IFN-gamma-activated human monocytes. Involvement of the nitric oxide synthase pathway.[J]. The Journal of Biological Chemistry, 1994, 40 1: 9811-9816. DOI: 10.1016/s0021-9258(17)36955-7
[6] MAIA DATUNASHVILI. Modulation of Hyperpolarization-Activated Inward Current and Thalamic Activity Modes by Different Cyclic Nucleotides.[J]. Frontiers in Cellular Neuroscience, 2018: 369. DOI: 10.3389/fncel.2018.00369
[7] ROGER FLORES-COSTA. The soluble guanylate cyclase stimulator IW-1973 prevents inflammation and fibrosis in experimental non-alcoholic steatohepatitis[J]. British Journal of Pharmacology, 2017, 175 6: 953-967. DOI: 10.1111/bph.14137
[8] ASMAA I. MATOUK. The Effect of Chronic Activation of the Novel Endocannabinoid Receptor GPR18 on Myocardial Function and Blood Pressure in Conscious Rats[J]. Journal of Cardiovascular Pharmacology, 2017, 69 1: 23-33. DOI: 10.1097/fjc.0000000000000438
[9] LINEU BALDISSERA JR. The soluble guanylyl cyclase activator BAY 60-2770 inhibits murine allergic airways inflammation and human eosinophil chemotaxis[J]. Pulmonary pharmacology & therapeutics, 2016, 41: Pages 86-95. DOI: 10.1016/j.pupt.2016.11.001
[10] A. A. SYED. Cardioprotective Effect of Ulmus wallichiana Planchon in β-Adrenergic Agonist Induced Cardiac Hypertrophy[J]. Frontiers in Pharmacology, 2016, 7 1. DOI: 10.3389/fphar.2016.00510
[11] FRANCESCO IMPAGNATIELLO. Intraocular Pressure-Lowering Activity of NCX 470, a Novel Nitric Oxide-Donating Bimatoprost in Preclinical Models.[J]. Investigative ophthalmology & visual science, 2015, 56 11: 6558-6564. DOI: 10.1167/iovs.15-17190
[12] TAYLOR CLAYBAUGH. L-Arginine Supplementation in Type II Diabetic Rats Preserves Renal Function and Improves Insulin Sensitivity by Altering the Nitric Oxide Pathway.[J]. International Journal of Endocrinology, 2014, 2014: 171546. DOI: 10.1155/2014/171546
[13] LUIZ O LEIRIA. The soluble guanylyl cyclase activator BAY 60-2770 ameliorates overactive bladder in obese mice.[J]. Journal of Urology, 2014, 191 2: 539-547. DOI: 10.1016/j.juro.2013.09.020
[14] ACHIM H.P. KRAUSS . Ocular hypotensive activity of BOL-303259-X, a nitric oxide donating Prostaglandin F2α agonist, in preclinical models[J]. Experimental eye research, 2011, 93 3: Pages 250-255. DOI: 10.1016/j.exer.2011.03.001 |
| | Cyclic GMP ELISA Kit Preparation Products And Raw materials |
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