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ARRAKIS INDUSTRIES LLP
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Product Name:PHENOL:CHLOROFORM:ISO-AMYL ALCOHOL
CAS:136112-00-0
Purity:99% Package:1 kg,5 kg, 10 kg,25kg and 1 MT
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| Company Name: |
chemmole Gold
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| Products Intro: |
Product Name: Phenol -chloroform - isoamyl alcohol mixture
CAS:136112-00-0
Purity:25:24:1 Package:250ml/RMB 850
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PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 manufacturers
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| | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Basic information |
| Product Name: | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 | | Synonyms: | Phenol/Chloroform/Isoamyl alcohol (25:24:1),stabilized,for molecular biology,DNAse, RNAse;Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized;Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, DNAse, RNAse and Protease free, pH 7.8-8.2, for molecular biology;Phenol/ChloroforM/IsoaMyl alcohol (25:24:1), stabilized, for Molecular biology, DNAse, RNAse and Protease free, pH 7.8-8.2;Phenol mixt. with 3-methyl-1-butanol and trichloromethane;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1;PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1, FOR MOL. BIOLOGY;PHENOL:CHLOROFORM:ISOAMYL ALC. 125:24:1, FOR MOL. BIOLOGY | | CAS: | 136112-00-0 | | MF: | C12H19Cl3O2 | | MW: | 301.63706 | | EINECS: | | | Product Categories: | Acids and BasesBiochemicals and Reagents;AlphabeticalMolecular Biology;BioUltraMolecular Biology;Biochemicals and Reagents;Denaturation;DNA&RNA Purification;Molecular Biology Reagents;Reagents | | Mol File: | 136112-00-0.mol |  |
| | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Chemical Properties |
| density | 1.29 g/mL at 20 °C | | Fp | 79 °C | | storage temp. | 2-8°C | | form | Liquid | | PH | 7.7-8.3(H2O-phase, after extraction with H2O, 1:1) | | Water Solubility | Soluble in water. | | λmax | λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1 | | InChI | InChI=1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H | | InChIKey | ZYWFEOZQIUMEGL-UHFFFAOYSA-N | | SMILES | ClC([H])(Cl)Cl.O([H])C([H])([H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H].O([H])C1C([H])=C([H])C([H])=C([H])C=1[H] | | Absorption | cut-off at 360nm |
| Hazard Codes | T | | Risk Statements | 23/24/25-34-40-48/20/21/22-68-63 | | Safety Statements | 26-36/37/39-45 | | RIDADR | UN 2810 6.1/PG 2 | | WGK Germany | 3 | | F | 8-10-23 | | HazardClass | 8 | | HS Code | 29420000 | | Storage Class | 6.1A - Combustible acute toxic Cat. 1 and 2
very toxic hazardous materials | | Hazard Classifications | Acute Tox. 3 Oral
Acute Tox. 4 Dermal
Acute Tox. 4 Inhalation
Aquatic Chronic 2
Carc. 2
Eye Dam. 1
Muta. 2
Repr. 2
Skin Corr. 1B
STOT RE 1 Oral
STOT RE 2
STOT SE 3 |
| | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Usage And Synthesis |
| Chemical Properties | clear yellow solution | | Uses | Phenol:Chloroform:Isoamyl alcohol 25:24:1 is used in the extraction of DNA for the generation of DNA minisatellite fingerprints. It is also used for purifying RNA. | | Biological Activity | When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction. | | Synthesis | Examples of the application of phenol-chloroform-isoamyl alcohol mixture are as follows:
(1) For the extraction of genomic DNA of a glycoconjugate-producing bacterium suitable for whole genome sequencing, extraction buffer and lysozyme were added to a culture of glycoconjugate-producing bacterium, which was left to stand for 10 min at 37C, then proteinase K was added, and left to stand for 1 h at 65C; a mixture of phenol-chloroform-isoamyl alcohol was added, and extracted; a mixture of chloroform-isoamyl alcohol was added, and extracted; cold ethanol and sodium acetate were added, and the precipitate was washed by ethanol and dried at room temperature, and RNA was added to dissolve it. centrifugation, the precipitate was washed with ethanol and dried at room temperature, and solubilized by adding DNA extraction buffer; add RNAase, and let it stand for 10 min at 37; add PEG6000, mix well, and let it stand for 10 min at 50; add a mixed solution of phenol-chloroform-isoamyl alcohol, and extract; add a mixed solution of chloroform-isoamyl alcohol, and extract; add a solution of cold ethanol and sodium acetate, and centrifugation, the precipitate was washed with ethanol and dried at room temperature, and the precipitate was washed with ethanol. After drying at room temperature, add ddH2O or TE buffer to dissolve, which is the extracted DNA solution.
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| | PHENOL:CHLOROFORM:ISOAMYL ALC. 25:24:1 Preparation Products And Raw materials |
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