Anti-IL6R抗体

兔多克隆抗体to IL-6RRabbit与反应: HumanUnconjugatedThis antibody was obtained from a rabbit immunized with purified, recombinant Mouse IL-6R / CD126 (rM IL-6R / CD126; Catalog#50280-M08H; NP_034689.2; Ile44-Glu357).适用于: WB, IHC-P, ELISA

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody (ab128008)

Paraffin-embedded human spleen tissue stained for IL6R with ab128008 at 2.5 µg/ml in immunohistochemical analysis.

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.双抗体夹心法测定标本中IL6R水平。用纯化的IL6R抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入IL6R，再与HRP标记的IL6R抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的IL6R呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中IL6R浓度。
Anti-IL6R抗体是一类可以特异性结合IL6R的单克隆抗体，主要用于检测IL6R的Western Blot、IHC-P、IF、ELISA、Co-IP等多种免疫学实验。托珠单抗是人源化IL-6受体（IL-6R）的单克隆抗体，可抑制促IL-6信号通路，阻止外周B细胞异常活化，在NMOSD患者中的疗效亟待证实。硫唑嘌呤是一种抑制嘌呤核苷酸生物合成的免疫抑制药，是目前作为治疗NMOSD的一线药物之一，但具有致白细胞降低和肝功能异常等副作用。
NMOSD是一种高复发、高致残性的中枢神经系统自身免疫性疾病，以反复发生的视神经炎和脊髓炎为主要特征。
目前对NMOSD的治疗和世界上其他国家相似，主要以经典的免疫抑制剂如硫唑嘌呤、吗替麦考酚酯等和CD20单抗利妥昔单抗治疗为主。即使应用上述药物，但仍有25-50%的患者在随访期间出现疾病的复发。每次复发会导致疾病残疾的累积，患者的神经系统功能无法恢复到复发前的水平。