| Company Name: |
Shanghai Beiwanta Biotechnology Co., Ltd. Gold
|
| Tel: |
021-67187366 19901745723 |
| Email: |
info@bwtlab.com |
| Products Intro: |
Product Name:5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride)
CAS:90217-02-0
Purity:98% Package:100mg
|
|
| Product Name: | CTC | | Synonyms: | 5-Cyano-2,3-bis(4-methylphenyl)tetrazolium chloride;CTC;CYANODITOLYL TETRAZOLIUM CHLORIDE;CTC 5-Cyano-2,3-ditolyl tetrazolium chloride;5-CYANO-2,3-DI-(P-TOLYL)TETRAZOLIUM CHLORIDE;5-CYANO-2,3-DITOLYL TETRAZOLIUM CHLORIDE;5-CYANO-2,3-BIS(4-METHYLPHENYL)-2H-TETRAZOLIUM CHLORIDE;2h-tetrazolium, 5-cyano-2,3-bis(4methylphenyl)-, Chloride | | CAS: | 90217-02-0 | | MF: | C16H14N5.Cl | | MW: | 311.77 | | EINECS: | | | Product Categories: | marker;Tetrazolium salt | | Mol File: | 90217-02-0.mol |  |
| Melting point | 225 ℃ | | storage temp. | 2-8°C | | solubility | Soluble in water | | form | powder | | color | Off-white | | λmax | 450nm (of formazan) | | BRN | 8954451 | | Biological Applications | NADPH-cytochrome P 450 reductase activity assays; detecting gamma-hydroxybutyric acid (GHB),metabolic activity,microorganisms; localizing glucose-6-phosphate dehydrogenase activity; monitoring respiratory activity; treating cancer | | InChI | InChI=1S/C16H15N5.ClH/c1-13-3-7-15(8-4-13)19-12-18-20(11-17)21(19)16-9-5-14(2)6-10-16;/h3-10,12H,1-2H3;1H | | InChIKey | BLCCXPMHHCWJHC-UHFFFAOYSA-N | | SMILES | N1(C2=CC=C(C)C=C2)C=NN(C#N)[NH+]1C1=CC=C(C)C=C1.[Cl-] |
| Hazard Codes | Xi | | Risk Statements | 36/37/38 | | Safety Statements | 26 | | WGK Germany | 3 | | F | 8 | | HS Code | 2933998090 | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Eye Irrit. 2
Skin Irrit. 2
STOT SE 3 |
| Description | 5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable. | | Uses | Redox dye producing a fluorescent formazan (only in solid state) upon reduction. It is used as cellular indicator of respiratory activity in cells, especiall for tracking P450 activity. | | Uses | 5-Cyano-2,3-di-p-tolyltetrazolium Chloride is a monotetrazolium redox dye that is used as a vital stain for actively respiring bacteria. Redox dye producing a fluorescent formazan (only in solid state) upon reduction; used as cellular indicator of respiratory activity in cells. | | References | [1] G G RODRIGUEZ. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria.[J]. Applied and Environmental Microbiology, 1992, 58 6: 1801-1808. DOI: 10.1128/aem.58.6.1801-1808.1992
[2] G. CARUSO E. C M Mancuso. Combined fluorescent antibody assay and viability staining for the assessment of the physiological states of Escherichia coli in seawaters[J]. Journal of Applied Microbiology, 2003, 95 2: 225-233. DOI: 10.1046/j.1365-2672.2003.01967.x
[3] M E SIERACKI J N T L Cucci. Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.[J]. Applied and Environmental Microbiology, 1999, 65 6: 2409-2417. DOI: 10.1128/aem.65.6.2409-2417.1999
[4] VISHVESH K BHUPATHIRAJU . Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria[J]. Journal of microbiological methods, 1999, 37 3: Pages 231-243. DOI: 10.1016/s0167-7012(99)00069-x |
| | CTC Preparation Products And Raw materials |
|