Cyanine3.5 is a bright and photostable fluorophore, which is similar in its spectral characteristics to Cy3.5™ dye. Cyanine3.5 DBCO fluorescence maximum is at 604 nm and in the orange-red spectrum range.
Cyanine3.5 fluorescence is pH insensitive in the range from 4 to 10 and can be detected in the spectrum range with a low level of autofluorescence of biological samples.
Cyanine3.5 DBCO (dibenzocyclooctyne) easily forms conjugates with azide derivatives of biomolecules in copper-free Click Chemistry. The process runs without catalysts or increased temperature, resulting in stable triazoles.
Conjugates of biomolecules with Cyanine3.5 can be used for various microscopy assays, including FRET-microscopy, and in proteomics.