Protease/Phosphatase Inhibitor Cocktail (100X)

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Protease/Phosphatase Inhibitor Cocktail (100X) Suppliers list
Company Name: Shanghai Universal Biotech Co.,Ltd  
Tel: 15921930842 15921930842
Email: yh-wang@univ-bio.com
Protease/Phosphatase Inhibitor Cocktail (100X) Basic information
Description Background
Product Name:Protease/Phosphatase Inhibitor Cocktail (100X)
Synonyms:Protease/Phosphatase Inhibitor Cocktail (100X)
CAS:
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MW:0
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Protease/Phosphatase Inhibitor Cocktail (100X) Structure
Protease/Phosphatase Inhibitor Cocktail (100X) Chemical Properties
Safety Information
MSDS Information
Protease/Phosphatase Inhibitor Cocktail (100X) Usage And Synthesis
DescriptionWhen diluted in lysis buffer to a final concentration of 1X the Protease/Phosphatase Inhibitor Cocktail prevents protein degradation and dephosphorylation by endogenous proteases and phosphatases present in the whole cell extract. The 100X cocktail is a clear light yellow to light green liquid.
BackgroundDynamic protein phosphorylation is a key cellular signaling mechanism by which a broad spectrum of cellular processes is regulated. In order to study the phosphorylation status of specific target proteins the phosphorylated residue of interest must remain intact. When cells are lysed to make whole cell extracts, a loss of normal cellular signaling regulation occurs, and phosphatases within the cell extract are free to dephosphorylate proteins in an uncontrolled manner. The addition of phosphatase inhibitors to the cell lysis buffer aids in the preservation of phosphorylated residues at the time of cell disruption.This same loss of normal cellular control when generating whole cell extracts also leads to uncontrolled degradation of proteins by endogenous proteases. The addition of protease inhibitors to the cell lysis buffer aids in the preservation of target proteins in the cell extract.
Protease/Phosphatase Inhibitor Cocktail (100X) Preparation Products And Raw materials
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