| Company Name: |
Sigma-Aldrich |
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
|
| | Acetyl-CoA synthetase Basic information |
| Product Name: | Acetyl-CoA synthetase | | Synonyms: | S-acetyl coenzyme A synthetase*from bakers yeast;Synthetase, acetyl coenzyme A;acetate coa ligase (amp forming);s-acetyl-coenzyme a synthetase from baker's yeast (s. cerevisiae);Acetate CoA ligase (AMP forming), Acetate thiokinase;ACETYL-COA SYNTHETASE;ACETATE: COA LIGASE;ACETATE THIOKINASE | | CAS: | 9012-31-1 | | MF: | | | MW: | 0 | | EINECS: | 232-729-7 | | Product Categories: | | | Mol File: | Mol File | ![Acetyl-CoA synthetase Structure]() |
| | Acetyl-CoA synthetase Chemical Properties |
| storage temp. | -20°C | | form | lyophilized powder | | Specific Activity | ≥3units/mg protein |
| WGK Germany | 3 | | Storage Class | 11 - Combustible Solids | | Hazard Classifications | Resp. Sens. 1 |
| | Acetyl-CoA synthetase Usage And Synthesis |
| Uses | S-Acetyl-coenzyme A synthetase may be used to study various metabolic pathways, such as glycolysis, gluconeogenesis, pyruvate metabolism and CO2 fixation. It may also be used in gene expression studies. | | Biochem/physiol Actions | Acetyl-coenzyme A synthetase catalyzes the production of acetyl-CoA. It is involved in histone acetylation in the nucleus. It may be involved in the growth of nonfermentable carbon sources such as glycerol. Acetyl-coenzyme A synthetase is induced by acetate, acetaldehyde and ethanol . | | Purification Methods | This enzyme has been purified by extraction into phosphate buffer pH 6.8-7.0 containing 2-mercaptoethanol and EDTA, protamine sulfate precipitation, polyethylene glycol fractionation, Alumina gel filtration, concentration by (NH4)2SO4 precipitation, Bio-Gel A-0.5m chromatography and DEAE-cellulose gradient chromatography. It has Km (apparent) 0.24mM (for acetate) and 0.035mM (for CoA); 1.2 mM for ATP and Mg2+ 4.0mM. [Frenkel & Kitchens Methods Enzymol 71 317 1981.] |
| | Acetyl-CoA synthetase Preparation Products And Raw materials |
|